BlasTaq™ Probe 2X qPCR MasterMix
Cat. No.
G890
Unit
500 rxn (4 x 1.25 ml)
Price
$139.00
| Cat. No. | G890 | ||||||||
| Name | BlasTaq™ Probe 2X qPCR MasterMix | ||||||||
| Unit | 500 rxn (4 x 1.25 ml) | ||||||||
| Category | qPCR Mastermixes | ||||||||
| Description |
BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan™ probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix — including dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and other proprietary buffer components — have been optimized to deliver superb sensitivity and performance in under one hour. BlasTaq™ is a next-generation, strategically engineered DNA polymerase that offers faster extension rates, improved processivity, and enhanced sensitivity compared to wild-type Taq polymerase. It possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, which is essential for TaqMan probe hydrolysis during qPCR. It does not have 3’-5’ exonuclease (proofreading) activity, and produces 3’-dA-tailed amplicons, which are compatible with TA cloning if needed. This MasterMix is suitable for a variety of applications, including SNP genotyping assays, gene expression analysis, microarray validation, and high-throughput screening. ROX reference dye is provided separately, ensuring universal compatibility with most qPCR instruments. Refer to the Rox Machine Compatibility Chart for instrument-specific guidelines. Product Features:
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
BlasTaq™ Probe 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G890 |
Datasheet
| Can BlasTaq™ Probe 2X qPCR MasterMix (Cat. G890) be used for multiplexing assays? | |
|
Yes, BlasTaq™ Probe 2X qPCR MasterMix (Cat. G890) can be used for multiplexing assays.
|
| Does BlasTaq™ Probe 2X qPCR MasterMix contain an antibody? | |
|
No, BlasTaq™ Probe 2X qPCR MasterMix does not contain an antibody. It includes dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and proprietary buffer components, making it ideal for TaqMan probe-based real-time qPCR analysis and various applications like SNP genotyping and gene expression analysis.
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| How does BlasTaq™ differ from regular Taq polymerase? | |
|
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
|
| Can I use this MasterMix with any qPCR machine? | |
|
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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| What is the recommended amount of template DNA to use in each reaction? | |
|
Typically, 100 ng of genomic DNA is used as template in a 20 μl reaction, but the volume can be adjusted depending on the application.
|
| What is the difference between probe-based and dye-based qPCR assays? | |
|
Probe-based assays, like those using TaqMan probes, rely on a specific probe that binds to the target DNA sequence and emits a fluorescent signal when cleaved during amplification. This provides high specificity for detecting the target sequence. Dye-based assays, like those using SYBR Green or EvaGreen, rely on a dye that binds to double-stranded DNA, emitting fluorescence as the amount of DNA increases during amplification. Dye-based assays are simpler but may be less specific since the dye binds to any double-stranded DNA, not just the target sequence. BlasTaq™ Probe 2X qPCR MasterMix is specifically designed for probe-based assays, offering higher specificity, while dye-based assays use MasterMixes like BlasTaq™ 2X qPCR MasterMix (Cat. No. G891). |
| Can I use BlasTaq™ Probe 2X qPCR MasterMix for high-throughput screening? | |
|
Yes, BlasTaq™ Probe 2X qPCR MasterMix is suitable for high-throughput screening applications, as well as other applications like SNP genotyping and gene expression analysis.
|
- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
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Aydin E, Gunduz MK, Kaymak G, Sezgin AK, Dağgez H, Renders DP, Yakupoğullari Y. Antimycobacterial activity of luteolin in resistant Mycobacterium tuberculosis isolates and cytotoxicity on L929 cells. Microb Pathog. 2025 Mar;200:107287. https://doi.org/10.1016/j.micpath.2025.107287. Epub 2025 Jan 10. PMID: 39800168.
