TO1-3PEG-Biotin Fluorophore
Cat. No.
G7955
Unit
250 µM (100 µl)
Price
$375.00
| Cat. No. | G7955 | ||
| Name | TO1-3PEG-Biotin Fluorophore | ||
| Unit | 250 µM (100 µl) | ||
| Category | RNA Tracking (RNA Mango) | ||
| Description |
RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including:
TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants are available. Learn more about the RNA Mango technology here.
Watch RNA Mango in Action Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.
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||
| Storage Condition | Stored at -20°C. Protect from light. | ||
| Depositor | Unrau Lab | ||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G7955 |
Datasheet
| Can I use water with my fluorophore dyes? | |
|
We strongly recommend to use DMF, DMSO, 10% Acetonitrile, or MeOH-CH₂ Cl₂ for stability. Do no store in water as it may break down the dye.
|
| Which aptamers should I use for each RNA Mango fluorophore? | |
|
Use our RNA Mango Aptamer Systems chart to help you select the appropriate aptamer and fluorophore combination for your research needs.
|
| Any tips for using RNA Mango aptamers in cellular transcripts? | |
|
We have an aptamer insertion guideline for cellular transcript, which can be found here.
|
| What is the difference between TO1-3PEG-Biotin (G955) and TO1-3PEG-Desthiobiotin (G956)? | |
|
TO1-3PEG-Biotin (G955) and TO1-3PEG-Desthiobiotin (G956) are quite similar in terms of binding and fluorescence. They are distinct in how they interact with streptavidin magnetic beads. Specifically, desthiobiotin can be displaced from them by the addition of free biotin while TO1-3PEG-Biotin cannot.
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- Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D'Amaré AR. et al. "Structural basis for high-affinity fluorophore binding and activation by RNA Mango." Nat Chem Biol. 2017 Jul;13(7):807-813. DOI: 10.1038/nchembio.2392.
- Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al. "Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers." RNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24. PubMed : 27777365.
- Ouellet J. et al. "RNA Fluorescence with Light-Up Aptamers." Front Chem. 2016 Jun 28;4:29. DOI: 10.3389/fchem.2016.00029. eCollection 2016. Review. PubMed : 27446908.
- Panchapakesan SS, Jeng SC, Unrau PJ. et al. "RNA complex purification using high-affinity fluorescent RNA aptamer tags." Ann N Y Acad Sci. 2015 Apr;1341:149-55. DOI: 10.1111/nyas.12663. Epub 2015 Jan 13.
- Dolgosheina EV, Jeng SC, Panchapakesan SS, Cojocaru R, Chen PS, Wilson PD, Hawkins N, Wiggins PA, Unrau PJ. et al. "RNA mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking." ACS Chem Biol. 2014 Oct 17;9(10):2412-20. DOI: 10.1021/cb500499x. Epub 2014 Aug 21.
- Autour, A., C Y Jeng, S., D Cawte, A., Abdolahzadeh, A., Galli, A., Panchapakesan, S. S. S., Rueda, D., Ryckelynck, M., & Unrau, P. J. (2018). Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells. Nature communications, 9(1), 656. https://doi.org/10.1038/s41467-018-02993-8
- Trachman, R. J., & Ferré-D'Amaré, A. R. (2019). Tracking RNA with light: selection, structure, and design of fluorescence turn-on RNA aptamers. Quarterly reviews of biophysics, 52, e8. https://doi.org/10.1017/S0033583519000064
- Trachman, R. J., 3rd, Autour, A., Jeng, S. C. Y., Abdolahzadeh, A., Andreoni, A., Cojocaru, R., Garipov, R., Dolgosheina, E. V., Knutson, J. R., Ryckelynck, M., Unrau, P. J., & Ferré-D'Amaré, A. R. (2019). Structure and functional reselection of the Mango-III fluorogenic RNA aptamer. Nature chemical biology, 15(5), 472–479. https://doi.org/10.1038/s41589-019-0267-9
- Kong, K. Y. S., Jeng, S. C. Y., Rayyan, B., & Unrau, P. J. (2021). RNA Peach and Mango: Orthogonal two-color fluorogenic aptamers distinguish nearly identical ligands. RNA (New York, N.Y.), 27(5), 604–615. Advance online publication. https://doi.org/10.1261/rna.078493.120
- Cawte, A. D., Unrau, P. J., & Rueda, D. S. (2020). Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Nature communications, 11(1), 1283. https://doi.org/10.1038/s41467-020-14932-7
- Panchapakesan, S. S. S., Ferguson, M. L., Hayden, E. J., Chen, X., Hoskins, A. A., & Unrau, P. J. (2017). Ribonucleoprotein purification and characterization using RNA Mango. RNA (New York, N.Y.), 23(10), 1592–1599. https://doi.org/10.1261/rna.062166.117
- Mitra, J., & Ha, T. (2019). Nanomechanics and co-transcriptional folding of Spinach and Mango. Nature communications, 10(1), 4318. https://doi.org/10.1038/s41467-019-12299-y
- Shi, J., Gao, X., Tian, T., Yu, Z., Gao, B., Wen, A., You, L., Chang, S., Zhang, X., Zhang, Y., & Feng, Y. (2019). Structural basis of Q-dependent transcription antitermination. Nature communications, 10(1), 2925. https://doi.org/10.1038/s41467-019-10958-8
