Pro Ligation-Free Cloning Kit (50 reactions)
Cat. No.
E087
Unit
50 Reactions
Price
$258.00
| Cat. No. | E087 |
| Name | Pro Ligation-Free Cloning Kit (50 reactions) |
| Unit | 50 Reactions |
| Category | Molecular Biology Enzymes and Kits |
| Description |
The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs. Key Kit Features:
Testimonial: "I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit." - Jianfeng Lan, The Buck Institute |
| Application |
|
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E087 |
| Do I need to purify the PCR product first before use with this kit? Or can I use low melting agarose for the PCR gel and just melt the band portion and use in the kit? | |
|
The kit can be used in either scenario.
1. The kit comes with Cloning Optimizer and Assembly Enhancer enzymes which allow the user to not have to do gel extraction/purification following the amplification of DNA fragments, as methylated template DNA and single stranded DNA (e.g. primers) will be removed. 2. If PCR reactions contain multiple non-specific bands, it is highly advisable to either optimize the PCR or perform gel extraction, as the contaminating bands will be present in the cloning reaction if not prior removed. |
- Huang, R., Ding, R., Liu, Y., Li, F., Zhang, Z., & Wang, S. A. 2022. GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous. Microbial Biotechnology, 15(10), 2578-2593.
- Hwang, B.-J., Gonzales, R., Corzine, S., Stenson, E., Pidugu, L., & Lu, A.-L. (2022). DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. PLOS ONE, 17(8), e0272645. https://doi.org/10.1371/journal.pone.0272645
- Li, L., Liu, W., Fan, N., Li, F., Huang, B., Liu, Q., ... & Wang, X. (2022). Scallop IKK1 Responds to Bacterial and Virus-Related Pathogen Stimulation and Interacts With MyD88 Adaptor of Toll-Like Receptor Pathway Signaling. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.869845
- Huang, B., Wu, Y., Ma, J., Yang, B., Sang, X., Chen, J., Liu, W., Li, F., Li, L., Wang, X., Dong, J., & Wang, X. (2022). The first identified invertebrate LGP2-like homolog gene in the Pacific oyster Crassostrea gigas. Fish & Shellfish Immunology, 128, 238–245. https://doi.org/10.1016/j.fsi.2022.08.005
- Sang, X., Liu, W., Li, F., Huang, B., Li, L., Wang, X., Dong, J., Ma, J., Chen, J., & Wang, X. (2022). Scallop RIG-I-like receptor 1 responses to polyinosinic:polycytidylic acid challenge and its interactions with the mitochondrial antiviral signaling protein. Fish & Shellfish Immunology, 124, 490–496. https://doi.org/10.1016/j.fsi.2022.04.042
- Tettey, C. K., Mu, X.-Q., Ma, H.-Y., Chen, X.-Y., Geng, C., Tian, Y.-P., Yan, Z.-Y., & Li, X.-D. (2023). The role of different innate and environmental factors in Tm-22-mediated resistance to tomato mottle mosaic virus. Phytopathology Research, 5(1). https://doi.org/10.1186/s42483-023-00162-4
- Novak, N., Baumann, M., Friss, A., Cairns, V., DeMaria, C., & Borth, N. (2023). LncRNA analysis of mAb producing CHO clones reveals marker and engineering potential. Metabolic Engineering, 78, 26–40. https://doi.org/10.1016/j.ymben.2023.05.003
- Chen, J., Qu, Y., Dong, J., Xu, W., Zhao, Y., Cui, J., Yu, Z., Bao, Z., Ma, J., Han, Y., Liu, Y., Huang, B., & Wang, X. (2024). A scallop IκB protein involved in innate immunity acts as a key regulator of NF-κB. Fish & Shellfish Immunology, 154, 109897. https://doi.org/10.1016/j.fsi.2024.109897
- Huang, B., Ma, J., Xu, W., Cui, J., Chen, J., Qu, Y., Zhao, Y., Han, Y., Liu, Y., Wang, W., & Wang, X. (2024). A newly identified scallop MyD88 interacts with TLR and functions in innate immunity. Fish & Shellfish Immunology, 151, 109697. https://doi.org/10.1016/j.fsi.2024.109697
- Tao, Y., Wang, J., Xiao, L., Zhang, Q., & Guo, H. (2024). Development and Evaluation of a Shrimp Virus (IHHNV)-Mediated System Used for Efficient Gene Transfer and Expression in Shrimps. https://doi.org/10.20944/preprints202406.2070.v1
This product has no review yet.
