SNORD44 Primers
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Product Name
SNORD44 Primers -
Unit
150 µl (10 µM) of each Forward and Reverse Primers -
Description
SNORD44 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.
abm offers a comprehensive collection of miRNA qPCR primer sets which cover all known miRNA sequences. This qPCR primer set consists of a unique Forward Primer specific for the miRNA and a Universal 3’ miRNA Reverse Primer which can be used for qPCR expression analysis.
The Universal 3' miRNA Reverse Primer is compatible with miRNA cDNA synthesized using abm’s miRNA All-In-One cDNA Synthesis Kit. This reverse primer is specific for the unique and proprietary sequence incorporated into the miRNA cDNA by the Oligo d(T) with Adapter in the miRNA All-In-One cDNA Synthesis Kit. miRNA cDNA lacking this adapter sequence will not be compatible with this reverse primer.
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Gene Name
SNORD44 -
Accession Number
-
Species
Human (H. sapiens) -
System
qPCR Primers -
Insert Size
61 -
Note
Recommended Products for miRNA Expression Analysis:
Product Description miRNA All-In-One cDNA Synthesis Kit abm’s miRNA qPCR primers must be used with miRNA cDNA generated using abm’s miRNA All-In-One cDNA Synthesis Kit. abm’s miRNA qPCR primers and miRNA cDNA generated from abm’s miRNA All-In-One cDNA Synthesis Kit can be combined with BlasTaq™ qPCR MasterMix for miRNA detection and quantification.
This primer comes with each of abm’s miRNA qPCR Primer sets. It is also available separately for reorder.
U6 is a small nuclear RNA (snRNA) that is sometimes used as a reference gene in order to normalize miRNA expression levels in certain cell and tissue samples.
SNORD44 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.
SNORD48 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.
hsa-miR-16-5p is a miRNA that is often used as a reference gene in order to normalize miRNA expression levels.
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Supporting Protocol
Sen, A et al. "MicroRNA-138 regulates hypoxia-induced endothelial cell dysfunction by targeting S100A1" PLoS One 8(11):e78684 (2013). DOI: 10.1371/journal.pone.0078684. PubMed: 24244340.
Yasukochi, A., Kawakubo-Yasukochi, T., Morioka, M., Hazekawa, M., Nishinakagawa, T., Ono, K., ... & Nakamura, S. (2019). Regulation of collagen type XVII expression by miR203a-3p in oral squamous cell carcinoma cells. The Journal of Biochemistry. https://doi.org/10.1093/jb/mvz024
Question
ABM community
Verified customer
Asked on Mar 27 2025
Answer
The lentivirus should be aliquoted into smaller working volumes and then stored at -80°C. Lentivirus is sensitive to storage temperature and to freeze/thaws. It can lose up to 5% or more activity with each freeze/thaw. When stored properly, viral stocks of an appropriate titer should be suitable for use for up to one year. After long-term storage, we recommend re-titering your viral stocks before use.
ABM Scientific Support
Answered on Mar 27 2025
Question
ABM community
Verified customer
Asked on Jan 31 2023
Answer
Yes, it is ready to use. Just load suggested volume (5ul) to the well.
ABM Scientific Support
Answered on Jan 31 2023
ABM community
Verified customer
Asked on Feb 28 2025
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Feb 28 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Yes.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Optimal cell density for lentiviral transduction is generally 50-70% confluent for most cell types. Certain cell lines may require the optimal cell density to be determined experimentally.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Mar 24 2025
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SNORD44 Primers
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Current vector selected:
SNORD44 Primers
Cat. No.
