BlasTaq™ 2X qPCR MasterMix
Cat. No.
G891
Unit
500 rxn (4 x 1.25 ml)
Price
$139.00
| Cat. No. | G891 | |||||||||
| Name | BlasTaq™ 2X qPCR MasterMix | |||||||||
| Unit | 500 rxn (4 x 1.25 ml) | |||||||||
| Category | qPCR Mastermixes | |||||||||
| Description |
BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains BlasTaq™ DNA Polymerase, a next-generation Taq polymerase strategically engineered by abm to deliver faster extension rates and stronger overall performance. With optimized reaction conditions, BlasTaq™ DNA Polymerase offers significantly improved processivity, higher yields, and enhanced sensitivity. These improvements allow for faster reaction times, shortening qPCR runs by up to 70% compared to reactions using wild-type Taq polymerase. BlasTaq™ possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but lacks 3’-5’ exonuclease activity. It also produces 3’-dA-tailed amplicons, making qPCR products generated with BlasTaq™ compatible with TA cloning systems. The MasterMix contains a fluorescent dye compatible with commonly used detection systems, such as SYBR Green™ and EvaGreen™. Additionally, ROX Reference Dye is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the ROX Machine Compatibility Chart. Product Features:
For a larger size, see Cat. No. G892 (2,500 rxn, 25.0 ml)
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| Concentration | 2X | |||||||||
| Storage Condition |
Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G891 |
Datasheet
| I still have additional questions, who do I contact? | |
|
For further details, please email technical@abmgood.com or 1-866-757-2414.
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| Is the dye set-up similar to SYBR Green™ & EvaGreen™? | |
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Yes, it is comparable.
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| What are the advantages to using BlasTaq™ 2X qPCR MasterMix? | |
|
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
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| How does BlasTaq™ differ from regular Taq polymerase? | |
|
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Does this have ROX reference dye? | |
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abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
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Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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| What is the recommended amount of template DNA to use in each reaction? | |
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Typically, 100 ng of genomic DNA is used as template in a 20 μl reaction, but the volume can be adjusted depending on the application.
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| Can I use BlasTaq™ 2X qPCR MasterMix for miRNA cDNA templates? | |
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Yes, BlasTaq™ 2X qPCR MasterMix can be used with miRNA cDNA templates and other appropriate applications, using the standard thermocycling conditions.
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| What is the difference between probe-based and dye-based qPCR assays? | |
|
Probe-based assays, like those using TaqMan probes, rely on a specific probe that binds to the target DNA sequence and emits a fluorescent signal when cleaved during amplification. This provides high specificity for detecting the target sequence. Dye-based assays, like those using SYBR Green or EvaGreen, rely on a dye that binds to double-stranded DNA, emitting fluorescence as the amount of DNA increases during amplification. Dye-based assays are simpler but may be less specific since the dye binds to any double-stranded DNA, not just the target sequence. BlasTaq™ Probe 2X qPCR MasterMix is specifically designed for probe-based assays, offering higher specificity, while dye-based assays use MasterMixes like BlasTaq™ 2X qPCR MasterMix (Cat. No. G891). |
- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
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Chen, L., Qin, Z., & Ruan, Z. B. (2023). Hyperoside alleviates doxorubicin-induced myocardial cells apoptosis by inhibiting the apoptosis signal-regulating kinase 1/p38 pathway. PeerJ, 11, e15315. https://doi.org/10.7717/peerj.15315
Guo, H., Chen, J., Yu, H., Dong, L., Yu, R., Li, Q., ... & Wang, W. (2023). Activation of Nrf2/ARE pathway by anisodamine (654-2) for inhibition of cellular aging and alleviation of radiation-induced lung injury. International Immunopharmacology, 124, 110864. https://doi.org/10.1016/j.intimp.2023.110864
