2nd Generation Packaging System Mix
| Cat. No. | LV003 | ||
| Name | 2nd Generation Packaging System Mix | ||
| Unit | 200µl | ||
| Description |
For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest, 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a wildtype 5' LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5' LTR. All the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5'-LTR.
Packaging Mix Components (200ul mix of 2 plasmids) : pLenti-P2A, pLenti-P2B
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| Note |
NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only. |
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| Promotions |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV003 |
Datasheet
| How do I convert a generation 3 lentiviral vector into a vector that can be used by generation 2 packaging system? | |
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All second generation packaging systems can package both second and third generation lentiviral expression vectors.
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| Is it possible to make our own plasmid preparations from the packaging mix and then use them in transfections? | |
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Unfortunately it is not possible to amplify these plasmids as they are provided in a pre-made mixture.
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| Can I use 2nd Generation Packaging System for generation of lentivirus for pLKO.1 ShRNA knockdown vectors? | |
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If you are using lentiviral vectors that are compatible with a second generation packaging system then it should be fine, however, we have not tested this in house.
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| Which system should I use to package my TAT dependent vector? | |
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TAT dependent vectors will need to be packaged using a 2nd generation system (abm Cat LV003). 3rd generation packaging systems (abm Cat. LV053) will not be compatible with this type of vector.
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| What is the titer for viruses made by Cat# LV003 with 293T cells? | |
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For both our 2nd or 3rd generation packaging mixes, the crude viral supernatant (i.e. not concentrated nor purified) titer will be around 10^6IU/ml.
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10.1038\/s41565-020-0636-2\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1158\/1940-6207.CAPR-15-0338\u0022 rel=\u0022noopener\u0022\u003E02\u003C\/a\u003E\u003C\/span\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003EAXL-initiated paracrine activation of pSTAT3 enhances mesenchymal and vasculogenic supportive features of tumor-associated macrophages.\u003Cbr \/\u003E\u003Cem\u003E\u003Cbr \/\u003EHung C. et al. \u003Cbr \/\u003ECell Reports (2022)\u003C\/em\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/doi.org\/10.1016\/j.celrep.2023.113067\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1016\/j.celrep.2023.113067\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1136\/gutjnl-2015-309359\u0022 rel=\u0022noopener\u0022\u003E03\u003C\/a\u003E\u003C\/span\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003ERBFOX2 deregulation promotes pancreatic cancer progression and metastasis through alternative splicing.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cem\u003EMaurin M. et al.\u003Cbr \/\u003ENature Communications (2023) \u003C\/em\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/doi.org\/10.1038\/s41467-023-44126-w\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1038\/s41467-023-44126-w\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\u003C\/p\u003E","meta_title":"Virus Packaging Mixes","meta_keywords":"Virus Packaging Mixes, qPCR Virus Titer Kits, Virus Transduction Enhancer, Virus Purification Kits","meta_description":"abm\u2019s proprietary packaging mixes and highly efficient protocols based on years of experience permit rapid production of recombinant viral particles with high titer and purity.","deleted_at":null,"enable":"Y","parent_list":"8","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":16618,"cat_no_base":"LV003","parent_id":595,"description":"\u003Cp\u003EFor the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest, 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G).\u003C\/p\u003E\n\u003Cp\u003EIn general, lentiviral vectors with a wildtype 5\u0027 LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5\u0027 LTR.\u003C\/p\u003E\n\u003Cp\u003EAll the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5\u0027-LTR.