MPH00003
Controls and Related Products
miRNA All-In-One cDNA Synthesis Kit
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20 rxn
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pLenti-III-mir-GFP Control Vector
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Lenti-III-mir-GFP Control Virus
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ViralEntry™ Transduction Enhancer (100X)
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src=\u0022\/assets\/images\/category\/rnai\/Resources_Intro-to-miRNA.png\u0022 alt=\u0022Introduction to miRNA\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EIntroduction to miRNA\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EmiRNA Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-3 col-md-3 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/miRNA-microRNA-detection\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/rnai\/Resources_miRNA-Detection.png\u0022 alt=\u0022miRNA Detection\u0022 width=\u0022100%\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EmiRNA Detection\u003C\/strong\u003E 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Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-3 col-md-3 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv\u003E\u003Ca href=\u0022\/assets\/catalog\/category\/docs\/v\/i\/Viral-Vectors-Brochure-Single-Pages.pdf\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/rnai\/Resources_Viral-Expression-Systems-Brochure.png\u0022 alt=\u0022Viral Expression Systems Brochure\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EViral Expression Systems Brochure\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EIntroductory Guide\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003ETop Publications\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #f25622;\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28356517\u0022 rel=\u0022noopener\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E01\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003EEpithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003EWu MJ. et al.\u003Cbr \/\u003ECell Metabolism (2019)\u003C\/i\u003E \u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #ef6331;\u0022 href=\u0022https:\/\/doi.org\/10.1016\/j.cmet.2018.11.004\u0022\u003Edoi: 10.1016\/j.cmet.2018.11.004\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #f25622;\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28534516\u0022 rel=\u0022noopener\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E02\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003EFibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003EZhou Y. et al.\u003Cbr \/\u003EScience Advances (2019)\u003C\/i\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #ef6331;\u0022 href=\u0022https:\/\/doi.org\/10.1126\/sciadv.aax0629\u0022\u003Edoi: 10.1126\/sciadv.aax0629\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-1 col-md-1\u0022 style=\u0022padding-left: 0px; padding-bottom: 20px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #f25622;\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28830450\u0022 rel=\u0022noopener\u0022\u003E\u003Cspan class=\u0022number-highlight\u0022\u003E03\u003C\/span\u003E\u003C\/a\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022 style=\u0022padding-left: 20px;\u0022\u003E\n\u003Cp\u003ECirculating miR-103a-3p contributes to angiotensin II-induced renal inflammation and fibrosis via a SNRK\/NF-\u0026kappa;B\/p65 regulatory axis.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Ci\u003ELu Q. et al.\u003Cbr \/\u003ENature Communications (2019)\u003C\/i\u003E\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022color: #ef6331;\u0022 href=\u0022https:\/\/doi.org\/10.1038\/s41467-019-10116-0\u0022\u003Edoi: 10.1038\/s41467-019-10116-0\u003C\/a\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cdiv class=\u0022col-sm-11 col-md-11\u0022\u003E\n\u003Cp\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript src=\u0022\/assets\/js\/abm.pages.pub.js\u0022\u003E\u003C\/script\u003E\n\u003Cscript\u003E\n window.addEventListener(\u0027load\u0027, () =\u003E {\n function getToken() {\n const el = document.head.