Scrambled siRNA GFP Lentivector

    Jin, Q;Liu, G;Domeier, PP;Ding, W;Mulder, KM;, et al. "Decreased tumor progression and invasion by a novel anti-cell motility target for human colorectal carcinoma cells" PLoS ONE 8-6:e66439 (2013). DOI: 10.1371. PubMed: 23755307.
    Liang, Q;Dexheimer, TS;Zhang, P;Rosenthal, AS;Villamil, MA;You, C;Zhang, Q;Chen, J;Ott, CA;Sun, H;Luci, DK;Yuan, B;Simeonov, A;Jadhav, A;Xiao, H;Wang, Y;Maloney, DJ;Zhuang, Z;, et al. "A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses" Nat. Chem. Biol. 10-4:298-304 (2014). PubMed: 24531842.
    Bayazitov, IT et al. "Forward suppression in the auditory cortex is caused by the Ca(v)3.1 calcium channel-mediated switch from bursting to tonic firing at thalamocortical projections" J. Neurosci. 33:18940 - 50 (2013). DOI: 10.1523/JNEUROSCI.3335-13.2013. PubMed: 24285899. Application: siRNA.
    Yang, Z et al. "Screening with a Novel Cell-Based Assay for TAZ Activators Identifies a Compound That Enhances Myogenesis in C2C12 Cells and Facilitates Muscle Repair in a Muscle Injury Model" Mol Cell Biol. 34 (9):1607-1621 (2014). DOI: 10.1128/MCB.01346-13. PubMed: 24550007. Application: siRNA Control.
    Lellahi, SM. "POU3f2 in human gliomas - Expression pattern and functional role" Bergen Open Research Archive Thesis: (2014).
    Wang, B et al. "BET Bromodomain Blockade Mitigates Intimal Hyperplasia in Rat Carotid Arteries" J. Ebiom 2(11):1650-1661 (2015). DOI: 10.1016/j.ebiom.2015.09.045.
    Liu, XB et al. "Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression" Int. J. Clin. Exp. Path. 8(9):10887-10893 (2015). PubMed: 26617803.
    Yang, Z et al. "MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation" Int. J. Mol. Med. 3:579-586 (2015). DOI: 10.3892/ijmm.2014.2051. PubMed: PMC4314422.
    Chen, Y et al. "IL-6 signaling promotes DNA repair and prevents apoptosis in CD133+ stem-like cells of lung cancer after radiation" Radiat Oncol 227: (2015). DOI: 10.1186/s13014-015-0534-1. PubMed: PMC4647293.
    Shakya, A et al. "Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion." Mol Cell Biol 6:1014-25 (2015). DOI: 10.1128/MCB.01105-14. PubMed: 25582194 .
    Qu, J et al. "Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells" Scientific Reports 5: (2015). DOI: 10.1038/srep18491. Application: siRNA.
    Shimizu, T et al. "Microglia-Induced Activation of Noncanonical Wnt Signaling Aggravates Neurodegeneration in Demyelinating Disorders" Mol. Cell. Biol. 36.21:2728-2741 (2016). DOI: 10.1128/MCB.00139-16. Application: Research.
    Devaraju, P et al. "Haploinsufficiency of the 22q11.2 microdeletion gene Mrpl40 disrupts short-term synaptic plasticity and working memory through dysregulation of mitochondrial calcium" Mol. Psychiatry : (2016). DOI: 10.1038/mp.2016.75. Application: siRNA.
    Rong, H et al. ""Notch signaling suppresses regulatory T cell function in murine experimental autoimmune uveitis "" Immunology 149.4:447-459 (2016). DOI: 10.1111/imm.12663.
    Pandya, et al. "Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice" Mol. Psychiatry 2016: (2016). DOI: 10.1038/mp.2016.145. Application: Lentivirus Production.
    Zhang, et al. "The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line" Tumour Biology 37.5:5847 (2016). DOI: 10.1007/s13277-015-4405-z. Application: Generation of lentiviral vectors.