Gündüz, M. K., Kar, F., & Kaymak, G. Papain HepG2 Hücrelerinde Kaspaz-3 ve Kaspaz-9 Genlerini Düzenleyerek Apoptozu İndükler. Osmangazi Tıp Dergisi, 45(1), 94-100. https://doi.org/10.20515/otd.1181399
Koldemir Gündüz, M. BGM, a Newly Synthesised Boron Compound, Induces Apoptosis and Reduces Oxidative Stress by Inhibiting Lipogenesis in 3T3-L1 Adipocytes via PPARγ and CTRP3. Biol Trace Elem Res 200, 4807–4816 (2022). https://doi.org/10.1007/s12011-022-03261-z
Nunes, J. R., O’dwyer, C., Ghorbani, P., Smith, T. K., Chauhan, S., Robert-Gostlin, V., ... & Fullerton, M. D. (2024). Myeloid AMPK signaling restricts fibrosis but is not required for metformin improvements during CDAHFD-induced NASH in mice. Journal of Lipid Research, 65(6), 100564. https://doi.org/10.1016/j.jlr.2024.100564
Wu, J., Chen, Q., Wang, Y., Wang, R., Chen, Q., Wang, Y., ... & Chen, K. (2024). LINC01977 promotes colorectal cancer growth and metastasis by enhancing aerobic glycolysis via the ERK/c-Myc axis. Journal of Gastrointestinal Oncology, 15(1), 271. https://doi.org/10.21037/jgo-24-52
Xia, Y., Cheng, H., Bian, W., Wang, W., Zhu, M., & Zhong, J. (2022). Role of an FNIP repeat domain-containing protein encoded by Megavirus baoshan during viral infection. Journal of Virology, 96(14), e00813-22. https://doi.org/10.1128/jvi.00813-22
Zhao Z, Wang B, Jiang K, Liu M, Wang L. Comparative analysis of transcriptomics and metabolomics provides insights into the mechanisms of VPAHPND invasion and hepatopancreatic damage in Litopenaeus vannamei. Fish Shellfish Immunol. 2024 Nov;154:109920. https://doi.org/10.1016/j.fsi.2024.109920. Epub 2024 Sep 24. PMID: 39321907.
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No.\u003C\/th\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022dye-qPCR\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003EDye-based MasterMix\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG891\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G891\u0022\u003E$135.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix-g892.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix-g892.html\u0022 rel=\u0022noopener\u0022\u003EG892\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E2,500 rxn (25.0 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G892\u0022\u003E$545.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022probe-qPCR\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003EProbe-based MasterMix\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; Probe 2X qPCR MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-2x-qpcr-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG890\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G890\u0022\u003E$135.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022qpcr-kit\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003E\u003Cstrong\u003ERT-qPCR Kit\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-one-step-rt-qpcr.html\u0022 rel=\u0022noopener\u0022\u003EBlasTaq\u0026trade; Probe One-Step RT-qPCR\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-one-step-rt-qpcr.html\u0022 rel=\u0022noopener\u0022\u003EG596\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G596\u0022\u003E$115.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr\u0022 id=\u0022qpcr\u0022 data-text=\u0022qPCR MasterMixes\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cb\u003EqPCR MasterMixes\u003C\/b\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EStreamline your qPCR with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s MasterMixes. Our specially formulated MasterMixes contain our next generation enzymes as well as all the ingredients you need premixed into an optimized format, significantly simplifying your workflow. Enjoy reduced handling errors, high reproducibility and excellent yields.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1.2em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/premixed-and-ready-to-use-icon.png\u0022 \/\u003E\n\u003Cp\u003EPremixed and ready-to-use\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/simple-streamlined-for-high-throughput-use-icon.png\u0022 \/\u003E\n\u003Cp\u003ESimple and streamlined for high-throughput use\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/reduce-handling-errors-icon.png\u0022 \/\u003E\n\u003Cp\u003EReduce handling errors\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/MasterMixes-Kits-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EReliable Detection\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/blastaq-2x-qpcr-mastermix.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlastaq\u0026trade; 2X qPCR MasterMix reliable quantifies low expression genes.\u003C\/strong\u003E Expression levels of 10 target genes in Human Colorectal Cancer Lines were quantified using Blastaq\u0026trade; 2X qPCR MasterMix (Cat. No. G891). Values were normalized to GAPDH. Data courtesy of Dr. Vivek Vaish from Pune University.