saCas9 Nuclease NLS Protein (Lyophilized)

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The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).\u003C\/p\u003E\n\u003Cp\u003EThe Cas9 nuclease from the bacteria \u003Cem\u003EStaphylococcus aureus\u003C\/em\u003E, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5\u0026rsquo;-NNGRRN (preferably 5\u0026rsquo;-NNGRRT). saCas9 Nuclease NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. This saCas9 Nuclease NLS protein is offered as lyophilized powder for better long term storage and ambient temperature shipping. The protein is lyophilized as a low-salt powder, so it can be resuspended in your desired buffer for downstream applications.\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; width: 100%; height: 50px; background-color: #f9eae4;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022height: 89px;\u0022 valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022width: 91.0861%; height: 59px;\u0022\u003E\n\u003Cp style=\u0022text-align: center;\u0022\u003E\u003Cspan style=\u0022font-size: 14pt;\u0022\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a variety of Cas9 proteins. View our whole collection \u003Ca href=\u0022\/cas9-proteins.html\u0022\u003Ehere\u003C\/a\u003E.\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":"210","concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":"Lyophilized powder. Enzyme supplied with 10X Reaction Buffer.","including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"\/s\/a\/sacas9-nuclease-01_4.jpg","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":"After resuspension in 25 \u03bcl nuclease-free dH2O:\n20mM HEPES, pH 7.5, 100mM KCl, 1mM DTT, 0.1mM EDTA, 1% sucrose.","caution":"This product is distributed for laboratory research only. Not for diagnostic use.","storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":"Extra Freezer 1 door","note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":"Nuclease","cas_origin":"saCas9","cas_protein_marker":"No GFP","source":null,"endotoxin_level":"\u003C1.0 EU\/\u03bcg of recombinant protein as determined by the LAL method.","additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-22 08:56:41","updated_at":"2024-02-29 23:03:28","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379452,"parent_id":1911,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/s\/a\/sacas9-nuclease-01_4.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18084475,"sku_in_m2":"K152","value_id_m2":49909663,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:39:28"},{"id":385036,"parent_id":1911,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/cIeTtYGF9S7htvIFUHHaw1sU4xE2iASMdFN9Jgi0.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-11-18 22:31:48","updated_at":"2024-11-18 22:31:52"}],"gene":null}