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027);\n if (el) {\n return el.getAttribute(\u0027content\u0027) || \u0027\u0027;\n }\n return \u0027\u0027;\n }\n\n function getProducts(page = 1) {\n const category = document.getElementById(\u0027category-selector\u0027).value;\n const filter = document.getElementById(\u0027filter-selector\u0027).value;\n const query = document.getElementById(\u0027search-input\u0027).value;\n\n let loadingIndex = util.loading2(document.getElementById(\u0027outer-box\u0027));\n\n const searchData = {\n \u0027_token\u0027: getToken(),\n \u0027query\u0027: query,\n \u0027page\u0027: page,\n };\n\n if (\u0027utr\u0027 === filter) {\n searchData.filter_id = 9;\n }\n\n const expressionList = [\n \u0027Lenti Over-Expression\u0027,\n \u0027AAV Over-Expression\u0027,\n \u0027miRNA Synthetic Over-Expression\u0027,\n ];\n\n const inhibitorList = [\n \u0027Lenti Inhibitor\u0027,\n \u0027AAV Inhibitor\u0027,\n \u0027miRNA Synthetic Inhibitor\u0027,\n ];\n\n let mirnaType = \u0027\u0027;\n if (\u0027primer\u0027 === filter) {\n mirnaType = \u0027miRNA qPCR Primers\u0027;\n } else if (\u0027expression\u0027 === filter) {\n if (\u0027lenti\u0027 === category) {\n mirnaType = expressionList[0];\n } else if (\u0027aav\u0027 === category) {\n mirnaType = expressionList[1];\n } else if (\u0027syn\u0027 === category) {\n mirnaType = expressionList[2];\n } else if (\u0027all\u0027 === category) {\n mirnaType = expressionList.join(\u0027,\u0027);\n }\n } else if (\u0027inhibitor\u0027 === filter) {\n if (\u0027lenti\u0027 === category) {\n mirnaType = inhibitorList[0];\n } else if (\u0027aav\u0027 === category) {\n mirnaType = inhibitorList[1];\n } else if (\u0027syn\u0027 === category) {\n mirnaType = inhibitorList[2];\n } else if (\u0027all\u0027 === category) {\n mirnaType = inhibitorList.join(\u0027,\u0027);\n }\n }\n\n if (mirnaType) {\n searchData.mirna_type = mirnaType;\n }\n\n \/\/ Request data.\n fetch(\u0027\/product\/searchProducts\u0027, {\n method: \u0027POST\u0027,\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n },\n body: JSON.stringify(searchData)\n }).then(response =\u003E response.json()).then(response =\u003E {\n if (response.code === 0 \u0026\u0026 response.data) {\n renderProducts(response.data.products);\n renderPaginate(response.data.page, response.data.lastPage);\n }\n util.closeLoading2(loadingIndex);\n })\n }\n\n function generateContentItem(key, value) {\n let html = \u0027\u0027;\n if (value) {\n html += \u0027\u003Cli\u003E\u0027;\n html += \u0027\u003Cspan class=\u0022attribute\u0022\u003E\u0027 + key + \u0027:\u003C\/span\u003E\u0027;\n html += \u0027\u003Cspan class=\u0022attribute-value\u0022\u003E\u0027 + value + \u0027\u003C\/span\u003E\u0027;\n html += \u0027\u003C\/li\u003E\u0027;\n }\n\n return html;\n }\n\n function renderProducts(products) {\n let html = generateSearchResultSection(products);\n document.getElementById(\u0027result-list\u0027).innerHTML = html;\n loadVectorFrame();\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-section\u0027).innerHTML = html;\n\n document.querySelectorAll(\u0027#paginate-section a\u0027).forEach(el =\u003E {\n el.removeEventListener(\u0027click\u0027, handlePaginateClick);\n el.addEventListener(\u0027click\u0027, handlePaginateClick);\n });\n }\n\n function handlePaginateClick() {\n const page = this.dataset.page;\n if (this.parentElement.classList.contains(\u0027disabled\u0027)) {\n return false;\n }\n\n getProducts(page);\n }\n\n function displayFilterSelector() {\n const category = document.getElementById(\u0027category-selector\u0027).value;\n const selector = document.getElementById(\u0027filter-selector\u0027);\n selector.querySelectorAll(\u0027option\u0027).forEach(el =\u003E {\n el.removeAttribute(\u0027style\u0027);\n el.removeAttribute(\u0027selected\u0027);\n });\n\n function getOption(value) {\n return selector.querySelector(`option[value=\u0022${value}\u0022]`);\n }\n\n function hideOption(value) {\n const option = getOption(value);\n if (option) {\n option.style.display = \u0027none\u0027;\n }\n }\n\n function hideOptions(values) {\n for (const value of values) {\n hideOption(value);\n }\n }\n\n switch (category) {\n case \u0027qpcr\u0027:\n hideOptions([\u0027expression\u0027, \u0027inhibitor\u0027, \u0027utr\u0027]);\n break;\n case \u0027aav\u0027:\n case \u0027syn\u0027:\n hideOption(\u0027utr\u0027);\n case \u0027lenti\u0027:\n hideOption(\u0027primer\u0027);\n break;\n }\n\n const el = selector.querySelector(\u0027option:not([style])\u0027);\n if (el) {\n el.selected = \u0027selected\u0027;\n }\n }\n\n