Poly(A) Polymerase, Yeast
Cat. No.
E017
Unit
100 U (100 μl)
Price
$125.00
| Cat. No. | E017 | ||||||||||
| Name | Poly(A) Polymerase, Yeast | ||||||||||
| Unit | 100 U (100 μl) | ||||||||||
| Category | Molecular Biology Enzymes and Kits | ||||||||||
| Description |
Poly(A) Polymerase, Yeast catalyzes the template-independent addition of adenosine residues to the 3’ ends of RNA molecules using ATP as a substrate to create a poly(A) tail. When cordycepin-5'-triphosphate (3’-dATP) is substituted for ATP, the enzyme adds a single dA residue to the 3’-terminus of RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast is particularly effective for oligonucleotide-labeling and poly(A) tailing of long RNA templates, making it a superior choice compared to E. coli-derived enzymes. This enzyme is ideal for applications that require precise polyadenylation, such as RNA sequencing and cDNA synthesis.
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| Application |
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| Concentration | 1 U/μl | ||||||||||
| Components | Enzyme supplied with 5X Reaction Buffer. | ||||||||||
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol. | ||||||||||
| Storage Condition | Store all components at -20°C. | ||||||||||
| Note |
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. |
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| Caution | This product is distributed for laboratory research only. Not for diagnostic use . | ||||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E017 |
Datasheet
| What are the key differences between Yeast and E. coli Poly(A) Polymerase? | |||||||||||||||||||
|
| Can I use dATP or ADP as a substitute for ATP in the reaction? | |
|
No, Poly(A) Polymerase from yeast specifically requires ATP for polyadenylation. While cordycepin-5’-triphosphate (3’-dATP) can be used for 3’-end labeling, neither ADP nor dATP function as substrates.
|
| How do I stop the reaction after poly(A) tailing? | |
|
The reaction can be terminated by heating at 65°C for 20 minutes or by adding 5 mM EDTA.
|
| Can I use labeled ATP for poly(A) tailing? | |
|
Yes, radiolabeled, biotinylated, or fluorescently labeled ATP can be substituted in the reaction for specific experimental applications.
|
| What are the storage conditions for this enzyme? | |
|
Store all components at -20°C and avoid repeated freeze-thaw cycles to maintain maximum performance. The components are stable for one year from the date of shipping when stored properly.
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- Yoon, Y. M., Go, G., Yoon, S., Lim, J. H., Lee, G., Lee, J. H., & Lee, S. H. (2021). Melatonin Treatment Improves Renal Fibrosis via miR-4516/SIAH3/PINK1 Axis. Cells, 10(7), 1682. https://doi.org/10.3390/cells10071682
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Alghamdi, T. A., Batchu, S. N., Hadden, M. J., Yerra, V. G., Liu, Y., Bowskill, B. B., ... & Advani, A. (2018). Histone H3 serine 10 phosphorylation facilitates endothelial activation in diabetic kidney disease. Diabetes, 67(12), 2668-2681. https://doi.org/10.2337/db18-0124
Özkan, H., Keçeli, H. H., Kaya, U., Dalkiran, S., Yüksel, M., Tek, E., & Yakan, A. (2024). Considering potential roles of selected MicroRNAs in evaluating subclinical mastitis and Milk quality in California mastitis test (+) and infected bovine milk. Animal Science Journal, 95(1), e13959. https://doi.org/10.1111/asj.13959
Sönmez, G., & İnal, Ş. (2022). microRNA expression patterns associated with average daily weight gain in Kangal. https://hdl.handle.net/20.500.12395/45671
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rel=\u0022noopener\u0022\u003EProteinase K\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; width: 14.6197%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/proteinase-k-g029.html\u0022 rel=\u0022noopener\u0022\u003EG029\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; width: 27.1058%;\u0022\u003E1.0 ml; 20mg\/ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G029\u0022\u003E$92.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Molecular Biology Enzymes and Kits","meta_keywords":"Molecular Biology Enzymes and Kits","meta_description":"Molecular Biology Enzymes and Kits","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":18430,"cat_no_base":"E017","parent_id":3208,"description":"\u003Cp\u003E\u003Cstrong\u003EPoly(A) Polymerase, Yeast\u003C\/strong\u003E catalyzes the template-independent addition of adenosine residues to the 3\u0026rsquo; ends of RNA molecules using ATP as a substrate to create a poly(A) tail. When cordycepin-5\u0027-triphosphate (3\u0026rsquo;-dATP) is substituted for ATP, the enzyme adds a single dA residue to the 3\u0026rsquo;-terminus of RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast is particularly effective for \u003Cstrong\u003Eoligonucleotide-labeling and poly(A) tailing of long RNA templates\u003C\/strong\u003E, making it a superior choice compared to E. coli-derived enzymes. This enzyme is ideal for applications that require \u003Cstrong\u003Eprecise polyadenylation,\u003C\/strong\u003E such as \u003Cstrong\u003ERNA sequencing\u003C\/strong\u003E and \u003Cstrong\u003EcDNA synthesis\u003C\/strong\u003E.\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin: 20px 0;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EPoly(A) Polymerase, Yeast (1 U\/\u0026micro;l)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E5X Poly(A) Polymerase, Yeast Reaction Buffer\u003C\/td\u003E\n\u003Ctd\u003E1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E25 mM MnCl\u2082\u003C\/td\u003E\n\u003Ctd\u003E500 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EATP (10 mM)\u003C\/td\u003E\n\u003Ctd\u003E150 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003ELabelling of RNA with ATP or cordycepin\u003C\/li\u003E\n\u003Cli\u003EPoly(A) tailing of RNA for cloning or affinity purification\u003C\/li\u003E\n\u003Cli\u003EIncreasing translation of RNA transferred into eukaryotic cells\u003C\/li\u003E\n\u003C\/ul\u003E","components":"Enzyme supplied with 5X Reaction Buffer.","cas9_origin":null,"concentration":"1 U\/\u03bcl","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":"20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton\u00ae X-100 and 50% (v\/v) Glycerol.","caution":"This product is distributed for laboratory research only. Not for diagnostic use .","storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":null,"note":"\u003Cp\u003EOne unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37\u0026deg;C.\u003C\/p\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2023-02-27 00:32:49","updated_at":"2025-03-07 22:52:00","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379469,"parent_id":3208,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/p\/o\/poly-a--polymerase-yeast_v2.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18084826,"sku_in_m2":"E017","value_id_m2":49910070,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"}],"gene":null}