Cas9 Nuclease GFP NLS Protein (High Concentration)
Cat. No.
K148
Unit
47µg (250pmol/ 25µL)
Price
$162.00
| Cat. No. | K148 | |
| Name | Cas9 Nuclease GFP NLS Protein (High Concentration) | |
| Unit | 47µg (250pmol/ 25µL) | |
| Category | Cas Proteins & CRISPR Screening | |
| Description |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
|
|
| Cas Type | Nuclease | |
| Cas Origin | spCas9 | |
| Cas Protein Marker | GFP | |
| Concentration | 10 µM, 1.88 mg/ml | |
| Format General | Enzyme supplied with 10X Reaction Buffer | |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. | |
| Storage Condition | Store all components at -20°C. | |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K148 |
Datasheet
Search CoA here
MSDS
| Does K148 contain a HIS-tag? | |
|
Yes, it does contain a His-tag.
|
- Majeau, N., et al. (2022). Serum extracellular vesicles for delivery of CRISPR-CAS9 ribonucleoproteins to modify the dystrophin gene. Molecular Therapy, 30(7), 2429–2442. https://doi.org/10.1016/j.ymthe.2022.05.023
- Majeau, N., Fortin-Archambault, A., Gérard, C., Rousseau, J., Yaméogo, P., & Tremblay, J. P. (2022). Serum extracellular vesicles for delivery of CRISPR-CAS9 ribonucleoproteins to modify the dystrophin gene. Molecular Therapy, 30(7), 2429–2442. https://doi.org/10.1016/j.ymthe.2022.05.023
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The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).\u003C\/p\u003E\n\u003Cp\u003E\u003Cbr \/\u003EThe Cas9 from the bacteria \u003Cem\u003EStreptococcus pyogenes\u003C\/em\u003E, abbreviated spCas9, is the most commonly used Cas9 variant. The fusion of Cas9 Nuclease NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Nuclease-GFP can also be used for FACS applications and screening. Cas9 Nuclease-GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. 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