document.getElementById(\u0027category-selector\u0027).addEventListener(\u0027change\u0027, e =\u003E {\n e.preventDefault();\n\n displayFilterSelector();\n getProducts();\n });\n\n document.getElementById(\u0027search-button\u0027).addEventListener(\u0027click\u0027, e =\u003E {\n e.preventDefault();\n\n getProducts();\n });\n\n document.querySelector(\u0027#outer-box .ready-to-use-search-form\u0027).addEventListener(\u0027submit\u0027, e =\u003E {\n e.preventDefault();\n getProducts();\n return false;\n });\n });\n\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"miRNA","meta_keywords":"miRNA,microRNA,miRNA vector,miRNA expression vector,miRNA expression construct,miRNA expression virus,miRNA lentivirus,miRNA AAV,RNAi,RNA interference,premade miRNA vector,premade miRNA virus,miRNA inhibitor,miRNA agomir,miRNA antagomir,miRNA mimic,5\u0027 UTR miRNA reporter,3\u0027 UTR miRNA reporter,miRNA qPCR primers,premade miRNA vector,premade miRNA virus","meta_description":"abm offers an extensive collection of miRNA expression \u0026 inhibition vectors, synthetic inhibitors \u0026 mimics, and screening primers - all ready for your miRNA gene expression experiment.","deleted_at":null,"enable":"Y","parent_list":"8","table_name":"abm_catalog_genetic","image":null,"independentPage":0,"top_type":1,"sort_order":307,"in_footer":1,"fid":8,"created_at":null,"updated_at":"2025-04-07 07:57:56"},"info":{"id":1557634,"parent_id":1964307,"description":"\u003Cp\u003ESNORD44 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a comprehensive collection of miRNA qPCR primer sets which cover all known miRNA sequences. \u0026nbsp;This qPCR primer set consists of a unique Forward Primer specific for the miRNA and a \u003Ca href=\u0022\/Universal-3-miRNA-Reverse-Primer-mph00000.html\u0022\u003EUniversal 3\u0026rsquo; miRNA Reverse Primer\u003C\/a\u003E which can be used for qPCR expression analysis.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003EThe Universal 3\u0027 miRNA Reverse Primer is compatible with miRNA cDNA synthesized using \u003Cstrong\u003Eabm\u0026rsquo;s\u003C\/strong\u003E \u003Ca href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E. This reverse primer is specific for the unique and proprietary sequence incorporated into the miRNA cDNA by the Oligo d(T) with Adapter in the miRNA All-In-One cDNA Synthesis Kit.\u0026nbsp; miRNA cDNA lacking this adapter sequence will not be compatible with this reverse primer.\u003C\/p\u003E","alias":null,"list_of_species":null,"vector":null,"titer":null,"cell_type":null,"application":"\u003Ctable id=\u0022product-attribute-specs-table\u0022 class=\u0022data table additional-attributes product-attribute-specs-table\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr class=\u0022configProduct\u0022\u003E\n\u003Ctd id=\u0022prod-Note\u0022 class=\u0022col data\u0022 data-th=\u0022Note\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u0026nbsp;\u003Ca class=\u0022product-item-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html#G898\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u0026nbsp;(Cat No. G898)\u0026nbsp;must be used to reverse transcribe the RNA. Failure to do so will not yield valid qPCR results. Each primer set includes a universal reverse primer and is specific for the unique and proprietary sequence incorporated into the cDNA by the miRNA adapter in the miRNA cDNA synthesis kit. The miRNA qPCR primer sets cannot accurately detect cDNA generated using other first strand synthesis kits.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","system":"qPCR Primers","insert_size":"61","list_of_markers":null,"alt_accession_number":null,"previous_gene_symbol":null,"promoter":null,"tag":null,"target_sequence":null,"serotype":null,"hgnc_id":null,"appearance":null,"vector_size":null,"growth_properties":null,"morphology":null,"chromosome_location":null,"concentration":null,"sequencing_primers":null,"components":null,"conversion_of_od":null,"chemical_modification":null,"format":null,"protein_type":null,"mirna_url":null,"protein_family":null,"protein_function":null,"purification_method":null,"source_catno":null,"source_price":null,"source_text":null,"expressed_region_text":null,"swiss_prot_text":null,"molecular_weight":null,"endotoxin_level":null,"purity":null,"biological_activity_text":null,"oos_message":null,"storage_buffer":null,"storage_condition":null,"growth_medium":null,"propagation_method":null,"shelf_life":null,"qc":null,"shipping_conditions":null,"note":"\u003Cp\u003E\u003Cspan