Imtiaz, K., Farooqui, N., Ahmed, K., Zhamalbekova, A., Anwar, M. F., Nasir, A., ... & Abidi, S. H. (2025). Analysis of differential expression of matrix metalloproteinases and defensins in the nasopharyngeal milieu of mild and severe COVID-19 cases. PloS one, 20(2), e0304311. https://doi.org/10.1371/journal.pone.0304311
Jiang, K., Zhang, F., Chen, Y., Li, X., Zhao, X., Jiang, P., & Li, Y. (2024). Fosfenopril Attenuates Inflammatory Response in Diabetic Dry Eye Models by Inhibiting the TLR4/NF-κB/NLRP3 Signaling Pathway. Investigative Ophthalmology & Visual Science, 65(6), 2-2. https://doi.org/10.1167/iovs.65.6.2
Li, M., Liu, G., Jin, X. et al. Micropeptide MIAC inhibits the tumor progression by interacting with AQP2 and inhibiting EREG/EGFR signaling in renal cell carcinoma. Mol Cancer 21, 181 (2022). https://doi.org/10.1186/s12943-022-01654-1
Lin, H., Lin, J., Pan, T. et al. Polymeric immunoglobulin receptor deficiency exacerbates autoimmune hepatitis by inducing intestinal dysbiosis and barrier dysfunction. Cell Death Dis 14, 68 (2023). https://doi.org/10.1038/s41419-023-05589-3
Liu, X., Chen, Q., Yin, X., Wang, X., Ran, J., Yu, W., & Wang, B. (2024). Study on chromatin regulation patterns of expression vectors in the PhiC31 integration site. Epigenetics, 19(1), 2337085. https://doi.org/10.1080/15592294.2024.2337085
Nazim, F., Kayani, H. A., Ali Nathwani, A., Mir, F., & Abidi, S. H. (2022). CMV and EBV co-infection in HIV-infected children: infection rates and analysis of differential expression of cytokines in HIV mono-and HIV–CMV–EBV co-infected groups. Viruses, 14(8), 1823. https://doi.org/10.3390/v14081823
Ruiting, L. U. O., Huang, Y., Ruimin, B. A. I., Meng, L. I. U., Liang, S. U. N., Xiaofei, W. A. N. G., & Zheng, Y. (2024). ATP Citrate Lyase is a General Tumour Biomarker and Contributes to the Development of Cutaneous Squamous Cell Carcinoma. Acta Dermato-Venereologica, 104, 23805. https://doi.org/10.2340/actadv.v104.23805
Wang, X., Luo, X., Wang, Z., Wang, Y., Zhao, J., & Bian, L. (2023). Identification of cancer stemness and M2 macrophage-associated biomarkers in lung adenocarcinoma. Heliyon, 9(9). https://doi.org/10.1016/j.heliyon.2023.e19114
Zahid, W., Farooqui, N., Zahid, N., Ahmed, K., Anwar, M. F., Rizwan-ul-Hasan, S., … Abidi, S. H. (2023). Association of Interferon Lambda 3 and 4 Gene SNPs and Their Expression with COVID-19 Disease Severity: A Cross-Sectional Study. Infection and Drug Resistance, 16, 6619–6628. https://doi.org/10.2147/IDR.S422095
Zhang, W., Liu, J., Wang, Y. et al. Prenatal bisphenol A exposure causes sperm quality and functional defects via Leydig cell impairment and meiosis arrest in mice offspring. Sci Rep 15, 9810 (2025). https://doi.org/10.1038/s41598-025-93538-9
Zhang, X., Liu, Y., Gong, B., Wang, X., Liu, X., & Liu, F. (2025). PACAP alleviates the defective epididymis and sperm function in LPS-induced acute mice epididymitis. bioRxiv, 2025-02. https://doi.org/10.1101/2025.02.23.639790
Zhang, Y., Liu, Y., Teng, Z. et al. Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility. Biol Res 56, 47 (2023). https://doi.org/10.1186/s40659-023-00459-w
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No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022dye-qPCR\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003EDye-based MasterMix\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG891\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G891\u0022\u003E$135.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix-g892.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix-g892.html\u0022 rel=\u0022noopener\u0022\u003EG892\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E2,500 rxn (25.0 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G892\u0022\u003E$545.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022probe-qPCR\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003EProbe-based MasterMix\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; Probe 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG890\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G890\u0022\u003E$135.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022qpcr-kit\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003ERT-qPCR Kit\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-one-step-rt-qpcr.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; Probe One-Step RT-qPCR\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-one-step-rt-qpcr.html\u0022 rel=\u0022noopener\u0022\u003EG596\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G596\u0022\u003E$115.