qPCR Retrovirus Titer Kit
Cat. No.
G949
Unit
100 rxn
Price
$212.00
| Cat. No. | G949 | ||||||||||||||||
| Name | qPCR Retrovirus Titer Kit | ||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||
| Category | qPCR Virus Titer Kits | ||||||||||||||||
| Description |
abm’s qPCR Retrovirus Titer Kit is a one-step assay that employs a quick RNA extraction step that is followed by RT-qPCR, providing high sensitivity and specificity for accurate retrovirus titer quantification. Designed to minimize non-specific background, the kit offers superior performance compared to other products on the market. The MasterMix contains a dye similar to SYBR Green™ and EvaGreen™, enhancing amplification efficiency, while the ROX Reference Dye is provided separately for broad compatibility with most qPCR instruments. For detailed instrument compatibility, refer to our ROX Machine Compatibility Chart.
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Note |
qPCR Retrovirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G949 |
| What is the primer design based on? | |
|
The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
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| How come my cells are not successfully infected with the evaluated titer from the kit? | |
|
One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
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| Does this have ROX reference dye? | |
|
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
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| How much ROX reference dye should I add to the MasterMix? | |
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The recommended volume of ROX reference dye varies based on the qPCR instrument:
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| Can I use this MasterMix with any qPCR machine? | |
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Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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- Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
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Colson C, Wang Y, Atherton J, Su X. SLC45A4 encodes a mitochondrial putrescine transporter that promotes GABA de novo synthesis. bioRxiv [Preprint]. 2024 Jul 24:2024.07.23.604788. https://doi.org/10.1101/2024.07.23.604788. PMID: 39091866; PMCID: PMC11291067.
Zhang, Y., Bassal, M., Friedrich, D., Ummarino, S., Verbiest, T., Zhang, J., ... & Tenen, D. G. (2021). C/EBPα acts as an RNA binding protein essential for its chromatin recruitment and promotes Macrophage differentiation. bioRxiv, 2021-10. https://doi.org/10.1101/2021.10.20.465166
This product has no review yet.
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No. G931) containing primers for both CMV and ITR. The titer results for each serotype show there is no significant difference in real-time PCR amplification for CMV or ITR.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EEasy Visualization\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/qPCR-lenti-titer-kit-graph.png\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\u003Cbr \/\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003Eabm\u0026rsquo;s qPCR Lentivirus Titer Kit enables easy visualization of viral titers.\u003C\/strong\u003E A lentivirus sample (blue) was processed following the protocol for the qPCR Lentivirus Titer Kit (Cat. 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Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1600px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E \u003C!-- onload=\u0022window.parent.scrollTo(0,0)\u0022--\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResources\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-introduction\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/qpcr\/Resources_DNA-Polymerase-Variations.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to PCR\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"qPCR Virus Titer Kits","meta_keywords":"qPCR Virus Titer Kits","meta_description":"qPCR Virus Titer Kits","deleted_at":null,"enable":"Y","parent_list":"8","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":745,"cat_no_base":"G949","parent_id":81,"description":"\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s \u003Cstrong\u003EqPCR Retrovirus Titer Kit \u003C\/strong\u003Eis a one-step assay that \u003Cstrong\u003Eemploys a quick RNA extraction step that is followed by RT-qPCR\u003C\/strong\u003E, providing \u003Cstrong\u003Ehigh sensitivity and specificity\u003C\/strong\u003E for accurate retrovirus titer quantification. 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