Controls and Related Product:
{"id":3195,"cat_no":"E087","sku_in_m2":"","old_cat_no":"","name":"Pro Ligation-Free Cloning Kit (50 reactions)","category_id":186,"category_name":"Molecular Biology Enzymes and Kits","frontend_category_id":6,"frontend_category_ids":"86,1,6","gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"50 Reactions","accession_number":"","species_id":0,"species":"","raised_in_id":0,"raised_in":"","cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","price":"258.0000","price_before_discount":"0.0000","keywords":"","invisible_search_keywords":"Seamless assembly, Gibson assembly, Isothermal assembly, Cloning mastermix, Seamless mastermix, Seamless cloning mastermix, ligation free mastermix, Proligation free, Pro ligation free, Cloning mix, Cloning kit, pcr cloning kit, Molecular Biology Kits","filter_group_id":34,"filter_group_name":"Molecular Biology","filter_id":41,"filter_name":"Kits \u0026 Other Enzymes","search_filter_ids":"32,34","search_filter_keys":"32-34","temp_field":null,"customization":0,"filter_table_id":0,"filter_table_name":"","related_group_id":17,"url":"","operator":"","points_rule":7,"status":1,"need_remove":0,"document_sort":"","price_old":"215.0000","price2024":"225.0000","created_at":"2023-01-19 04:37:34","updated_at":"2025-01-06 08:29:53","Confirmed":"Need Confirm","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"Molecular-Biology-Enzymes-and-Kits.html","frontendBreadcrumbs":[{"id":1,"pid":86,"name":"General Materials","page_id":27,"page_type":"App\\Models\\Category","href":null,"sort":1,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"general-materials.html","url":"general-materials.html"},{"id":6,"pid":1,"name":"Enzymes \u0026 Kits","page_id":186,"page_type":"App\\Models\\Category","href":null,"sort":6,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"Molecular-Biology-Enzymes-and-Kits.html","url":"Molecular-Biology-Enzymes-and-Kits.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseMolecular","geo_price":"258.00","geo_price_symbol":"$258.00","category":{"id":186,"categories":"Molecular Biology Enzymes and Kits","pid":27,"url_key":"Molecular-Biology-Enzymes-and-Kits.html","css":"\/assets\/css\/abm.search.css,\/assets\/css\/abm.other.css,\/assets\/css\/abm.categorys.css, \/assets\/css\/ryan.temp.css","wid":0,"breadcrumbs":"[{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\u0022,\u0022title\u0022:\u0022Home\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/general-materials.html\u0022,\u0022title\u0022:\u0022General Materials \u0026amp; Others\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/Molecular-Biology-Enzymes-and-Kits.html\u0022,\u0022title\u0022:\u0022Molecular Biology Enzymes and Kits\u0022}]","breadcrumbsIdJson":"\u003Cscript type=\u0022application\/ld+json\u0022\u003E{\u0022@context\u0022:\u0022https:\/\/schema.org\u0022,\u0022@type\u0022:\u0022BreadcrumbList\u0022,\u0022itemListElement\u0022:[{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:1,\u0022name\u0022:\u0022Home\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:2,\u0022name\u0022:\u0022General Materials \u0026amp; Others\u0022,\u0022item\u0022:\u0022https:\/\/www.abmgood.com\/general-materials.html\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:3,\u0022name\u0022:\u0022Molecular Biology Enzymes and Kits\u0022}]}\u003C\/script\u003E","breadcrumbsHtml":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/\/www.abmgood.com\/general-materials.html\u0022\u003EGeneral Materials \u0026amp; Others\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022active\u0022\u003EMolecular Biology Enzymes and Kits\u003C\/li\u003E\u003C\/ul\u003E","description":"\u003Ch2 class=\u0022abm-categories-title-h2\u0022\u003EMolecular Biology Enzymes and Kits\u003C\/h2\u003E\n\u003Cul class=\u0022abm-page-category-nav-list\u0022 style=\u0022margin-top: -20px;\u0022\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#Cloning-Applications\u0022 class=\u0022abm-page-category-nav-link abm-page-category-target-link\u0022\u003E Cloning Applications\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#DNA-RNA-Applications\u0022 class=\u0022abm-page-category-nav-link abm-page-category-target-link\u0022\u003E DNA \u0026amp; RNA Applications\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#in-vitro-Kits-Proteases\u0022 class=\u0022abm-page-category-nav-link abm-page-category-target-link\u0022\u003EProteases\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022\/rnase-r-e049.html\u0022 class=\u0022abm-page-category-nav-link abm-page-category-target-link\u0022\u003E RNase R\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022\/cas9-proteins.html\u0022 class=\u0022abm-page-category-nav-link abm-page-category-target-link\u0022\u003ECas Proteins \u0026amp; CRISPR Screening\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-md-12\u0022 style=\u0022text-align: left; padding-bottom: 3em;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a variety of high quality individual enzymes and kits that enable the preparation of DNA and RNA for subsequent downstream applications such as assay development, PCR, restriction digest, cloning and sequencing. Simplify your experiments with our trusted products for consistent, high-performance results.