\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u0026nbsp;\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EOutstanding Sensitivity\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/MasterMixes-Kits-data-11.png\u0022 \/\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EBlasTaq\u0026trade; Probe One-Step RT-qPCR has superb sensitivity and signal-to-noise ratio. \u003C\/strong\u003E 20 \u0026micro;l One-Step RT-qPCR reactions (20 ng, 2 ng, 200 pg, 20 pg, or 2 pg of RNA per reaction) were carried out with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s BlasTaq\u0026trade; Probe One-Step RT-qPCR (Cat. No. G596) for GAPDH amplification.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-page-left-link\u0022 id=\u0022free_sample\u0022 data-text=\u0022Request a Free Sample\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESimply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1600px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E \u003C!-- onload=\u0022window.parent.scrollTo(0,0)\u0022--\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-introduction\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/Resources_DNA-Polymerase-Variations.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to PCR\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"qPCR Mastermixes \u0026 Kits","meta_keywords":null,"meta_description":null,"deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":104,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":894,"cat_no_base":"G890","parent_id":108,"description":"\u003Cp\u003E\u003Cstrong\u003EBlasTaq\u0026trade; Probe 2X qPCR MasterMix\u003C\/strong\u003E is designed for \u003Cstrong\u003ETaqMan\u0026trade; probe-based \u003C\/strong\u003Ereal-time qPCR analysis of DNA samples. The components in the MasterMix \u0026mdash; including dNTPs, \u003Cstrong\u003EBlasTaq\u0026trade; DNA polymerase\u003C\/strong\u003E, MgCl\u2082, and other proprietary buffer components \u0026mdash; have been optimized to deliver \u003Cstrong\u003Esuperb sensitivity and performance in under one hour\u003C\/strong\u003E.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EBlasTaq\u0026trade;\u003C\/strong\u003E is a next-generation, strategically engineered DNA polymerase that offers f\u003Cstrong\u003Easter extension rates, improved processivity, and enhanced sensitivity\u003C\/strong\u003E compared to wild-type Taq polymerase. It possesses 5\u0026rsquo;-3\u0026rsquo; polymerase activity and 5\u0026rsquo;-3\u0026rsquo; exonuclease activity, which is essential for TaqMan probe hydrolysis during qPCR. It does not have 3\u0026rsquo;-5\u0026rsquo; exonuclease (proofreading) activity, and produces 3\u0026rsquo;-dA-tailed amplicons, which are compatible with TA cloning if needed.\u003C\/p\u003E\n\u003Cp\u003EThis MasterMix is suitable for a variety of applications, including \u003Cstrong\u003ESNP genotyping assays, gene expression analysis, microarray validation, and high-throughput screening\u003C\/strong\u003E. \u003Cstrong\u003EROX reference dye\u003C\/strong\u003E is provided separately, ensuring universal compatibility with most qPCR instruments. Refer to the \u003Ca href=\u0022\/uploads\/document\/ROX%20Machine%20Compatibility%20List_v1.pdf\u0022\u003ERox Machine Compatibility Chart\u003C\/a\u003E for instrument-specific guidelines.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EDesigned for TaqMan Probe-based qPCR (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003EDye-based qPCR MasterMix also available\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003E40-70% less time than regular Taq\u003C\/li\u003E\n\u003Cli\u003E6 kb\/min extension speed\u003C\/li\u003E\n\u003Cli\u003EHighly robust and sensitive\u003C\/li\u003E\n\u003Cli\u003EProbe-based One-Step RT-qPCR kit also available (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-probe-one-step-rt-qpcr.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G596\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp style=\u0022margin: 20px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E \u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EBlasTaq\u0026trade;\u0026nbsp;Probe\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E2X qPCR MasterMix\u003C\/td\u003E\n\u003Ctd\u003E500 rxn (4 x 1.25 ml)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EROX Reference Dye\u003C\/td\u003E\n\u003Ctd\u003E50 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022vertical-align: middle;\u0022\u003E\n\u003Ctd style=\u0022text-align: left; vertical-align: middle;\u0022 width=\u002215%\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\u0022 alt=\u0022ISO 13485:2016 MDSAP Certified\u0022 width=\u002285%\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003EISO 13485:2016 MDSAP Certified\u003C\/strong\u003E\u003Cbr \/\u003EOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp align=\u0022left\u0022\u003EStore at -20\u0026deg;C.