OneScript® Hot 5X RT MasterMix
Cat. No.
G590
Unit
100 rxn (400 µl)
Price
$209.00
$189.00
| Cat. No. | G590 | ||||||||
| Name | OneScript® Hot 5X RT MasterMix | ||||||||
| Unit | 100 rxn (400 µl) | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
OneScript® Hot 5X RT MasterMix is a convenient, ready-to-use formulation designed for efficient first-strand cDNA synthesis. This product simplifies the cDNA synthesis process, ensuring reliability and reproducibility. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a carefully balanced ratio of Oligo(dT)s and Random Primers. The resulting high-quality cDNA is suitable for a wide range of downstream applications. Product Features:
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| Application |
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| Concentration | 5X | ||||||||
| Storage Condition |
Store at -20°C. |
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| Note |
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592). |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G590 |
Datasheet
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
|
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
|
| Can I synthesize cDNA from long RNA transcripts? | |
|
Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
|
| What can I use the synthesized cDNA for? | |
|
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
|
| How should I store the synthesized cDNA? | |
|
Store the synthesized first-strand cDNA at -20°C for long-term use.
|
| Why am I getting low cDNA yields? | |
|
Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
|
| How much RNA template should I use for cDNA synthesis? | |
|
We recommend using 1 ng to 2 μg of RNA per reaction.
|
| What volumes of cDNA should I use for downstream PCR? | |
|
Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
|
|
What if input RNA samples are expected to have high levels of genomic DNA contamination?
|
|
|
If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
|
| What is the advantage of OneScript® Hot Reverse Transcriptase compared to OneScript® Plus Reverse Transcriptase? | |
|
OneScript® Hot Reverse Transcriptase offers a significant advantage over enzymes that operate at lower temperatures (42-55ºC) due to its exceptional thermostability, allowing it to function effectively at 60-72ºC. This higher temperature improves efficiency by preventing RNA secondary structures and enabling cDNA synthesis from degraded or low-abundance RNA. Additionally, it is more resistant to inhibitors and contaminants, making it ideal for complex or impure RNA samples.
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Do I need an RNase inhibitor when using this product?
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|
|
All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
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| What discontinued abm products is Cat. No. G590 equivalent to? | |
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This product is abm’s next generation of RT MasterMixes and is functionally equivalent to G451, G452, G453, G454 (OneScript® cDNA Synthesis SuperMixes), with improved performance. Contact our customer service team technical@abmgood.com for more information.
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This optimized solution includes \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s proprietary OneScript\u0026reg; Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a perfectly balanced mix of Oligo (dT)s and Random Primers, all in ONE convenient tube!\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003EFor applications using pure RNA where genomic DNA removal is not required, try our \u003Cstrong\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/strong\u003E. It offers a faster protocol for an even more efficient cDNA synthesis.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/genomoic-dna-removal.png\u0022 \/\u003E\n\u003Cp\u003EGenomic DNA Removal*\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/efficient-cdna-synthesis.png\u0022 \/\u003E\n\u003Cp\u003EEfficient cDNA Synthesis\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/easy-one-step-setup.png\u0022 \/\u003E\n\u003Cp\u003EEasy One-Step Setup\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal*\u003C\/a\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Simple\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/strong\u003E is a convenient and ready-to-use formulation for first-strand cDNA synthesis with an easy one-step setup that reduces pipetting and sample handling. Simply add your RNA sample with our mastermix and incubate.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-workflow.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EAccurate cDNA Synthesis\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EGenomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-figure.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Hot RT\u0022 id=\u0022OneScriptHot\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ESynthesize cDNA from complex RNA templates with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase. Engineered for outstanding thermostability, our OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/extreme-thermostability-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EExtreme thermostability at 50-80\u0026deg;C\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/transcribes-degraded-contaminated-samples-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003ETranscribes degraded samples and resists inhibitors \/ contaminants\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/high-cDNA-yields-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EHigh cDNA yields from difficult or ultra-low RNA samples\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-onescript-hot-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022--first-child-width: 50%;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EOneScript\u0026reg; Hot cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EG594\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G594\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EOneScript\u0026reg; Hot Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EG593\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G593\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EMegaFi\u0026trade; One-Step RT-PCR\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EG597\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G597\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EOutstanding Thermostability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-hot-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase has exceptional thermostability. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase (Cat. No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80\u02daC. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Plus RT\u0022 id=\u0022OneScriptPlus\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ERoutine cDNA synthesis can be completed in just 10-15 minutes with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase! 