\u003C\/p\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022--first-child-white-space: nowrap;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left\u0022 class=\u0022abm-page-menu only-hide\u0022 id=\u0022Cloning-Applications\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000;\u0022\u003E\u003Cstrong\u003ECloning Applications\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/pro-ligation-free-cloning-kit-e086.html\u0022 rel=\u0022noopener\u0022\u003EPro Ligation-Free Cloning Kit (20 reactions)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/pro-ligation-free-cloning-kit-e086.html\u0022 rel=\u0022noopener\u0022\u003EE086\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E20 Reactions\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E086\u0022 data-type=\u0022molecular\u0022\u003E$150.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/pro-ligation-free-cloning-kit-e087.html\u0022 rel=\u0022noopener\u0022\u003EPro Ligation-Free Cloning Kit (50 reactions)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/pro-ligation-free-cloning-kit-e087.html\u0022 rel=\u0022noopener\u0022\u003EE087\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E50 Reactions\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E087\u0022 data-type=\u0022molecular\u0022\u003E$250.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/proclone-competent-cells-3.html\u0022 rel=\u0022noopener\u0022\u003EProClone\u0026trade; Competent Cells\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/proclone-competent-cells-3.html\u0022 rel=\u0022noopener\u0022\u003EE003\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E1.2 ml (6 x 200 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E003\u0022 data-type=\u0022molecular\u0022\u003E$150.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/phi29-dna-polymerase-e014.html\u0022 rel=\u0022noopener\u0022\u003EUltra-Pure Phi29 DNA Polymerase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/phi29-dna-polymerase-e014.html\u0022 rel=\u0022noopener\u0022\u003EE014\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E014\u0022 data-type=\u0022molecular\u0022\u003E$152.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left\u0022 class=\u0022abm-page-menu only-hide\u0022 id=\u0022DNA-RNA-Applications\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000;\u0022\u003E\u003Cstrong\u003EDNA \u0026amp; RNA Applications\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnase-r-e049.html\u0022 rel=\u0022noopener\u0022\u003ERNase R\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnase-r-e049.html\u0022 rel=\u0022noopener\u0022\u003EE049\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E500 U (50 \u0026mu;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E049\u0022 data-type=\u0022molecular\u0022\u003E$310.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor.html\u0022 rel=\u0022noopener\u0022\u003ERNaseOFF Ribonuclease Inhibitor\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor.html\u0022 rel=\u0022noopener\u0022\u003EG138\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E4,000 U (100 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G138\u0022 data-type=\u0022molecular\u0022\u003E$80.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor-g591.html\u0022 rel=\u0022noopener\u0022\u003ERNaseOFF Ribonuclease Inhibitor\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor-g591.html\u0022 rel=\u0022noopener\u0022\u003EG591\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100,000 U (2 x 1.25 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G591\u0022 data-type=\u0022molecular\u0022\u003E$1400.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaser-rnaseoff-bundle.html\u0022 rel=\u0022noopener\u0022\u003ERNase R with RNaseOFF Bundle\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaser-rnaseoff-bundle.html\u0022 rel=\u0022noopener\u0022\u003EE049-G138S\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003EBundle\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E049-G138S\u0022 data-type=\u0022molecular\u0022\u003E$349.