\u0026nbsp;This product is stable for 2 years from the date of shipping if stored and handled properly.\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":"PCR-1A","note":"\u003Cp\u003EBlasTaq\u0026trade; Probe 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E\n\u003Cdiv\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EMACHINE TYPE\u003C\/th\u003E\n\u003Cth\u003EMACHINE\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EHigh ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026nbsp;\u0026reg; 7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u0026trade;, StepOne\u0026trade;, OpenArray, PRISM\u0026trade; Sequencing Detection Series\u003C\/li\u003E\n\u003Cli\u003EBiometra TOptical\u003C\/li\u003E\n\u003Cli\u003EFluidigm BioMark\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EWafergene SmartChip System\u003C\/li\u003E\n\u003Cli\u003ETianLong TL998 System\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ELow ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026reg; 7500, 7500 Fast, Viia\u0026trade; 7, QuantStudio, QuantStudio 3\/5\/6\/7\u003C\/li\u003E\n\u003Cli\u003EBioGene InSyte\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EIllumina Eco\u003C\/li\u003E\n\u003Cli\u003EAnalytikjena qTower Series\u003C\/li\u003E\n\u003Cli\u003EStratagene\u0026reg; Mx3000, Mx3005, Mx4000\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENo ROX machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EBioRad\u0026reg; CFX96, CFX384, Chromo4\u0026trade;, CFX Connect\u0026trade;, Opticon 2, MiniOpticon\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ERoche LightCycler\u0026reg; (480, 1536, Nano)\u003C\/li\u003E\n\u003Cli\u003EMJ Research Opticon\u0026trade;, Opticon\u0026trade; 2, Chromo\u0026reg; 4\u003C\/li\u003E\n\u003Cli\u003EEppendorf\u0026trade; Realplex 4\u003C\/li\u003E\n\u003Cli\u003EBioGene SynChron\u0026trade;\u003C\/li\u003E\n\u003Cli\u003ECorbett Rotor-gene\u0026reg; (3000, 6200, 62H0, 6500, 65H0, 6600)\u003C\/li\u003E\n\u003Cli\u003EEppendorf Mastercycler\u0026reg; realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)\u003C\/li\u003E\n\u003Cli\u003ECepheid SmartCyler, GeneXpert\u003C\/li\u003E\n\u003Cli\u003EEnigma\u0026trade; ML\u003C\/li\u003E\n\u003Cli\u003EIdaho LightScanner\u0026reg; (24, 32), RapidCycler\u0026reg;2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS\u003C\/li\u003E\n\u003Cli\u003EQiagen Rotor-Gene\u0026trade; (Q, 6000)\u003C\/li\u003E\n\u003Cli\u003ETakara Dice\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EThermo Scientific PikoReal\u003C\/li\u003E\n\u003Cli\u003EDNA-Technology DT96, DTlite, DT-322\u003C\/li\u003E\n\u003Cli\u003EBioer LineGene (3310\/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)\u003C\/li\u003E\n\u003Cli\u003EBioneer Exicycler\u0026trade;\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E","recommend":"\u003Cstyle\u003E\n.orange-button {\nbackground-color: #e96225; \/* orange *\/\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\nwidth: 100%;\nline-height: 1em;\n}\n\n.blue-button {\nbackground-color: #1b7cef;\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\n}\n\n.table-style{\ntext-align: left;\nborder-bottom: 2px solid #f4f4f4;\n}\n\n.left{\nfloat: left;\nwidth: 48%;\n}\n\n.col-3 {\nfloat: left;\nwidth: 33%;\n}\n\n.col-3-right{\nfloat: right;\nwidth: 32%;\n}\n\n.right{\nfloat: right;\nwidth: 48%;\n}\n\n.clear{\nclear: both;\n}\n.spacing{\npadding-right: 2.5em;\n}\n\n.orange-border {\n border-radius: 0.75em;\n border: 4px solid #E96225;\n padding: 0.75em 2em 0.75em;\n}\n\n\n@media only screen and (max-width: 700px) {\n .left{\n width: 100%;\n }\n\n.right{\nwidth: 100%;\n}\n\n\n.col-3 {\nwidth: 100%;\n}\n\n.mobile{\nwidth: 100%;\n}\n\n.spacing{\npadding-right: 0;\n}\n}\n\u003C\/style\u003E\n\u003Cdiv class=\u0022left\u0022\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/pcr-buffet-program\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/pcr-buffet-product-page-banner.png\u0022 alt=\u0022PCR buffet\u0022 width=\u0022100%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022right\u0022\u003E\u003C\/div\u003E","depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":null,"updated_at":"2025-03-25 18:01:51","short_description":"\u003Cp\u003EBlasTaq\u003Cspan\u003E\u2122\u003C\/span\u003E\u00a0Probe 2X qPCR MasterMix\u00a0is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaq\u003Cspan\u003E\u2122\u003C\/span\u003E\u00a0DNA polymerase, MgCl\u003Csub\u003E2\u003C\/sub\u003E, and other proprietary buffer components) have been developed for\u00a0superb and sensitive performance in under one hour. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. 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