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EPackaging Mix Components (200ul mix of 2 plasmids) :\u0026nbsp;\u003C\/strong\u003E \u0026nbsp;\u003Ca href=\u0022\/vector\/pLenti-P2A\u0022\u003EpLenti-P2A\u003C\/a\u003E, \u003Ca href=\u0022\/vector\/pLenti-P2B\u0022\u003EpLenti-P2B\u003C\/a\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003ESelection: \u003C\/strong\u003EAmpicillin (Bacterial Selection)\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EAmount:\u003C\/strong\u003E 200 \u0026mu;l (100 \u0026micro;g mixture of pLenti-P2A and pLenti-P2B)\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EAppearance:\u003C\/strong\u003E Liquid\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EStorage Temperature:\u003C\/strong\u003E -20\u0026deg;C or below (shipped at ambient temperature)\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EShelf Life:\u003C\/strong\u003E Up to 1 year (when at -20\u0026deg;C or below in a frost-free freezer)\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EUser Manual:\u003C\/strong\u003E Refer to website for downloadable \u003Ca href=\u0022\/uploads\/document\/A4_lenti_packaging_2023_V2.pdf\u0022\u003ELentivirus Packaging Protocol\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":"Not for diagnostic use.","storage_condition":null,"product_volume":null,"reporter":null,"safeview_series":null,"source_catno":"DNA Jennie - Packaged by Michael, Michael may have stock so check with him first.","stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"Chemical\/Vector Freezer- RACK-3 extra stock in shelf level 1","note":"\u003Cp\u003ENOT FOR RESALE without prior written consent of ABM. 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#ffffff;\u0022\u003E.\u003C\/span\u003E\u003C\/div\u003E"},"maps":[{"id":127,"name":"pLenti-P2A","sequence":"GAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGTCGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTATTTATTTATTTTTTAATTATTTTGTGCAGCGATGGGGGGGGGGGGGGGGGGGGCGCGCGCCGGGGGGGGCGGGGGGGGGCGGGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTTTATGGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCGCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTCCCACAGGTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGCTCCGGGAGGGCCCTTTGTGCGGGGGGAGCGGCTCGGGGGGTGCGTGCGTGTGTGTGTGCGTGGGGAGCGCCGCGTGCGGCTCCGCGCTGCCCGGCGGCTGTGAGCGCTGCGGGCGCGGCGCGGGGCTTTGTGCGCTCCGCAGTGTGCGCGAGGGGAGCGCGGCCGGGGGCGGTGCCCCGCGGTGCGGGGGGGGCTGCGAGGGGAACAAAGGCTGCGTGCGGGGTGTGTGCGTGGGGGGGTGAGCAGGGGGTGTGGGCGCGTCGGTCGGGCTGCAACCCCCCCTGCACCCCCCTCCCCGAGTTGCTGAGCACGGCCCGGCTTCGGGTGCGGGGCTCCGTACGGGGCGTGGCGCGGGGCTCGCCGTGCCGGGCGGGGGGTGGCGGCAGGTGGGGGTGCCGGGCGGGGCGGGGCCGCCTCGGGCCGGGGAGGGCTCGGGGGAGGGGCGCGGCGGCCCCCGGAGCGCCGGCGGCTGTCGAGGCGCGGCGAGCCGCAGCCATTGCCTTTTATGGTAATCGTGCGAGAGGGCGCAGGGACTTCCTTTGTCCCAAATCTGTGCGGAGCCGAAATCTGGGAGGCGCCGCCGCACCCCCTCTAGCGGGCGCGGGGCGAAGCGGTGCGGCGCCGGCAGGAAGGAAATGGGCGGGGAGGGCCTTCGTGCGTCGCCGCGCCGCCGTCCCCTTCTCCCTCTCCAGCCTCGGGGCTGTCCGCGGGGGGACGGCTGCCTTCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAGCTCCTGGGCAACGTGCTGGTTATTGTGCTGTCTCATCATTTTGGCAAAGAATTCGGGCCGCGTTGACGCGCACGGCAAGAGGCGAGGGGCGGCGACTGGTGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGATGGGAAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGGCAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCTGTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGATCATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACACCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGAAAAAAGCACAGCAAGCAGCAGCTGACACAGGACACAGCAATCAGGTCAGCCAAAATTACCCTATAGTGCAGAACATCCAGGGGCAAATGGTACATCAGGCCATATCACCTAGAACTTTAAATGCATGGGTAAAAGTAGTAGAAGAGAAGGCTTTCAGCCCAGAAGTGATACCCATGTTTTCAGCATTATCAGAAGGAGCCACCCCACAAGATTTAAACACCATGCTAAACACAGTGGGGGGACATCAAGCAGCCATGCAAATGTTAAAAGAGACCATCAATGAGGAAGCTGCAGAATGGGATAGAGTGCATCCAGTGCATGCAGGGCCTATTGCACCAGGCCAGATGAGAGAACCAAGGGGAAGTGACATAGCAGGAACTACTAGTACCCTTCAGGAACAAATAGGATGGATGACACATAATCCACCTATCCCAGTAGGAGAAATCTATAAAAGATGGATAATCCTGGGATTAAATAAAATAGTAAGAATGTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACCAAAGGAACCCTTTAGAGACTATGTAGACCGATTCTATAAAACTCTAAGAGCCGAGCAAGCTTCACAAGAGGTAAAAAATTGGATGACAGAAACCTTGTTGGTCCAAAATGCGAACCCAGATTGTAAGACTATTTTAAAAGCATTGGGACCAGGAGCGACACTAGAAGAAATGATGACAGCATGTCAGGGAGTGGGGGGACCCGGCCATAAAGCAAGAGTTTTGGCTGAAGCAATGAGCCAAGTAACAAATCCAGCTACCATAATGATACAGAAAGGCAATTTTAGGAACCAAAGAAAGACTGTTAAGTGTTTCAATTGTGGCAAAGAAGGGCACATAGCCAAAAATTGCAGGGCCCCTAGGAAAAAGGGCTGTTGGAAATGTGGAAAGGAAGGACACCAAATGAAAGATTGTACTGAGAGACAGGCTAATTTTTTAGGGAAGATCTGGCCTTCCCACAAGGGAAGGCCAGGGAATTTTCTTCAGAGCAGACCAGAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTTTGGGGAAGAGACAACAACTCCCTCTCAGAAGCAGGAGCCGATAGACAAGGAACTGTATCCTTTAGCTTCCCTCAGATCACTCTTTGGCAGCGACCCCTCGTCACAATAAAGATAGGGGGGCAATTAAAGGAAGCTCTATTAGATACAGGAGCAGATGATACAGTATTAGAAGAAATGAATTTGCCAGGAAGATGGAAACCAAAAATGATAGGGGGAATTGGAGGTTTTATCAAAGTAAGACAGTATGATCAGATACTCATAGAAATCTGCGGACATAAAGCTATAGGTACAGTATTAGTAGGACCTACACCTGTCAACATAATTGGAAGAAATCTGTTGACTCAGATTGGCTGCACTTTAAATTTTCCCATTAGTCCTATTGAGACTGTACCAGTAAAATTAAAGCCAGGAATGGATGGCCCAAAAGTTAAACAATGGCCATTGACAGAAGAAAAAATAAAAGCATTAGTAGAAATTTGTACAGAAATGGAAAAGGAAGGAAAAATTTCAAAAATTGGGCCTGAAAATCCATACAATACTCCAGTATTTGCC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