    Wu, L., Wang, L., & Chai, X. (2023). Interleukin-17 receptor C is essential for the pro-inflammatory pathogenicity of granulocyte-macrophage-colony-stimulating factor-producing T helper cells in experimental autoimmune uveitis. Cellular Immunology, 390, 104740. https://doi.org/10.1016/j.cellimm.2023.104740
    Wang, Y.-J., Di, X.-J., Zhang, P.-P., Chen, X., Williams, M. P., Han, D.-Y., Nashmi, R., Henderson, B. J., Moss, F. J., & Mu, T.-W. (2024). Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum. ELife, 13. CLOCKSS. https://doi.org/10.7554/elife.84798
    Park, S.-Y., Jung, S.-R., Kim, J.-Y., Kim, Y.-W., Sung, H.-K., Park, S.-Y., Doh, K.-O., & Koh, J.-H. (2024). Lactate promotes fatty acid oxidation by the tricarboxylic acid cycle and mitochondrial respiration in muscles of obese mice. American Journal of Physiology-Cell Physiology, 327(3), C619–C633. https://doi.org/10.1152/ajpcell.00060.2024
    Xiong, X., Yan, Z., Yan, L., Yang, X., Li, D., & Lin, G. (2024). Oxidized low‐density lipoproteins impair the pro‐atherosclerotic effect of granulocyte‐macrophage‐colony‐stimulating factor‐producing T helper cells on macrophages. Scandinavian Journal of Immunology, 99(5). https://doi.org/10.1111/sji.13362
    Wang, Y.-J., Di, X.-J., Han, D.-Y., Nashmi, R., Henderson, B. J., Moss, F. J., & Mu, T.-W. (2022). Hsp47 Promotes Biogenesis of Multi-subunit Neuroreceptors in the Endoplasmic Reticulum. https://doi.org/10.1101/2022.10.24.513629
    Kim, Y. A., Siddiqui, T., Blaze, J., Cosacak, M. I., Winters, T., Kumar, A., Tein, E., Sproul, A. A., Teich, A. F., Bartolini, F., Akbarian, S., Kizil, C., Hargus, G., & Santa-Maria, I. (2022). RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation. Acta neuropathologica, 145(1), 29–48. https://doi.org/10.1007/s00401-022-02511-7
    Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., Lee, S. O., & Chen, Y. (2019). NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer. Experimental Cell Research, 374(1), 181–188. https://doi.org/10.1016/j.yexcr.2018.11.022
    Bruno, N. E., Nwachukwu, J. C., Srinivasan, S., Nettles, C. C., Izard, T., Jin, Z., ... & Nettles, K. W. (2021). Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling. Nature Chemical Biology, 17(3), 307-316. https://doi.org/10.1038/s41589-020-00719-w
    Qin, Y.-J., Lin, T.-Y., Lin, X.-L., Liu, Y., Zhao, W.-T., Li, X.-Y., Lian, M., Chen, H.-W., Li, Y.-L., Zhang, X.-L., Xiao, D., Jia, J.-S., & Sun, Y. (2020). Loss of PDK4 expression promotes proliferation, tumorigenicity, motility and invasion of hepatocellular carcinoma cells. Journal of Cancer, 11(15), 4397–4405. https://doi.org/10.7150/jca.43459
    Liu, Z.-Z., Hong, C.-G., Hu, W.-B., Chen, M.-L., Duan, R., Li, H.-M., Yue, T., Cao, J., Wang, Z.-X., Chen, C.-Y., Hu, X.-K., Wu, B., Liu, H.-M., Tan, Y.-J., Liu, J.-H., Luo, Z.-W., Zhang, Y., Rao, S.-S., Luo, M.-J., & Yin, H. (2021). Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy, 17(10), 2766–2782. https://doi.org/10.1080/15548627.2020.1839286
    ABM community
    Verified customer
    Asked on Feb 28 2025
    Answer
    abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
    ABM Scientific Support
    Answered on Feb 28 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.

    MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The concentration must be optimized for each cell type.  Typical selection amounts are around 0.1 - 0.5µg per ml.
    ABM Scientific Support
    Answered on Mar 24 2025
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    Verified customer
    Asked on Mar 24 2025
    Answer
    The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
    ABM Scientific Support
    Answered on Mar 24 2025
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Current vector selected:
Scrambled siRNA GFP Lentivector
Cat. No.
LV015-G
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
Empty
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Current vector selected:
Cat. No.
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
Empty
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