- Fang, C., Philips, S. J., Wu, X., Chen, K., Shi, J., Shen, L., Xu, J., Feng, Y., O'Halloran, T. V., & Zhang, Y. (2021). CueR activates transcription through a DNA distortion mechanism. Nature chemical biology, 17(1), 57–64. https://doi.org/10.1038/s41589-020-00653-x
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One such researcher is \u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.sfu.ca\/mbb\/people\/faculty\/peter-unrau.html\u0022\u003EDr. Peter Unrau of Simon Fraser University\u003C\/a\u003E. He and his team have created RNA Mango, a novel technology with a number of useful applications.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 0px;\u0022\u003E\u003Cimg style=\u0022padding-bottom: 2em;\u0022 src=\u0022\/assets\/images\/wysiwyg\/dr-peter-unrau.jpg\u0022 width=\u0022500%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-md-12 col-sm-12 col-lg-12 col-xs-12\u0022\u003E\n\u003Cdiv class=\u0022mini-box2\u0022\u003E\u003Cspan class=\u0022bluetext\u0022\u003E\u003Cb\u003EDocuments\u003C\/b\u003E\u003C\/span\u003E\n\u003Cul style=\u0022padding-left: 20px;\u0022 class=\u0022grey-list grey-link\u0022\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/assets\/productdocument\/document\/a\/b\/abm_rna-mango-insertion-guidelines.pdf\u0022\u003ERNA Mango Insertion Guidelines\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli\u003E\u003Ca class=\u0022abm-category-list-cname\u0022 href=\u0022\/assets\/productdocument\/document\/a\/b\/abm_rna-mango-aptamer-systems_performance-data.pdf\u0022\u003ERNA Mango Binding, Fluorescence, and Resistance Properties\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section2\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022 style=\u0022padding: 0px 0px 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv id=\u0022abm-category-section2\u0022 class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable id=\u0022table2\u0022 class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to1-3peg-biotin-fluorophore-g7955.html\u0022\u003ETO1-3PEG-Biotin Fluorophore\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to1-3peg-biotin-fluorophore-g7955.html\u0022\u003EG7955\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E0.5 mg\/ml (500 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G955\u0022\u003E\u0026nbsp;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to1-3peg-desthiobiotin-fluorophore-g7956.html\u0022\u003ETO1-3PEG-Desthiobiotin Fluorophore\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to1-3peg-desthiobiotin-fluorophore-g7956.html\u0022\u003EG7956\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E0.5 mg\/ml (500 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G956\u0022\u003E\u0026nbsp;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to3-3peg-biotin-fluorophore-g7959.html\u0022\u003ETO3-3PEG-Biotin Fluorophore\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/to3-3peg-biotin-fluorophore-g7959.html\u0022\u003EG7959\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E0.5 mg\/ml (500 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G959\u0022\u003E\u0026nbsp;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/yo3-3peg-biotin-fluorophore-g7957.html\u0022\u003EYO3-3PEG-Biotin Fluorophore\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/yo3-3peg-biotin-fluorophore-g7957.html\u0022\u003EG7957\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E0.5 mg\/ml (500 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G957\u0022\u003E\u0026nbsp;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: white;\u0022 align=\u0022left;\u0022\u003E\n\u003Ctd style=\u0022text-align: left;\u0022 colspan=\u00224\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003E\u003Ca href=\u0022\/assets\/productdocument\/document\/a\/b\/abm_rna-mango-aptamer-systems_performance-data.pdf\u0022\u003E* Click here for data on binding affinity to Mango and Peach aptamers\u003C\/a\u003E\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section3\u0022 class=\u0022abm-container-fluid abm-page-left-link\u0022 data-text=\u0022Key Features\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EKey Features\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-md-12 col-sm-12 col-lg-12 col-xs-12\u0022\u003E\n\u003Cp\u003ERNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD \u0026asymp; 3nM) , and the strong ~1000X enhancement of the dye\u0026rsquo;s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100). \u003Cbr \/\u003E\u003Cbr \/\u003EThe Thizole Orange (TO) dye has a number of other desirable properties including:\u003C\/p\u003E\n\u003Cul style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cli\u003ESmall size\u003C\/li\u003E\n\u003Cli\u003ELack of