CRISPRa dCas9-VPR Lentiviral Vector

Cat. No.
K097
Unit
10 µg
Price
$212.00
Cat. No. K097
Name CRISPRa dCas9-VPR Lentiviral Vector
Unit 10 µg
Description

This Cas9 Double Mutant (dCas9) carries the mutations D10A and H840A which inactivates both nuclease and nickase activities. Using a target-specific guide RNA (sgRNA), it can bind to a specific genomic position without inducing DNA cleavage. In this construct, dCas9 is fused to the tripartite complex VPR (VP64, p65 and Rta). These transcriptional activators work in tandem to recruit transcription factors which in turn up-regulate expression of your gene of interest. In comparison to dCas9-SAM system, dCas9-VPR does not require specialized MS2 sgRNAs and it allows for streamlined transfection or infection as the vector encodes a single fusion protein.

Vector pLenti-SFFV-dCas9-VPR
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K097
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