Cas9 Nickase Lentiviral Vector
| Cat. No. | K005 |
| Name | Cas9 Nickase Lentiviral Vector |
| Unit | 10 µg |
| Description |
Lentiviral vector expressing Cas9 Nickase (D10A) with N-terminal SV40 NLS. This modified version of the Cas9 protein introduces single-strand breaks (nicks) in DNA instead of double-strand breaks, reducing off-target effects in genome editing. |
| Applications |
CRISPR Cas9 gene editing. |
| Vector | pLenti-EF1a-Cas9-Nickase-SV40-Puro |
| Storage Condition | -20°C or below. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K005 |
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| What kind of mutation does this contain? | |
|
D10A mutation
|
| Is this Cas9-nickase codon-optimized for a specific cell type? | |
|
It is codon optimized for human expression.
|
| What type of selection marker does this contain? | |
|
This lentiviral vector contains a puromycin marker for mammalian selection.
|
| What is the sequence? | |
|
We are unable to release the sequence of our Cas9 Nickase as it has been codon optimized and is proprietary information.
|
| Can you provide the sequence for this plasmid? | |
|
We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
|
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).
This product has no review yet.
Controls and Related Product:
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