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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-reverse-transcriptase.html\u0022\u003EOneScript\u0026reg; Plus Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-mmlv-reverse-transcriptase.html\u0022\u003EG237\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G237\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EOneScript\u0026reg; Plus cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EG236\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G236\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent Elongation Ability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-plus-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (Cat. No. G237) was used in a reaction with a range of human RNA fragments. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUnsure which reverse transcriptase is right for you?\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which reverse transcriptase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple RT Enzyme Selection Guide below to compare the characteristics of each reverse transcriptase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth width=\u002240%\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptHot\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptPlus\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus\u003C\/a\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EThermostability\u003C\/td\u003E\n\u003Ctd\u003E50-80\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50-55\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOptimal Temperature\u003C\/td\u003E\n\u003Ctd\u003E60\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMaximum cDNA Elongation\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMinimum Sample Input\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EReaction Time\u003C\/td\u003E\n\u003Ctd\u003E10 min\u003C\/td\u003E\n\u003Ctd\u003E15 min\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EInhibitor\/Contamination Resistance\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETolerance to Complex Templates\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESee for yourself why your fellow RT-PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1400px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-variations\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-Variations-to-the-system_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EVariations to the System\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/div\u003E","meta_title":"Reverse Transcription \u0026 RT-PCR","meta_keywords":"Reverse Transcription \u0026 RT-PCR","meta_description":"Reverse Transcription \u0026 RT-PCR","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":103,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":18592,"cat_no_base":null,"parent_id":3412,"description":"\u003Cp\u003E\u003Cstrong\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/strong\u003E is a convenient, ready-to-use formulation designed for efficient first-strand cDNA synthesis. This product simplifies the cDNA synthesis process, ensuring reliability and reproducibility. The optimized 5X RT MasterMix contains \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s proprietary OneScript\u0026reg; Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, dNTPs, and a carefully balanced ratio of Oligo(dT)s and Random Primers. The resulting high-quality cDNA is suitable for a wide range of downstream applications.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EEasy one-step setup reduces pipetting and sample handling\u003C\/li\u003E\n\u003Cli\u003EHigh cDNA yields from difficult samples with OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G593\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003EInhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor-g591.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G591\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003EAlso includes dNTPs, Oligo (dT)s, and random primers\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp style=\u0022margin: 30px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/td\u003E\n\u003Ctd\u003E100 rxn (400 \u0026micro;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENuclease-Free H\u003Csub\u003E2\u003C\/sub\u003EO\u003C\/td\u003E\n\u003Ctd\u003E1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022vertical-align: middle;\u0022\u003E\n\u003Ctd style=\u0022text-align: left; vertical-align: middle;\u0022 width=\u002215%\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\u0022 alt=\u0022ISO 13485:2016 MDSAP Certified\u0022 width=\u002285%\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003EISO 13485:2016 MDSAP Certified\u003C\/strong\u003E\u003Cbr \/\u003EOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003EGeneration of templates for use in RT-PCR and qRT-PCR\u003C\/li\u003E\n\u003Cli\u003EcDNA synthesis from ssRNA\u003C\/li\u003E\n\u003Cli\u003EcDNA library construction\u003C\/li\u003E\n\u003Cli\u003EGeneration of probes for hybridization\u003C\/li\u003E\n\u003Cli\u003EDNA primer extension\u003C\/li\u003E\n\u003C\/ul\u003E","components":null,"cas9_origin":null,"concentration":"5X","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp\u003EStore at -20\u0026deg;C.\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":"PCR-2A","note":"\u003Cp\u003EIf input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s \u003Cstrong\u003EAll-In-One 5X RT MasterMix with gDNA Removal \u003C\/strong\u003E(\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003ECat. No. G592\u003C\/a\u003E).\u003C\/p\u003E","recommend":"\u003Cstyle\u003E\n.orange-button {\nbackground-color: #e96225; \/* orange *\/\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\nwidth: 100%;\nline-height: 1em;\n}\n\n.blue-button {\nbackground-color: #1b7cef;\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\n}\n\n.table-style{\ntext-align: left;\nborder-bottom: 2px solid #f4f4f4;\n}\n\n.left{\nfloat: left;\nwidth: 48%;\n}\n\n.col-3 {\nfloat: left;\nwidth: 33%;\n}\n\n.col-3-right{\nfloat: right;\nwidth: 32%;\n}\n\n.right{\nfloat: right;\nwidth: 48%;\n}\n\n.clear{\nclear: both;\n}\n.spacing{\npadding-right: 2.5em;\n}\n\n.orange-border {\n border-radius: 0.75em;\n border: 4px solid #E96225;\n padding: 0.75em 2em 0.75em;\n}\n\n\n@media only screen and (max-width: 700px) {\n .left{\n width: 100%;\n }\n\n.right{\nwidth: 100%;\n}\n\n\n.col-3 {\nwidth: 100%;\n}\n\n.mobile{\nwidth: 100%;\n}\n\n.spacing{\npadding-right: 0;\n}\n}\n\u003C\/style\u003E\n\u003Cdiv class=\u0022left\u0022\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/pcr-buffet-program\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/pcr-buffet-product-page-banner.png\u0022 alt=\u0022PCR buffet\u0022 width=\u0022100%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022right\u0022\u003E\u003C\/div\u003E","depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2025-01-07 18:03:59","updated_at":"2025-03-27 20:38:11","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":385045,"parent_id":3412,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/LtVcGBsGFRFWwDZ5qoigBfVIC1hoSWwKYKGSQILF.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-01-09 23:14:41","updated_at":"2025-01-09 23:14:41"},{"id":388509,"parent_id":3412,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/6PrfxD29wy2WHL2jqqP8yvNlSqbScHmJE3YIcHY9.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-04 22:58:32","updated_at":"2025-03-04 22:58:32"}],"gene":null}