piLenti-siRNA
Vector Name | piLenti-siRNA |
VectorType | Lentiviral siRNA Vector |
Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
Additional Information | No Additional Information |
Double-click on the features for more information
LOCUS dna 8221 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
Promoter 2440..2695
/gene="hU6 prom"
misc_binding 2709..2714
/dbxref="REBASE:XbaI"
misc_binding 2715..2720
/dbxref="REBASE:EcoRV"
misc_binding 2735..2740
/dbxref="REBASE:EcoRI"
misc_binding 2741..2746
/dbxref="REBASE:XhoI"
misc_binding 2945..2950
/dbxref="REBASE:ApaI"
ORF 3507..4184
/sequence="ORF_3 rf(3)"
misc_binding 3570..3575
/dbxref="REBASE:KpnI"
misc_binding 3576..3581
/dbxref="REBASE:BamHI"
Marker 3588..4184
/gene="puro marker"
misc_binding 3634..3639
/dbxref="REBASE:XmaI"
misc_binding 3634..3639
/dbxref="REBASE:SmaI"
misc_binding 3914..3919
/dbxref="REBASE:StuI"
Other Gene 4794..4846
/gene="delta_U3 other"
Other Gene 4847..5027
/gene="HIV-1_5_LTR other"
Rep_Origin 5533..6152
/gene="pBR322 origin"
Promoter 6805..6854
/gene="NEOKAN prom"
misc_binding 6912..6917
/dbxref="REBASE:BclI"
ORF 6943..7737
/sequence="ORF_2 rf(1)"
Marker 6946..7734
/gene="NTP_II marker"
Rep_Origin 7842..8147
/gene="f1 origin"