Poly(A) Polymerase, E. coli
Cat. No.
E099
Unit
25 U (25 µl)
Price
$129.00
| Cat. No. | E099 | ||||||||
| Name | Poly(A) Polymerase, E. coli | ||||||||
| Unit | 25 U (25 µl) | ||||||||
| Category | Molecular Biology Enzymes and Kits | ||||||||
| Description |
Poly(A) Polymerase, E. coli (E-PAP) catalyzes the template-independent addition of adenosine residues to the 3’ ends of RNA, efficiently creating a poly(A) tail. Unlike yeast-derived enzymes, E-PAP works in a sequence-independent manner, meaning it does not require specific recognition sequences to function. This allows for rapid polyadenylation at virtually any unprotected 3’ RNA terminus, regardless of secondary structure. E-PAP is ideal for applications that require poly(A) tail addition to a wide range of RNA molecules, including RNA sequencing and cDNA synthesis. Its versatility and efficiency make it a valuable tool for researchers working with RNA in various experimental setups.
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| Application |
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| Concentration | 1U/µl | ||||||||
| Components | Enzyme is supplied with a 10X Reaction Buffer | ||||||||
| Storage Buffer | 20mM Tris-HCl, 100mM NaCl, 0.1mM DTT, 0.1mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5 @25°C | ||||||||
| Storage Condition | Store all components at -20°C. | ||||||||
| Note |
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C. |
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| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | ||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E099 |
Datasheet
| How long is the typical poly(A) tail generated using this enzyme? | |
|
The Poly(A) Polymerase, E. coli can generate a poly(A) tail of over 100 bases when 1 unit of enzyme is incubated with 1–10 µg of RNA in a 20 µl reaction at 37°C for 30 minutes.
|
| Does this enzyme require a specific sequence for polyadenylation? | |
|
No, unlike Poly(A) polymerase, Yeast, this E. coli enzyme does not require specific recognition sequences and can rapidly add poly(A) tails to nearly all unprotected 3’ RNA termini.
|
| How can I stop the reaction after incubation? | |
|
The reaction should be stopped by immediately proceeding to an RNA clean-up step. abm recommends using their RNA Purification Magnetic Beads (Cat. No. G971) for best results.
|
| What factors influence the length of the poly(A) tail? | |
|
Several factors can affect the tail length, including RNA 3’ hydroxyl end concentration, incubation time, ATP concentration, and the amount of enzyme used.
|
| Can this enzyme be used with structured RNA molecules? | |
|
Yes, this enzyme is effective regardless of RNA secondary structure, as it does not require specific sequences for activity.
|
| What are the key differences between Yeast and E. coli Poly(A) Polymerase? | |||||||||||||||||||
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01:04:45"},"info":{"id":18564,"cat_no_base":null,"parent_id":3386,"description":"\u003Cp\u003E\u003Cstrong\u003EPoly(A) Polymerase, E. coli (E-PAP)\u003C\/strong\u003E catalyzes the template-independent addition of adenosine residues to the 3\u0026rsquo; ends of RNA, efficiently creating a poly(A) tail. Unlike yeast-derived enzymes, E-PAP works in a \u003Cstrong\u003Esequence-independent \u003C\/strong\u003Emanner, meaning it does not require specific recognition sequences to function. This allows for rapid polyadenylation at virtually any unprotected 3\u0026rsquo; RNA terminus, regardless of secondary structure. E-PAP is ideal for applications that require poly(A) tail addition to a wide range of RNA molecules, including RNA sequencing and cDNA synthesis. Its versatility and efficiency make it a valuable tool for researchers working with RNA in various experimental setups.\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin: 20px 0;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EPoly(A) Polymerase, E. coli\u003C\/td\u003E\n\u003Ctd\u003E25 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E10X Poly(A) Polymerase, E. coli Reaction Buffer\u003C\/td\u003E\n\u003Ctd\u003E1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EATP (10 mM)\u003C\/td\u003E\n\u003Ctd\u003E50 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003ELabeling of RNA with ATP or Cordycepin\u003C\/li\u003E\n\u003Cli\u003EPoly(A) tailing of RNA for cloning or affinity purification\u003C\/li\u003E\n\u003Cli\u003EIncreasing translation of RNA transfected into eukaryotic cells\u003C\/li\u003E\n\u003Cli\u003EEnhanced circRNA enrichment by increased degradation of linear \u0026amp; structured RNAs by RNase R\u0026nbsp;\u003C\/li\u003E\n\u003C\/ul\u003E","components":"Enzyme is supplied with a 10X Reaction Buffer","cas9_origin":null,"concentration":"1U\/\u00b5l","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":"20mM Tris-HCl, 100mM NaCl, 0.1mM DTT, 0.1mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5 @25\u00b0C","caution":"This product is distributed for laboratory research only. Not for diagnostic use.","storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":null,"note":"\u003Cp\u003EOne unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 \u0026micro;l volume in 10 minutes at 37\u0026deg;C.\u003C\/p\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2024-06-28 18:44:21","updated_at":"2025-03-07 22:42:58","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":384938,"parent_id":3386,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/qDEm1fIhepE9Lu5PixNARNUHYl0YFLdxK4GknqEN.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-06-28 18:45:11","updated_at":"2024-06-28 18:45:24"},{"id":384939,"parent_id":3386,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/E2MpJ8wnozZYIqLYnSDPXtOr4ZX8rvJbzQl7KxI2.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-06-28 18:45:18","updated_at":"2024-06-28 18:45:24"}],"gene":null}