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr\u0022 id=\u0022qpcr\u0022 data-text=\u0022qPCR MasterMixes\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cb\u003EqPCR MasterMixes\u003C\/b\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EStreamline your qPCR with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s MasterMixes. Our specially formulated MasterMixes contain our next generation enzymes as well as all the ingredients you need premixed into an optimized format, significantly simplifying your workflow. Enjoy reduced handling errors, high reproducibility and excellent yields.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1.2em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/premixed-and-ready-to-use-icon.png\u0022 \/\u003E\n\u003Cp\u003EPremixed and ready-to-use\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/simple-streamlined-for-high-throughput-use-icon.png\u0022 \/\u003E\n\u003Cp\u003ESimple and streamlined for high-throughput use\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/reduce-handling-errors-icon.png\u0022 \/\u003E\n\u003Cp\u003EReduce handling errors\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/MasterMixes-Kits-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EReliable Detection\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/blastaq-2x-qpcr-mastermix.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlastaq\u0026trade; 2X qPCR MasterMix reliable quantifies low expression genes.\u003C\/strong\u003E Expression levels of 10 target genes in Human Colorectal Cancer Lines were quantified using Blastaq\u0026trade; 2X qPCR MasterMix (Cat. No. G891). Values were normalized to GAPDH. Data courtesy of Dr. Vivek Vaish from Pune University.\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u0026nbsp;\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EOutstanding Sensitivity\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/MasterMixes-Kits-data-11.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; Probe One-Step RT-qPCR has superb sensitivity and signal-to-noise ratio. \u003C\/strong\u003E 20 \u0026micro;l One-Step RT-qPCR reactions (20 ng, 2 ng, 200 pg, 20 pg, or 2 pg of RNA per reaction) were carried out with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s BlasTaq\u0026trade; Probe One-Step RT-qPCR (Cat. No. G596) for GAPDH amplification.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-page-left-link\u0022 id=\u0022free_sample\u0022 data-text=\u0022Request a Free Sample\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESimply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1600px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E \u003C!-- onload=\u0022window.parent.scrollTo(0,0)\u0022--\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-introduction\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/Resources_DNA-Polymerase-Variations.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to PCR\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"qPCR Mastermixes \u0026 Kits","meta_keywords":null,"meta_description":null,"deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":104,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":824,"cat_no_base":"G891","parent_id":102,"description":"\u003Cp\u003E\u003Cstrong\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/strong\u003E provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains \u003Cstrong\u003EBlasTaq\u0026trade; DNA Polymerase\u003C\/strong\u003E, a next-generation Taq polymerase strategically engineered by \u003Cstrong\u003Eabm\u003C\/strong\u003E to deliver faster extension rates and stronger overall performance.\u003C\/p\u003E\n\u003Cp\u003EWith optimized reaction conditions, \u003Cstrong\u003EBlasTaq\u0026trade; DNA Polymerase\u003C\/strong\u003E offers significantly \u003Cstrong\u003Eimproved processivity, higher yields, and enhanced sensitivity\u003C\/strong\u003E. These improvements allow for \u003Cstrong\u003Efaster reaction times\u003C\/strong\u003E, shortening qPCR runs by \u003Cstrong\u003Eup to 70%\u003C\/strong\u003E compared to reactions using wild-type Taq polymerase. BlasTaq\u0026trade; possesses 5\u0026rsquo;-3\u0026rsquo; polymerase activity and 5\u0026rsquo;-3\u0026rsquo; exonuclease activity, but lacks 3\u0026rsquo;-5\u0026rsquo; exonuclease activity. It also produces 3\u0026rsquo;-dA-tailed amplicons, making qPCR products generated with BlasTaq\u0026trade; compatible with TA cloning systems.