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnase-a-250.html\u0022 rel=\u0022noopener\u0022\u003ERNase A\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnase-a-250.html\u0022 rel=\u0022noopener\u0022\u003EG117\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E25 mg\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G117\u0022 data-type=\u0022molecular\u0022\u003E$57.50\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/dnase-i-g028.html\u0022 rel=\u0022noopener\u0022\u003EDNase I\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/dnase-i-g028.html\u0022 rel=\u0022noopener\u0022\u003EG028\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E2000 U (1.0 ml)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G028\u0022 data-type=\u0022molecular\u0022\u003E$89.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/dnase-i-rnase-free-e091.html\u0022 rel=\u0022noopener\u0022\u003EDNase I (RNase-Free)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/dnase-i-rnase-free-e091.html\u0022 rel=\u0022noopener\u0022\u003EE091\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E200 U (100 \u0026mu;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E091\u0022 data-type=\u0022molecular\u0022\u003E$99.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/poly-a-polymerase-yeast-e017.html\u0022 rel=\u0022noopener\u0022\u003EPoly(A) Polymerase, Yeast\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/poly-a-polymerase-yeast-e017.html\u0022 rel=\u0022noopener\u0022\u003EE017\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 U (100 \u0026mu;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E017\u0022 data-type=\u0022molecular\u0022\u003E$52.50\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/polyA-polymerase-e.coli-e099.html\u0022 rel=\u0022noopener\u0022\u003EPoly(A) Polymerase, \u003Cem\u003EE. coli\u003C\/em\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/polyA-polymerase-e.coli-e099.html\u0022 rel=\u0022noopener\u0022\u003EE099\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E25 U (25 \u0026micro;l)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E099\u0022 data-type=\u0022molecular\u0022\u003E$125.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/t7-rna-polymerase-e041.html\u0022 rel=\u0022noopener\u0022\u003ET7 RNA Polymerase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/t7-rna-polymerase-e041.html\u0022 rel=\u0022noopener\u0022\u003EE041\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 ul (5000 U)\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E041\u0022 data-type=\u0022molecular\u0022\u003E$68.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/t4-gene-32-protein-e040-vin.html\u0022 rel=\u0022noopener\u0022\u003ET4 Gene 32 Protein\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/t4-gene-32-protein-e040-vin.html\u0022 rel=\u0022noopener\u0022\u003EE040\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 \u0026mu;g\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E040\u0022 data-type=\u0022molecular\u0022\u003E$52.50\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/thermolabile-udg.html\u0022 rel=\u0022noopener\u0022\u003EThermolabile UDG\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/thermolabile-udg.html\u0022 rel=\u0022noopener\u0022\u003EE095\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E200 reactions\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E095\u0022 data-type=\u0022molecular\u0022\u003E$85.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-purified-protein-000033p.html\u0022 rel=\u0022noopener\u0022\u003EGFP Purified Protein\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-purified-protein-000033p.html\u0022 rel=\u0022noopener\u0022\u003E000033P\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E50 \u0026mu;g\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022000033P\u0022 data-type=\u0022molecular\u0022\u003E$158.