Cas9 Nickase D10A Protein
Cat. No.
K132
Unit
40µg (250pmol/ 25µL)
Price
$142.00
| Cat. No. | K132 | |
| Name | Cas9 Nickase D10A Protein | |
| Unit | 40µg (250pmol/ 25µL) | |
| Category | Cas Proteins & CRISPR Screening | |
| Description |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease.
|
|
| Cas Type | Nickase | |
| Cas Origin | spCas9 | |
| Cas Protein Marker | No GFP | |
| Concentration | 10 µM, 1.60 mg/ml | |
| Format General | Enzyme supplied with 10X Reaction Buffer | |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. | |
| Storage Condition | Store all components at -20°C. | |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K132 |
Datasheet
| Is the protein preparation free of other nucleases? | |
|
We do not observe any nucleic acid degradation in the presence of Cas9 D10A Nickase.
|
| Does this cas9 nickase protein contain a HIS-tag? | |
|
Yes, it does contain a His-tag.
|
| Does this cas9 nickase protein contain a Nuclear Localization Signal? | |
|
No, this Cas9 nickase protein does not have a Nuclear Localization Signal. If NLS is desired, we would recommend our Cas9 Nickase D10A NLS Protein (Cat#K034/K134). A full list of our Cas9 products can be found here:
https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html
|
| Is the protein preparation free of other nucleases? | |
|
We do not observe any nucleic acid degradation in the presence of Cas9 D10A Nickase.
|
| Does K132 contain a HIS-tag? | |
|
Yes, it does contain a His-tag.
|
| Does this cas9 nickase protein contain a Nuclear Localization Signal? | |
|
No, this Cas9 nickase protein does not have a Nuclear Localization Signal. If NLS is desired, we would recommend our Cas9 Nickase D10A NLS Protein (Cat#K034/K134). A full list of our Cas9 products can be found here:
https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html
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This product has no review yet.
Controls and Related Product:
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