pLenti-III-mir-GFP Control Vector

  • Product Name

    pLenti-III-mir-GFP Control Vector
  • Unit

    500 ng
  • Description

    An insert empty version of our miRNA Expression Vector to use as a control during your miRNA experiments.

  • Vector Map

    pLenti-III-mir-GFP-Blank (click blue link to view)
  • Storage Condition

    Store at -20°C or below upon receipt

  • Disclaimer

    Xin, H et al. " MiR-133b Promotes Neural Plasticity and Functional Recovery After Treatment of Stroke with Multipotent Mesenchymal Stromal Cells in Rats Via Transfer of Exosome-Enriched Extracellular Particles" STEM CELLS 31:2737-2746 (2013). DOI: 10.1002/stem.1409. Application: miRNA control vector.
    He, Q;Zhou, X;Li, S;Jin, Y;Chen, Z;Chen, D;Cai, Y;Liu, Z;Zhao, T;Wang, A;, et al. "MicroRNA-181a suppresses salivary adenoid cystic carcinoma metastasis by targeting MAPK-Snai2 pathway" Biochim. Biophys. Acta 1830-11:5258-66 (2013). PubMed: 23911747.
    Bibikova, E et al. "Identification of Novel Pathways in the Pathogenesis of Diamond-Blackfan Anemia" Thesis : (0). Application: miRNA Expression Control.
    Sun, Y et al. "Reduced miR-3127-5p expression promotes NSCLC proliferation/invasion and contributes to dasatinib sensitivity via the c-Abl/Ras/ERK pathway" Sci. Rep 4:6527 (2014). DOI: 10.1038/srep06527. PubMed: 25284075. Application: Control Vector.
    Chopp, M et al. "MiR-133b Promotes Neural Plasticity and Functional Recovery After Treatment of Stroke with Multipotent Mesenchymal Stromal Cells in Rats Via Transfer of Exosome-Enriched Extracellular Particles" AlphaMed Press 12:2737-2746 (2013). DOI: 10.1002/stem.1409. Application: microRNA.
    Zhao, G et al. ""miR-203 Functions as a Tumor Suppressor by Inhibiting Epithelial to Mesenchymal Transition in Ovarian Cancer"" J Cancer Sci Thr 2:34-43 (2015). DOI: 10.4172/1948-5956.1000322.
    Jafarian, A et al. "The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9" PLoS One 6: (2015). DOI: 10.1371/journal.pone.0128650. PubMed: 26047014.
    Sendi, H et al. "miR-122 decreases HCV entry into hepatocytes through binding to the 3′ UTR of OCLN mRNA" Liver Int 6:1315-1323 (2015). DOI: 10.1111/liv.12698.
    Pajoohesh, M et al. "MicroRNA-145-based differentiation of human mesenchymal stem cells to smooth muscle cells" Biotechnol. Lett 38.11:1975 (2016). DOI: 10.1007/s10529-016-2177-1. Application: Lentiviral production.
    Ding, et al. "MicroRNA-585 acts as a tumor suppressor in non-small-cell lung cancer by targeting hSMG-1" Clinical and Translational Oncology 2016:1 (2016). DOI: 10.1007/s12094-016-1562-5. Application: Cloning.
    Kobayashi, M., Benakis, C., Anderson, C., Moore, M. J., Poon, C., Uekawa, K., … Darnell, R. B. "AGO CLIP Reveals an Activated Network for Acute Regulation of Brain Glutamate Homeostasis in Ischemic Stroke" Cell Reports 28(4):979–991.e6 (2019). DOI: 10.1016/j.celrep.2019.06.075.
    Lu., Yaoyong., . "“MicroRNA‐224, Negatively Regulated by c‐Jun, Inhibits Growth and Epithelial‐to‐Mesenchymal Transition Phenotype via Targeting ADAM17 in Oral Squamous Cell Carcinoma”" Journal of Cellular and Molecular Medicine vol. 23:no. 8 (2019). DOI: 10.1111/jcmm.14107..
    Zhang, H., Jiang, S., Guo, L., & Li, X. "MicroRNA‐1258, regulated by c‐Myb, inhibits growth and epithelial‐to‐mesenchymal transition phenotype via targeting SP1 in oral squamous cell carcinoma" Journal of Cellular and Molecular Medicine 23(4):2813–2821 (2019). DOI: 10.1111/jcmm.14189.
    Proaño-Pérez, E., Ollé, L., Guo, Y., Aparicio, C., Guerrero, M., Muñoz-Cano, R., & Martin, M. (2023). MITF Downregulation Induces Death in Human Mast Cell Leukemia Cells and Impairs IgE-Dependent Degranulation. International Journal of Molecular Sciences, 24(4), 3515. https://doi.org/10.3390/ijms24043515
    Patel, M., Wang, Y., Bartom, E. T., Dhir, R., Nephew, K. P., Matei, D., Murmann, A., Lengyel, E., & Peter, M. (2021). The balance between toxic versus nontoxic microRNAs determines platinum sensitivity in ovarian cancer. Cancer Research, 81(15), 3985–4000. https://doi.org/10.1158/0008-5472.CAN-21-0953
    Poudel, S., Napit, P. R., Briski, K. P., & Mattheolabakis, G. (2021). Oral Delivery of Nucleic Acids with Passive and Active Targeting to the Intestinal Tissue Using Polymer-Based Nanocarriers. Pharmaceutics, 13(7), 1075. https://doi.org/10.3390/pharmaceutics13071075
    Chatterjee, N., Espinosa-Diez, C., & Anand, S. (2020). A miR-494 dependent feedback loop regulates ER stress. BioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2020.05.12.088856
    Lu, Y., Huang, W., Chen, H., Wei, H., Luo, A., Xia Guang-sheng, Deng, X., & Zhang, G. (2019). MicroRNA‐224, negatively regulated by c‐jun, inhibits growth and epithelial‐to‐mesenchymal transition phenotype via targeting ADAM17 in oral squamous cell carcinoma. Journal of Cellular and Molecular Medicine, 23(8), 4913–4920. https://doi.org/10.1111/jcmm.14107
    Zhang, H., Jiang, S., Guo, L., & Li, X. (2019). MicroRNA‐1258, regulated by c‐Myb, inhibits growth and epithelial‐to‐mesenchymal transition phenotype via targeting SP1 in oral squamous cell carcinoma. Journal of Cellular and Molecular Medicine, 23(4), 2813–2821. https://doi.org/10.1111/jcmm.14189
    Li, W.-Y., Zhang, W.-T., Cheng, Y.-X., Liu, Y.-C., Zhai, F.-G., Sun, P., Li, H.-T., Deng, L.-X., Zhu, X.-F., & Wang, Y. (2018). Inhibition of KLF7-Targeting MicroRNA 146b Promotes Sciatic Nerve Regeneration. Neuroscience Bulletin, 34(3), 419–437. https://doi.org/10.1007/s12264-018-0206-x
    ABM community
    Verified customer
    Asked on Feb 28 2025
    Answer
    abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
    ABM Scientific Support
    Answered on Feb 28 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.

    MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The concentration must be optimized for each cell type.  Typical selection amounts are around 0.1 - 0.5µg per ml.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
    ABM Scientific Support
    Answered on Mar 24 2025
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Current vector selected:
pLenti-III-mir-GFP Control Vector
Cat. No.
m001
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
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DNAfectin™ Plus Transfection Reagent
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2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
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Empty
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Current vector selected:
Cat. No.
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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