toxicity\u003C\/li\u003E\n\u003Cli\u003EPlasma and nuclear membrane permeability\u003C\/li\u003E\n\u003Cli\u003EShort intracellular half-life\u003C\/li\u003E\n\u003Cli\u003EThe accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure\u003C\/li\u003E\n\u003Cli\u003EThe \u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=TO1\u0022\u003ETO1 and TO3 dyes\u003C\/a\u003E can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach aptamer systems (\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/rnajournal.cshlp.org\/content\/early\/2021\/03\/05\/rna.078493.120\u0022 rel=\u0022noopener\u0022\u003EKong et. al. 2021\u003C\/a\u003E)\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003ETO1-biotin is the standard variety of TO dye for RNA Mango experiments. \u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=fluorophore\u0026amp;fc_ids%5B%5D=1\u0026amp;fc_ids%5B%5D=16\u0022\u003EView\u003C\/a\u003E our complete list of RNA Mango dyes.\u003Cbr \/\u003E\u003Cbr \/\u003ECan\u0027t find what you\u0027re looking for, or have a specific request? \u003Cbr \/\u003ESpeak to one of our technical support specialists today \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto:technical@abmgood.com\u0022\u003Etechnical@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section4\u0022 class=\u0022abm-container-fluid abm-page-left-link\u0022 data-text=\u0022The RNA Mango Workflow\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EThe RNA Mango Workflow\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022 style=\u0022display: flex; flex-wrap: wrap;\u0022\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/r\/n\/RNA-1.jpg\u0022 alt=\u0022The RNA Mango Aptamer\u0022 \/\u003E\u003C\/a\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003EThe RNA Mango Aptamer\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EThe system has two components: the RNA Mango aptamer, and the TO-1 dye. The dye only fluoresces when bound to the Mango aptamer. \u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/r\/n\/RNA-2.jpg\u0022 alt=\u0022Tagging Your RNA\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ETagging Your RNA\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EFor mRNA, insert the tag into the 3\u0027UTR. For structured non-coding RNA, replace a stem-loop that is not essential to the RNA function.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/r\/n\/RNA-3.jpg\u0022 alt=\u0022Application: RNA Visualization\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003EApplication: RNA Visualization\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EExpress RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Soak cells in TO-1 Biotin dye to illuminate the localization of the RNA of interest.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/r\/n\/RNA-4.jpg\u0022 alt=\u0022pApplication: RNA-RNA or RNA-protein complex pulldown\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003EApplication: RNA-RNA or RNA-protein complex pulldown\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003EExpress RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Lyse the cells, and recover RNA of interest with bound RNA or proteins using streptavidin beads\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003ELaboratory Results\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/rna_visualization\/RNA-Mango_RNP-Pulldown-Purification_data-01.png\u0022 alt=\u0022Yeast U1 Ribonucleoprotein (RNP) Complex Pulldown Purification\u0022 \/\u003E\u003C\/a\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003EYeast U1 Ribonucleoprotein (RNP) Complex Pulldown Purification\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003ELeft: RNAs present in native extract in which U1M migrates as doublet. Right: U1M is a single band RNP present after mango-based purification using TO1 3PEG-Desthiobiotin Fluorophore \u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/category\/rna_visualization\/magno.png\u0022 alt=\u0022RNA Mango in a test tube\u0022 \/\u003E\u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ERNA Mango in a Test Tube\u003C\/strong\u003E \u003Cbr \/\u003E\u003Cspan\u003E Mango dye binding to \u003Ci\u003Ein vitro\u003C\/i\u003E transcribed RNA Mango-tagged sgRNA. Stable binding and fluorescence even after leaving the tube for 1 month at room temperature.