All-In-One 5X RT MasterMix with gDNA Removal

Cat. No.
G592
Unit
100 rxn
Price
$209.00

PCR buffet

Cat. No. G592
Name All-In-One 5X RT MasterMix with gDNA Removal
Unit 100 rxn
Category Reverse Transcriptase & RT-PCR
Description

The All-In-One 5X RT MasterMix with gDNA Removal offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, all in a single tube. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis.

The optimized MasterMix includes abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo (dT)s and Random Primers. By eliminating the need for separate reagents, it provides a straightforward, reliable, and reproducible cDNA synthesis process with high yields—even from difficult samples. The high-quality cDNA produced is suitable for a wide range of downstream applications.

Product Features:

  • Easy one-step setup reduces pipetting and sample handling
  • Removes contaminating gDNA with temperature-sensitive DNase
  • High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
  • Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G138)
  • Also includes dNTPs, Oligo (dT)s, and random primers
  • Included in abm's PCR Buffet Program

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Product Component Quantity
All-In-One 5X RT MasterMix 100 rxn (400 µl)
Nuclease-Free H2O 1.0 ml
ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Application
  • Generation of templates for use in RT-PCR and qRT-PCR
  • cDNA synthesis from ssRNA
  • cDNA library construction
  • Generation of probes for hybridization
  • DNA primer extension
Concentration 5X
Storage Condition

Store at -20°C.

Note

For applications using pure RNA where genomic DNA removal is not required, try our OneScript® Hot 5X RT MasterMix (Cat. No. G590). It offers a faster protocol for an even more efficient cDNA synthesis.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G592
Datasheet
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Supporting Protocol
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G590
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CAT.NO
G594
UNIT
100 rxn
PRICE
$207.00

CAT.NO
G236
UNIT
100 rxn
PRICE
$212.00

CAT.NO
G898
UNIT
20 rxn
PRICE
$185.00

CAT.NO
G597
UNIT
100 rxn
PRICE
$222.00

CAT.NO
G596
UNIT
100 rxn
PRICE
$118.00