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left\u0022 class=\u0022abm-page-menu only-hide\u0022 id=\u0022in-vitro-Kits-Proteases\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left;\u0022\u003E\u003Cspan style=\u0022color: #000;\u0022\u003E\u003Cstrong\u003EProteases\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/e027name.html\u0022 rel=\u0022noopener\u0022\u003ETEV Protease\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/e027name.html\u0022 rel=\u0022noopener\u0022\u003EE027\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 \u0026mu;l\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022E027\u0022 data-type=\u0022molecular\u0022\u003E$47.50\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/enterokinase-cleavage-enzyme-mammalian-produced-g699.html\u0022 rel=\u0022noopener\u0022\u003EEnterokinase Cleavage Enzyme (Mammalian Produced)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/enterokinase-cleavage-enzyme-mammalian-produced-g699.html\u0022 rel=\u0022noopener\u0022\u003EG699\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 Units\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G699\u0022 data-type=\u0022molecular\u0022\u003E$115.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left; width: 30.8635%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/proteinase-k-g029.html\u0022 rel=\u0022noopener\u0022\u003EProteinase K\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; width: 14.6197%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/proteinase-k-g029.html\u0022 rel=\u0022noopener\u0022\u003EG029\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left; width: 27.1058%;\u0022\u003E1.0 ml; 20mg\/ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G029\u0022\u003E$92.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Molecular Biology Enzymes and Kits","meta_keywords":"Molecular Biology Enzymes and Kits","meta_description":"Molecular Biology Enzymes and Kits","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":18410,"cat_no_base":"E087","parent_id":3195,"description":"\u003Cp\u003EThe Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability.\u0026nbsp;\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EKey Kit Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003E\u003Cstrong\u003EOne-step cloning:\u003C\/strong\u003E\u0026nbsp;No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EScar-free cloning:\u003C\/strong\u003E\u0026nbsp;No unwanted\/extra base pairs in the inserts\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EEasy-to-customize:\u003C\/strong\u003E\u0026nbsp;Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EReady for downstream applications:\u003C\/strong\u003E\u0026nbsp;Ligase-free reaction, no gel extraction or column purification steps needed\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003ERobust assembly:\u003C\/strong\u003E\u0026nbsp;Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly\u003C\/li\u003E\n\u003Cli\u003E\u003Cstrong\u003EIncreased thermostability:\u003C\/strong\u003E\u0026nbsp;High colony yields\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003E\u003Cstrong\u003ETestimonial:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E\u0022I tried the molecular cloning of inserting two DNA fragments (2Kb \u0026 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit.\u0022\u003C\/p\u003E\n\u003Cp\u003E-\u0026nbsp;\u003Cstrong\u003EJianfeng Lan\u003C\/strong\u003E, The Buck Institute\u003C\/p\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003ERapid multi-fragment cloning and assembly of whole vector constructs\u003C\/li\u003E\n\u003Cli\u003EClone in a promoter, gene, and tag, all in one step\u003C\/li\u003E\n\u003Cli\u003ERapid assembly of CRISPR multiplex gRNA vectors in one step\u003C\/li\u003E\n\u003C\/ul\u003E","components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":null,"product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":null,"note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2023-01-19 04:37:34","updated_at":"2025-02-24 23:54:21","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379519,"parent_id":3195,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/p\/r\/pro-ligation-free-cloning-kit-workflow.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18084862,"sku_in_m2":"E087","value_id_m2":49910119,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"},{"id":379520,"parent_id":3195,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/p\/r\/pro-ligation-free-cloning-kit-performance-data-e086-e087.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":18084862,"sku_in_m2":"E087","value_id_m2":49910120,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"},{"id":381288,"parent_id":3195,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/h\/q\/hqdefault_1_4.jpg","title":null,"text":null,"file_type":"video","alt":"One-Step Multi-fragment Cloning with the Pro Ligation-free Cloning Kit","url":"https:\/\/youtu.be\/sOjmMSHexW0","position":3,"status":0,"entity_id_m2":18084862,"sku_in_m2":"E087","value_id_m2":49910406,"attribute_id":0,"created_at":"2023-01-19 03:11:52","updated_at":"2024-05-16 21:14:59"}],"gene":null}