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr style=\u0022border-top: 2px solid #bbb;\u0022 \/\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ciframe width=\u0022100%\u0022 height=\u0022300px\u0022 title=\u0022YouTube video player\u0022 src=\u0022https:\/\/www.youtube.com\/embed\/M1_8mYwuBeE\u0022 frameborder=\u00220\u0022 allowfullscreen=\u0022allowfullscreen\u0022\u003E\u003C\/iframe\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ERNA Mango in Action\u003C\/strong\u003E\u003Cbr \/\u003ETranscription reaction were carried out in 300 \u0026micro;L volumes using T7 RNA polymerase (400 U, 50U\/\u0026micro;L, applied biological materials), 0.5 \u0026micro;M TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U\/\u0026micro;L, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 \u0026micro;M DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca href=\u0022\/assets\/productdocument\/document\/a\/b\/abm_rna-mango-aptamer-systems_performance-data.pdf\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/RNA-Mango-binding-fluorescence-resistance.png\u0022 alt=\u0022RNA Mango Binding, Fluorescence, and Resistance Properties\u0022 width=\u0022748\u0022 height=\u0022559\u0022 \/\u003E\u003C\/a\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003E\u003Cstrong\u003ERNA Mango Binding, Fluorescence, and Resistance Properties\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cspan\u003E Includes extensive citations list.\u003C\/span\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr style=\u0022border-top: 2px solid #bbb;\u0022 \/\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cul class=\u0022htmlcontent-home\u0022\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/TO1-TO3-2-color-transcription-reporter-assay-rna-mango-rna-peach-aptamers-1.png\u0022 alt=\u0022TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach systems\u0022 width=\u0022654\u0022 height=\u00221200\u0022 \/\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-6 col-md-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/TO1-TO3-2-color-transcription-reporter-assay-rna-mango-rna-peach-aptamers-2.png\u0022 alt=\u0022TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach systems\u0022 width=\u0022654\u0022 height=\u00221200\u0022 \/\u003E\u003C\/a\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-sub-category-image-title\u0022\u003ETO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach aptamer systems (\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/rnajournal.cshlp.org\/content\/early\/2021\/03\/05\/rna.078493.120\u0022 rel=\u0022noopener\u0022\u003EKong et. al. 2021\u003C\/a\u003E)\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv id=\u0022abm-category-section5\u0022 class=\u0022abm-container-fluid abm-page-left-link\u0022 data-text=\u0022Top Publications\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003ETop Publications\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module col-md-12\u0022 style=\u0022padding-left: 0; padding-right: 30px;\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1261\/rna.062166.117\u0022 rel=\u0022noopener\u0022\u003E01\u003C\/a\u003E\u003C\/span\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003ERibonucleoprotein purification and characterization using RNA Mango. \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003EPanchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al. \u003Cbr \/\u003ERNA. 2017 Oct;23(10):1592-1599. \u003C\/i\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1261\/rna.062166.117\u0022 rel=\u0022noopener\u0022\u003EDOI: 10.1261\/rna.062166.117. PubMed: 28747322. PubMed Central PMCID: PMC5602116.\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/nchembio.2392\u0022 rel=\u0022noopener\u0022\u003E02\u003C\/a\u003E\u003C\/span\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003EStructural basis for high-affinity fluorophore binding and activation by RNA Mango. \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003ETrachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferr\u0026eacute;-D\u0027Amar\u0026eacute; AR. et al. \u003Cbr \/\u003ENat Chem Biol. 2017 Jul;13(7):807-813. \u003C\/i\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/nchembio.2392\u0022 rel=\u0022noopener\u0022\u003EDOI: 10.1038\/nchembio.2392. Epub 2017 May 29. PubMed : 28553947.PubMed Central PMCID: PMC5550021.\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=27777365\u0022 rel=\u0022noopener\u0022\u003E03\u003C\/a\u003E\u003C\/span\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003EFluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers. \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003EJeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al\u003Cbr \/\u003ERNA. 2016 Dec;22(12):1884-1892. Epub 2016 Oct 24\u003C\/i\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=27777365\u0022 rel=\u0022noopener\u0022\u003EPubMed : 27777365. PubMed Central PMCID: PMC5113208.