Adeno CMV Null Adenovirus

  • Product Name

    Adeno CMV Null Adenovirus
  • Unit

    1.0 ml
  • Description

    The CMV null adenovirus is part of abm’s Adenoviral Expression System, and is used as a negative control for adenovirus applications. This packaged adenovirus contains a CMV promoter but without any gene insert. It will not express any recombinant protein. This adenovirus can be used to amplify more adenovirus by transducing HEK293 cells. For enhanced transduction efficiency, the use of ViralEntry™ Transduction Enhancer (G515) at 1:100 is recommended at the time of transduction.

  • Vector Map

    pAdeno (click blue link to view)
  • Titer

    106 pfu/mL
  • Storage Condition

    DMEM with 10% glycerol

  • Disclaimer

    Xiao, H. "Integration of cAMP and Ca2+ signaling pathways: Formation of a PDE1C and TrpC1 containing complex." Thesis : (2012). Application: Transduction.
    Wikstrom, JD et al. "AMPK regulates ER morphology and function in stressed pancreatic β-cells via phosphorylation of DRP1." Mol Endocrinol 27(10):1706-23 (2013). DOI: 10.1210/me.2013-1109. PubMed: 23979843 . Application: Transduction.
    Shultz, JC et al. "The Proto-oncogene PKCι regulates the alternative splicing of Bcl-x pre-mRNA." Mol Cancer Res. 10(5):660-9 (2012). DOI: 10.1158/1541-7786.MCR-11-0363. PubMed: 22522453 . Application: Transduction.
    McAllister, JM et al. "Overexpression of a DENND1A isoform produces a polycystic ovary syndrome theca phenotype." Proc Natl Acad Sci U S A 111(15):519-27 (2014). DOI: 10.1073/pnas.1400574111. PubMed: 24706793 . Application: Transduction.
    Imbert-Fernandez, Y et al. "Estradiol stimulates glucose metabolism via 6-phosphofructo-2-kinase (PFKFB3)." J Biol Chem. 289(13):9440-8 (2014). DOI: 10.1074/jbc.M113.539908. PubMed: 24515104 . Application: Transduction.
    Nakayama, H et al. "Regulation of mTOR Signaling by Semaphorin 3F-Neuropilin 2 InteractionsIn Vitro and In Vivo" Sci. Rep. 5:11789 (2015). DOI: 10.1038/srep11789. PubMed: 26156437 .
    Bernardo, NB et al. "The p21-activated kinase, PAK2, is important in the activation of numerous pancreatic acinar cell signaling cascades and in the onset of early pancreatitis events" BBA - Molecular Basis of Disease 1862.6:1122-1136 (2016). DOI: 10.1016/j.bbadis.2016.02.008. Application: Infection.
    Zhang, M., Wang, B., Urabe, G., Huang, Y., Plutzky, J., Kent, K. C., & Guo, L.-W. "The BD2 domain of BRD4 is a determinant in EndoMT and vein graft neointima formation" Cellular Signalling 61:20–29 (2019). DOI: 10.1016/j.cellsig.2019.05.005.
    Wen, W., Wang, Y., Li, H., Hu, D., Zhang, Z., Lin, H., & Luo, J. (2023). Upregulation of mesencephalic astrocyte‐derived neurotrophic factor MANF expression offers protection against alcohol neurotoxicity. Journal of Neurochemistry, 166(6), 943–959. Molecular Oncology. https://doi.org/10.1111/jnc.15921
    Waterbury, J. S., Teves, M. E., Gaynor, A., Han, A. X., Mavodza, G., Newell, J., Strauss, J. F., & McAllister, J. M. (2022). The PCOS GWAS Candidate Gene ZNF217 Influences Theca Cell Expression of DENND1A.V2, CYP17A1, and Androgen Production. Journal of the Endocrine Society, 6(7). https://doi.org/10.1210/jendso/bvac078
    Wang, Y., Wen, W., Li, H., Xu, H., Xu, M., Ma, M., & Luo, J. (2022). Deficiency of mesencephalic astrocyte-derived neurotrophic factor affects neurogenesis in mouse brain. Brain research bulletin, 183, 49-56. https://doi.org/10.1016/j.brainresbull.2022.02.019
    Wu, H., Li, H., Wen, W., Wang, Y., Xu, H., Xu, M., Frank, J. A., Wei, W., & Luo, J. (2021). MANF protects pancreatic acinar cells against alcohol‐induced endoplasmic reticulum stress and cellular injury. Journal of Hepato-Biliary-Pancreatic Sciences, 28(10), 883–892. https://doi.org/10.1002/jhbp.928
    ABM community
    Verified customer
    Asked on Feb 28 2025
    Answer
    abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
    ABM Scientific Support
    Answered on Feb 28 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.

    MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The concentration must be optimized for each cell type.  Typical selection amounts are around 0.1 - 0.5µg per ml.
    ABM Scientific Support
    Answered on Mar 24 2025
    Answer
    100mm is in fact about 5 X 60mm in area. 30ul to 200ul all should be fine and should not make much difference in the amplification of adenovirus.
    ABM Scientific Support
    Answered on Jan 01 1970
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
    ABM Scientific Support
    Answered on Mar 24 2025
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Current vector selected:
Adeno CMV Null Adenovirus
Cat. No.
000047A
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
Empty
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Current vector selected:
Cat. No.
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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