pLenti-EF1a-GFP-2A-Puro

                            LOCUS       dna                      9078 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     ORF             1775..2755
                     /sequence="ORF_4 rf(6)"
     Promoter        1838..3015
                     /gene="EF1a prom"
     misc_binding    2802..2807
                     /dbxref="REBASE:XhoI"
     misc_binding    3087..3092
                     /dbxref="REBASE:EcoRI"
     misc_binding    3111..3116
                     /dbxref="REBASE:HincII"
     misc_binding    3117..3122
                     /dbxref="REBASE:EcoRV"
     misc_binding    3129..3136
                     /dbxref="REBASE:NotI"
     misc_binding    3138..3143
                     /dbxref="REBASE:BamHI"
     misc_binding    3144..3149
                     /dbxref="REBASE:XbaI"
     Promoter        3150..3418
                     /gene="SV40 prom"
     Rep_Origin      3317..3394
                     /gene="SV40 origin"
     misc_binding    3397..3409
                     /dbxref="REBASE:SfiI"
     ORF             3466..4875
                     /sequence="ORF_2 rf(1)"
     Marker          4276..4875
                     /gene="puro marker"
     Other Gene      5485..5537
                     /gene="delta_U3 other"
     Other Gene      5538..5718
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      6225..6844
                     /gene="pBR322 origin"
     Promoter        7102..7151
                     /gene="NEOKAN prom"
     misc_binding    7209..7214
                     /dbxref="REBASE:BclI"
     ORF             7240..8034
                     /sequence="ORF_3 rf(1)"
     Marker          7243..8031
                     /gene="NTP_II marker"
     Rep_Origin      8139..8445
                     /gene="f1 origin"
     Other Gene      8500..8601
                     /gene="RITR other"
     Other Gene      8500..8601
                     /gene="LITR other"
     Other Gene      8681..8829
                     /gene="Encap other"
BASE COUNT    2129 a   2324 c   2603 g   2022 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagggagt gggaattggc tccggtgccc gtcagtgggc     
     1861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg     
     1921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     
     1981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     
     2041 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg     
     2101 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct gcagtacgtg     
     2161 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa     
     2221 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg     
     2281 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa     
     2341 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc     
     2401 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg     
     2461 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg     
     2521 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc     
     2581 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg     
     2641 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg     
     2701 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac     
     2761 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg     
     2821 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg     
     2881 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt     
     2941 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca     
     3001 tttcaggtgt cgtgaggaat ttcgactgct agctggtacc tgaattgagt acttacgtag     
     3061 gtaccccagt gtggtggcct gcaggtgaat tcactagtac cggtaggcct gtcgacgata     
     3121 tcgggcccgc ggccgctgga tcctctagag gtgtggaaag tccccaggct ccccagcagg     
     3181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg     
     3241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc     
     3301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca     
     3361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt     
     3421 ccagaagtag tgaggaggct tttttggagg cctgtcgagg ccaccatgga gagcgacgag     
     3481 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag     
     3541 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg     
     3601 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac     
     3661 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc     
     3721 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac     
     3781 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc     
     3841 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc     
     3901 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc     
     3961 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc     
     4021 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag     
     4081 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc     
     4141 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga     
     4201 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag     
     4261 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac     
     4321 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac     
     4381 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg     
     4441 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc     
     4501 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg     
     4561 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg     
     4621 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag     
     4681 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg     
     4741 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc     
     4801 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc     
     4861 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga     
     4921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg     
     4981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc     
     5041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc     
     5101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt     
     5161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt     
     5221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg     
     5281 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg     
     5341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg     
     5401 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt     
     5461 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc     
     5521 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg     
     5581 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag     
     5641 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag     
     5701 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg     
     5761 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca     
     5821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt     
     5881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga     
     5941 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac     
     6001 ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct     
     6061 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     
     6121 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     
     6181 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     
     6241 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     
     6301 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     
     6361 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     
     6421 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     
     6481 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     
     6541 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     
     6601 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     
     6661 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     
     6721 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     
     6781 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     
     6841 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggcc     
     6901 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag     
     6961 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca     
     7021 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg     
     7081 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg     
     7141 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga     
     7201 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg     
     7261 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag     
     7321 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt     
     7381 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta     
     7441 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg     
     7501 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt     
     7561 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat     
     7621 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg     
     7681 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca     
     7741 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc     
     7801 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc     
     7861 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat     
     7921 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     
     7981 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg     
     8041 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc     
     8101 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag     
     8161 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga     
     8221 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc     
     8281 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa     
     8341 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt     
     8401 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc     
     8461 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atataccttt     
     8521 tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg     
     8581 ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac     
     8641 acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt gtacacagga     
     8701 agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg taaccgagta     
     8761 agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga ataattttgt     
     8821 gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta tttagaaaaa     
     8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg taactataac     
     8941 ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg caacatggta     
     9001 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg     
     9061 gaagtaaggt ggtacgat