\u003C\/p\u003E\n\u003Cp\u003EThe MasterMix contains a \u003Cstrong\u003Efluorescent dye\u003C\/strong\u003E compatible with commonly used detection systems, such as \u003Cstrong\u003ESYBR Green\u0026trade;\u003C\/strong\u003E and \u003Cstrong\u003EEvaGreen\u0026trade;\u003C\/strong\u003E. Additionally, \u003Cstrong\u003EROX Reference Dye\u003C\/strong\u003E is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the \u003Ca href=\u0022\/uploads\/document\/ROX%20Machine%20Compatibility%20List_v1.pdf\u0022\u003EROX Machine Compatibility Chart\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EEnable streamlined protocol in a simple reaction set-up\u003C\/li\u003E\n\u003Cli\u003EAllow accurate quantification of a variety of gene targets\u003C\/li\u003E\n\u003Cli\u003EReduce pipetting steps to minimize the risk of contamination\u003C\/li\u003E\n\u003Cli\u003ECompatible with most real-time PCR instruments\u003C\/li\u003E\n\u003Cli\u003EContains dye comparable to SYBR Green\u0026trade; and EvaGreen\u0026trade; \u003Cbr \/\u003E(\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-2x-qpcr-mastermix.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003EProbe-based qPCR Master Mix also available\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003EFor a larger size, see \u003Ca href=\u0022\/blastaq-2x-qpcr-mastermix-g892.html\u0022\u003ECat. No. G892\u003C\/a\u003E (2,500 rxn, 25.0 ml)\u003C\/p\u003E\n\u003Cp style=\u0022margin: 20px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E \u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EProduct Component\u003C\/th\u003E\n\u003Cth\u003EQuantity\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/td\u003E\n\u003Ctd\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EROX Reference Dye\u003C\/td\u003E\n\u003Ctd\u003E50 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #d6effa; border-radius: 10px; vertical-align: middle; margin-bottom: 20px;\u0022 border=\u00220\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022padding: 30px;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003ECustomer Testimonial\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0022Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols.\u0022\u003C\/p\u003E\n\u003Cp\u003E- Dr. Vivek Vaish, Pune University\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022vertical-align: middle;\u0022\u003E\n\u003Ctd style=\u0022text-align: left; vertical-align: middle;\u0022 width=\u002215%\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\u0022 alt=\u0022ISO 13485:2016 MDSAP Certified\u0022 width=\u002285%\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003EISO 13485:2016 MDSAP Certified\u003C\/strong\u003E\u003Cbr \/\u003EOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":"2X","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"no_selection","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp\u003EStore at -20 \u0026deg;C.\u0026nbsp;This product is stable for 2 years from the date of shipping if stored and handled properly.\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":"PCR-1A","note":"\u003Cp\u003EBlasTaq\u0026trade; 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\n\u003Cdiv\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EMACHINE TYPE\u003C\/th\u003E\n\u003Cth\u003EMACHINE\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EHigh ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026nbsp;\u0026reg; 7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u0026trade;, StepOne\u0026trade;, OpenArray, PRISM\u0026trade; Sequencing Detection Series\u003C\/li\u003E\n\u003Cli\u003EBiometra TOptical\u003C\/li\u003E\n\u003Cli\u003EFluidigm BioMark\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EWafergene SmartChip System\u003C\/li\u003E\n\u003Cli\u003ETianLong TL998 System\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ELow ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026reg; 7500, 7500 Fast, Viia\u0026trade; 7, QuantStudio, QuantStudio 3\/5\/6\/7\u003C\/li\u003E\n\u003Cli\u003EBioGene InSyte\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EIllumina Eco\u003C\/li\u003E\n\u003Cli\u003EAnalytikjena qTower Series\u003C\/li\u003E\n\u003Cli\u003EStratagene\u0026reg; Mx3000, Mx3005, Mx4000\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENo ROX machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EBioRad\u0026reg; CFX96, CFX384, Chromo4\u0026trade;, CFX Connect\u0026trade;, Opticon 2, MiniOpticon\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ERoche