qPCR AAV Titer Kit
Cat. No.
G931
Unit
100 rxn
Price
$212.00
| Cat. No. | G931 | ||||||||||||||||||
| Name | qPCR AAV Titer Kit | ||||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||||
| Category | qPCR Virus Titer Kits | ||||||||||||||||||
| Description |
abm’s qPCR AAV Titer Kit is a one-step assay featuring a rapid lysis step followed by qPCR, providing high sensitivity and specificity for accurate AAV titer quantification. Designed to minimize non-specific background, the kit outperforms similar products in both reliability and performance. The MasterMix contains a dye similar to SYBR Green™ and EvaGreen®, enhancing amplification efficiency, while the ROX reference dye is provided separately to ensure compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our ROX Machine Compatibility Chart.
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
qPCR AAV Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G931 |
| What samples were used to create the standards in this kit? | |
|
Plasmid with partial virus DNA were used to generate the standards.
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| Which vector elements are amplified and what is the general location of the primers? | |
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The primers target the 3'ITR region.
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| Is this kit able to detect all AAV serotypes? | |
|
Yes, this kit should be able to detect all AAV serotypes. If in doubt, we can also verify for you if you can send us your ITR sequences.
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| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
|
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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Tanaka, Y., Kadota, S., Zhao, J. et al. Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin. Stem Cell Res Ther 14, 240 (2023). https://doi.org/10.1186/s13287-023-03468-4
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Boss‐Kennedy, A., Kim, D., Barai, P., Maldonado, C., Reyes‐Ordoñez, A., & Chen, J. (2024). Muscle cell‐derived Ccl8 is a negative regulator of skeletal muscle regeneration. The FASEB Journal, 38(14), e23841. https://doi.org/10.1096/fj.202400184R
Deroyer, C., Poulet, C., Paulissen, G. et al. CEMIP (KIAA1199) regulates inflammation, hyperplasia and fibrosis in osteoarthritis synovial membrane. Cell. Mol. Life Sci. 79, 260 (2022). https://doi.org/10.1007/s00018-022-04282-6
Ishiwata, R., & Morimoto, Y. (2022). Hyperphosphatemia-induced degradation of transcription factor EB exacerbates vascular calcification. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1868(3), 166323. https://doi.org/10.1016/j.bbadis.2021.166323
Kirk, R. W., Sun, L., Xiao, R., Clark, E. A., & Nelson, S. (2025). Multiplexed CRISPRi Reveals a Transcriptional Switch Between KLF Activators and Repressors in the Maturing Neocortex. bioRxiv, 2025-02. https://doi.org/10.1101/2025.02.07.636951
Li, Y., Nguyen, J. T., Ammanamanchi, M., Zhou, Z., Harbut, E. F., Mondaza-Hernandez, J. L., ... & Bleris, L. (2023). Reduction of Tumor Growth with RNA-Targeting Treatment of the NAB2–STAT6 Fusion Transcript in Solitary Fibrous Tumor Models. Cancers, 15(12), 3127. https://doi.org/10.3390/cancers15123127
McGinnis, J. P., Ortiz-Guzman, J., Guevara, M. C., Mallannagari, S., Belfort, B. D., Bao, S., ... & Arenkiel, B. R. (2024). Common AAV gene therapy vectors show indiscriminate transduction of living human brain cell types. bioRxiv. https://doi.org/10.1101/2024.11.14.623624
Tanaka, Y., Kadota, S., Zhao, J. et al. Mature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin. Stem Cell Res Ther 14, 240 (2023). https://doi.org/10.1186/s13287-023-03468-4
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No. G931) containing primers for both CMV and ITR. The titer results for each serotype show there is no significant difference in real-time PCR amplification for CMV or ITR.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EEasy Visualization\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/qPCR-lenti-titer-kit-graph.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003Eabm\u0026rsquo;s qPCR Lentivirus Titer Kit enables easy visualization of viral titers.\u003C\/strong\u003E A lentivirus sample (blue) was processed following the protocol for the qPCR Lentivirus Titer Kit (Cat. 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7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u0026trade;, StepOne\u0026trade;, OpenArray, PRISM\u0026trade; Sequencing Detection Series\u003C\/li\u003E\n\u003Cli\u003EBiometra TOptical\u003C\/li\u003E\n\u003Cli\u003EFluidigm BioMark\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EWafergene SmartChip System\u003C\/li\u003E\n\u003Cli\u003ETianLong TL998 System\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ELow ROX Machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EABI\u0026reg; 7500, 7500 Fast, Viia\u0026trade; 7, QuantStudio, QuantStudio 3\/5\/6\/7\u003C\/li\u003E\n\u003Cli\u003EBioGene InSyte\u0026trade;\u003C\/li\u003E\n\u003Cli\u003EIllumina Eco\u003C\/li\u003E\n\u003Cli\u003EAnalytikjena qTower Series\u003C\/li\u003E\n\u003Cli\u003EStratagene\u0026reg; Mx3000, Mx3005, Mx4000\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENo ROX machines\u003C\/td\u003E\n\u003Ctd\u003E\n\u003Cul\u003E\n\u003Cli\u003EBioRad\u0026reg; 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