T7 RNA Polymerase
Cat. No.
E041
Unit
100 ul (5000 U)
Price
$70.00
| Cat. No. | E041 | ||||||
| Name | T7 RNA Polymerase | ||||||
| Unit | 100 ul (5000 U) | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description |
T7 RNA Polymerase is a DNA-dependent enzyme that catalyzes the synthesis of RNA in the 5' to 3' direction. It works exclusively in the presence of its T7 phage promoter sequence, ensuring high specificity for transcription. This enzyme does not recognize SP6 or T3 RNA Polymerase promoter sequences, making it ideal for applications requiring precise and controlled RNA production from T7-driven templates.
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| Application |
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| Concentration | 50 U/ul | ||||||
| Components | Enzyme supplied with 10X Reaction Buffer | ||||||
| Storage Buffer | 50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM β-ME, 100 mM NaCl, 0.1% Triton®X-100 and 50% (v/v) Glycerol. | ||||||
| Storage Condition | Store all components at -20°C. | ||||||
| Note |
One unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume in 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E041 |
Datasheet
Search CoA here
| What is T7 RNA Polymerase used for? | |
|
T7 RNA Polymerase is used for synthesizing RNA transcripts, which can be used as hybridization probes, for in vitro translation, or for generating biologically active mRNA. It is also useful for creating large amounts of labelled or non-labelled RNA.
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| What is the optimal reaction condition for T7 RNA Polymerase? | |
|
The recommended reaction condition is to use 1X T7 RNA Polymerase Reaction Buffer and incubate the reaction at 37°C. Ensure the reaction mixture includes fresh dithiothreitol (DTT) and maintain the salt concentration below 50 mM for optimal enzyme activity.
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| How do I increase the yield of RNA in my reaction? | |
|
To increase RNA yield, you can increase the concentration of NTPs (up to 4 mM each) and adjust the magnesium concentration to 4 mM above the total NTP concentration.
|
| What is the definition of one unit of T7 RNA Polymerase? | |
|
One unit of T7 RNA Polymerase is defined as the amount required to incorporate 1 nmol ATP into acid-insoluble material in a 50 μl reaction volume over 1 hour at 37°C in 1X T7 RNA Polymerase Reaction Buffer.
|
| Why is DTT important for T7 RNA Polymerase activity? | |
|
Dithiothreitol (DTT) is essential for maintaining the enzyme’s activity. If you notice a decrease in activity, it may be due to the breakdown of DTT over time, even when stored at -20°C. In such cases, you should supplement the reaction with fresh DTT at a final concentration of 10 mM.
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Abdolahzadeh, A., Ang, Q. R., Caine, J. R., Panchapakesan, S. S. S., Thio, S., Cojocaru, R., & Unrau, P. J. (2024). Turn-on RNA Mango Beacons for trans-acting fluorogenic nucleic acid detection. RNA, 30(4), 392-403. https://doi.org/10.1261/rna.079833.123
Abdolahzadeh, A., Dolgosheina, E. V., & Unrau, P. J. (2019). RNA detection with high specificity and sensitivity using nested fluorogenic Mango NASBA. Rna, 25(12), 1806-1813. https://doi.org/10.1261/rna.072629.119
Arthur, S. E., Gao, J., Healy, S., Rushton, C. K., Thomas, N., Hilton, L. K., ... & Morin, R. D. (2021). Non-coding NFKBIZ 3′ UTR mutations promote cell growth and resistance to targeted therapeutics in diffuse large B-cell lymphoma. bioRxiv, 2021-05. https://doi.org/10.1101/2021.05.22.445261
Bonar, C. D., Han, J., Wang, R., Panchapakesan, S. S. S., & Unrau, P. J. (2022). E. coli 6S RNA complexed to RNA polymerase maintains product RNA synthesis at low cellular ATP levels by initiation with noncanonical initiator nucleotides. RNA, 28(12), 1643-1658. https://doi.org/10.1261/rna.079356.122
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01:04:45"},"info":{"id":18431,"cat_no_base":"E041","parent_id":3209,"description":"\u003Cp\u003E\u003Cstrong\u003ET7 RNA Polymerase\u003C\/strong\u003E is a \u003Cstrong\u003EDNA-dependent enzyme\u003C\/strong\u003E that catalyzes the synthesis of RNA in the \u003Cstrong\u003E5\u0027 to 3\u0027 direction\u003C\/strong\u003E. It works exclusively in the presence of its \u003Cstrong\u003ET7 phage promoter sequence\u003C\/strong\u003E, ensuring \u003Cstrong\u003Ehigh specificity\u003C\/strong\u003E for transcription. This enzyme \u003Cstrong\u003Edoes not recognize SP6 or T3 RNA Polymerase promoter sequences\u003C\/strong\u003E, making it ideal for applications requiring \u003Cstrong\u003Eprecise and controlled RNA production\u003C\/strong\u003E from T7-driven templates.\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin: 30px 0;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ET7 RNA Polymerase (50 U\/\u0026mu;l)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l (5000 U)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E10X T7 RNA Polymerase Reaction Buffer\u003C\/td\u003E\n\u003Ctd\u003E1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003ESynthesis of RNA transcripts for hybridization probes\u003C\/li\u003E\n\u003Cli\u003ESynthesis of RNA for in vitro translation\u003C\/li\u003E\n\u003Cli\u003ESynthesis of biologically active mRNA\u003C\/li\u003E\n\u003Cli\u003EGenerate large amounts of labelled or non-labelled RNA\u003C\/li\u003E\n\u003C\/ul\u003E","components":"Enzyme supplied with 10X Reaction Buffer","cas9_origin":null,"concentration":"50 U\/ul","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":"50 mM Tris-HCl (pH 8), 1 mM EDTA, 20 mM \u03b2-ME, 100 mM NaCl, 0.1% Triton\u00aeX-100 and 50% (v\/v) Glycerol.","caution":null,"storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":null,"note":"\u003Cp\u003EOne unit is defined as the amount of T7 RNA Polymerase that is required to incorporate 1 nmol ATP into acid-insoluble material in a 50 \u0026mu;l reaction volume in 1 hour at 37\u0026deg;C in 1X T7 RNA Polymerase Reaction Buffer.\u003C\/p\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2023-02-27 00:33:26","updated_at":"2025-03-13 20:25:32","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379480,"parent_id":3209,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/t\/7\/t7-rna-polymerase-01.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18084837,"sku_in_m2":"E041","value_id_m2":49910082,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"}],"gene":null}