LightCycler\u0026reg; (480, 1536, Nano)\u003C\/li\u003E\n\u003Cli\u003EMJ Research Opticon\u0026trade;, Opticon\u0026trade; 2, Chromo\u0026reg; 4\u003C\/li\u003E\n\u003Cli\u003EEppendorf\u0026trade; Realplex 4\u003C\/li\u003E\n\u003Cli\u003EBioGene SynChron\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ECorbett Rotor-gene\u0026reg; (3000, 6200, 62H0, 6500, 65H0, 6600)\u003C\/li\u003E\n\u003Cli\u003EEppendorf Mastercycler\u0026reg; realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)\u003C\/li\u003E\n\u003Cli\u003ECepheid SmartCyler, GeneXpert\u003C\/li\u003E\n\u003Cli\u003EEnigma\u0026trade; ML\u003C\/li\u003E\n\u003Cli\u003EIdaho LightScanner\u0026reg; (24, 32), RapidCycler\u0026reg;2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS\u003C\/li\u003E\n\u003Cli\u003EQiagen Rotor-Gene\u0026trade; (Q, 6000)\u003C\/li\u003E\n\u003Cli\u003ETakara Dice\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EThermo Scientific PikoReal\u003C\/li\u003E\n\u003Cli\u003EDNA-Technology DT96, DTlite, DT-322\u003C\/li\u003E\n\u003Cli\u003EBioer LineGene (3310\/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)\u003C\/li\u003E\n\u003Cli\u003EBioneer Exicycler\u0026trade;\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E","recommend":"\u003Cstyle\u003E\n.orange-button {\nbackground-color: #e96225; \/* orange *\/\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\nwidth: 100%;\nline-height: 1em;\n}\n\n.blue-button {\nbackground-color: #1b7cef;\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\n}\n\n.table-style{\ntext-align: left;\nborder-bottom: 2px solid #f4f4f4;\n}\n\n.left{\nfloat: left;\nwidth: 48%;\n}\n\n.col-3 {\nfloat: left;\nwidth: 33%;\n}\n\n.col-3-right{\nfloat: right;\nwidth: 32%;\n}\n\n.right{\nfloat: right;\nwidth: 48%;\n}\n\n.clear{\nclear: both;\n}\n.spacing{\npadding-right: 2.5em;\n}\n\n.orange-border {\n border-radius: 0.75em;\n border: 4px solid #E96225;\n padding: 0.75em 2em 0.75em;\n}\n\n\n@media only screen and (max-width: 700px) {\n .left{\n width: 100%;\n }\n\n.right{\nwidth: 100%;\n}\n\n\n.col-3 {\nwidth: 100%;\n}\n\n.mobile{\nwidth: 100%;\n}\n\n.spacing{\npadding-right: 0;\n}\n}\n\u003C\/style\u003E\n\u003Cdiv class=\u0022left\u0022\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/pcr-buffet-program\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/pcr-buffet-product-page-banner.png\u0022 alt=\u0022PCR buffet\u0022 width=\u0022100%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022right\u0022\u003E\u003C\/div\u003E","depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":null,"updated_at":"2025-03-26 22:47:42","short_description":"\u003Cp\u003EBlasTaq\u003Csup\u003E\u2122\u003C\/sup\u003E\u00a02X qPCR MasterMix\u00a0provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains\u00a0abm\u2019s strategically-engineered, next generation Taq Polymerase, BlasTaq\u003Csup\u003ETM\u003C\/sup\u003E\u00a0DNA Polymerase, providing for\u00a0rapid extension rates and robust performance.\u00a0\u00a0\u003C\/p\u003E","reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":380640,"parent_id":102,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/g\/8\/g891-2-blastaq-2x-qpcr-mastermix_product-image.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18124172,"sku_in_m2":"G891","value_id_m2":49912360,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:39:19"},{"id":380644,"parent_id":102,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/g\/8\/g891-blastaq-qpcr-2x-mastermix-dr._vivek_vaish-watermarked.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":18124172,"sku_in_m2":"G891","value_id_m2":49912408,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2024-04-11 23:12:56"},{"id":384942,"parent_id":102,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/LCumfUTkHQwcNMRRFgWBTUa3y5CoaDg5x6h4C0MR.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-07-17 21:50:50","updated_at":"2024-07-17 21:54:04"},{"id":384943,"parent_id":102,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/8gZKmZ9W1JuswT5eXVmMb0RXKuNg0bFolvIMBIh6.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":4,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-07-17 21:50:55","updated_at":"2024-07-17 21:54:04"},{"id":384944,"parent_id":102,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/LS8O2j9hmGrDQXXBmjwyk9G5ouA06qVwx2FaaRXv.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":5,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-07-17 21:50:59","updated_at":"2024-07-17 21:54:04"}],"gene":null}