\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"RNA Mango","meta_keywords":"RNA Mango,RNA Mango aptamer TO-1,TO-1 Biotin,TO1-3PEG-Biotin Fluorophore,TO3-3PEG-Biotin Fluorophore,TO1-4PEG-Desthiobiotin Fluorophore ,TO3-4PEG-Desthiobiotin Fluorophore,Thiozol Orange,RNA fluorophore,RNA Spinach,RNA Spinach aptamer,DFHBI fluorophore,RNA visualization,RNA tracking,RNA live cell imaging,RNA pulldown,single-RNA tracking,RNA technology","meta_description":"The RNA Mango aptamer and thiozole orange dye together is the most advanced RNA tracking,visualization and pull down technology.","deleted_at":null,"enable":"Y","parent_list":"27","table_name":"abm_catalog_general","image":null,"independentPage":0,"top_type":1,"sort_order":109,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":18605,"cat_no_base":null,"parent_id":3429,"description":"\u003Cp\u003ERNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD \u0026asymp; 3nM) , and the strong ~1000X enhancement of the dye\u0026rsquo;s fluorescence when bound to the Mango apatmer (Fluorescent enhancement FE=1,100).The TO dye has a number of other desirable properties including:\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003Esmall size\u003C\/li\u003E\n\u003Cli\u003Elack of toxicity\u003C\/li\u003E\n\u003Cli\u003Eplasma and nuclear membrane permeability\u003C\/li\u003E\n\u003Cli\u003Eshort intracellular half-life\u003C\/li\u003E\n\u003Cli\u003Ethe accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003ETO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants are available. Learn more about the RNA Mango technology \u003Ca href=\u0022\/RNA-Mango.html\u0022\u003Ehere\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EWatch RNA Mango in Acti\u003C\/strong\u003E\u003Cstrong\u003Eon\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Ciframe title=\u0022YouTube video player\u0022 src=\u0022https:\/\/www.youtube.com\/embed\/M1_8mYwuBeE\u0022 width=\u0022660\u0022 height=\u0022372\u0022 frameborder=\u00220\u0022 allowfullscreen=\u0022allowfullscreen\u0022 data-mce-fragment=\u00221\u0022\u003E\u003C\/iframe\u003E\u003C\/p\u003E\n\u003Cp\u003ETranscription reaction were carried out in 300 \u0026micro;L volumes using T7 RNA polymerase (400 U, 50U\/\u0026micro;L, applied biological materials), 0.5 \u0026micro;M TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U\/\u0026micro;L, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 \u0026micro;M DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; border-radius: 10px; background-color: #e6f5fd; vertical-align: middle;\u0022 border=\u00220\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022padding: 30px;\u0022 width=\u002270%\u0022\u003E\u003Cstrong\u003EThe most advanced RNA tracking, visualization and pull down technology.\u003C\/strong\u003E\n\u003Cp\u003ETechnology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is \u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.rnabiochemistry.com\/\u0022\u003EDr. Peter Unrau of Simon Fraser University\u003C\/a\u003E. He and his team have created RNA Mango, a novel technology with a number of useful applications.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 30px;\u0022\u003E\u003Cimg src=\u0022https:\/\/info.abmgood.com\/assets\/img\/blog\/dr-peter-unrau.png\u0022 width=\u0022100%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"Stored at -20\u00b0C. Protect from light.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":"Media Team, Joey","stain_color":null,"supplier":"abm","internal_supplier":null,"internal_note":null,"inventory_location":null,"note":null,"recommend":null,"depositor":"Unrau Lab","licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2025-03-21 17:30:15","updated_at":"2025-03-25 18:44:36","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":388578,"parent_id":3429,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/pUEFgOzIBh1U6559uIajYdslbvvNFsRTikuyjeBz.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-21 17:29:29","updated_at":"2025-03-21 17:30:06"},{"id":388579,"parent_id":3429,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/PSC7h23PoYVfnuRv2eZsP8QCpt3ctZVxjU4rklAu.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-21 17:29:36","updated_at":"2025-03-21 17:30:06"},{"id":388580,"parent_id":3429,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/6jVse3c02GAyb5mkJKjqWKBOyw6rwVkR0f1WSqpa.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-21 17:29:44","updated_at":"2025-03-21 17:30:06"},{"id":388581,"parent_id":3429,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/19wVeg9jeSfqnLejCN0vBHtNDgQ5Wmr79tNtJwog.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":4,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-21 17:29:47","updated_at":"2025-03-21 17:30:06"},{"id":388582,"parent_id":3429,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/eyaNmv30eLNwSbCZGQ63yXVCr7W7A66MGpIgGq9s.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":5,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-21 17:29:51","updated_at":"2025-03-21 17:30:06"}],"gene":null}