style=\u0022font-size: 12pt;\u0022\u003E\u003Cstrong\u003ERecommended Products for miRNA Expression Analysis:\u003C\/strong\u003E\u003C\/span\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022border-collapse: collapse; width: 100%; height: 193px; border-color: #ced4d9; border-style: solid;\u0022 border=\u00221px\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Cth style=\u0022width: 31.7638%; height: 44px;\u0022\u003E\u003Cstrong\u003EProduct\u003C\/strong\u003E\u003Cspan style=\u0022font-size: 8pt;\u0022\u003E\u0026nbsp;\u003C\/span\u003E\u003C\/th\u003E\n\u003Cth style=\u0022width: 65.7702%; height: 44px;\u0022\u003E\u003Cstrong\u003EDescription\u003C\/strong\u003E\u003Cspan style=\u0022font-size: 8pt;\u0022\u003E\u0026nbsp;\u003C\/span\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 41px;\u0022\u003E\u003Ca href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit \u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 41px;\u0022\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s miRNA qPCR primers must be used \u0026nbsp;with miRNA cDNA generated using \u003Cstrong\u003Eabm\u0026rsquo;s \u003C\/strong\u003EmiRNA All-In-One cDNA Synthesis Kit.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 10px;\u0022\u003E\n\u003Cp\u003E\u003Ca title=\u0022BlasTaq\u0026trade; qPCR MasterMix\u0022 href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022\u003EBlasTaq\u0026trade; qPCR MasterMix \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 10px;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s miRNA qPCR primers and miRNA cDNA generated from \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s miRNA All-In-One cDNA Synthesis Kit can be combined with BlasTaq\u0026trade; qPCR MasterMix for miRNA detection and quantification.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 22px;\u0022\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/Universal-3-miRNA-Reverse-Primer-mph00000.html\u0022\u003EUniversal 3\u0026rsquo; miRNA Reverse Primer \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 22px;\u0022\u003E\n\u003Cp\u003EThis primer comes with each of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s miRNA qPCR Primer sets. It is also available separately for reorder.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 22px;\u0022\u003E\n\u003Cp\u003E\u003Ca title=\u0022U6 Primers\u0022 href=\u0022\/U6-2-Primers-mph00001.html\u0022\u003EU6 Primers \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 22px;\u0022\u003E\n\u003Cp\u003EU6 is a small nuclear RNA (snRNA) that is sometimes used as a reference gene in order to normalize miRNA expression levels in certain cell and tissue samples.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 22px;\u0022\u003E\n\u003Cp\u003E\u003Ca title=\u0022SNORD44 Primers\u0022 href=\u0022\/SNORD44-Primers-MPH00003.html\u0022\u003ESNORD44 Primers \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 22px;\u0022\u003E\n\u003Cp\u003ESNORD44 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 10px;\u0022\u003E\n\u003Cp\u003E\u003Ca title=\u0022SNORD48 Primers\u0022 href=\u0022\/SNORD48-Primers-MPH00005.html\u0022\u003ESNORD48 Primers \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 10px;\u0022\u003E\n\u003Cp\u003ESNORD48 is a small nucleolar RNA (snoRNA) that is often used as a reference gene in order to normalize miRNA expression levels.\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 31.7638%; height: 22px;\u0022\u003E\n\u003Cp\u003E\u003Ca title=\u0022hsa-miR-16-5p Primers\u0022 href=\u0022\/hsa-miR-16-5p-Primers-mph02234.html\u0022\u003Ehsa-miR-16-5p Primers \u003C\/a\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003Ctd style=\u0022width: 65.7702%; height: 22px;\u0022\u003Ehsa-miR-16-5p is a miRNA that is often used as a reference gene in order to normalize miRNA expression levels.\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","note_internal":null,"reconstitution":null,"cloning_sites":null,"insert_sequence":null,"inventory_location":null,"lead_time":null,"temp_lead_time":null,"library":null,"qty":null,"supplier":null,"sn":null,"csn":null,"ssn":null,"dsn":null,"species":null,"species_int":null,"features":null,"features_int":null,"vector_virus_cell":null,"vector_virus_cell_int":null,"disclaimer":null,"guarantee":null,"online":0,"subculture_protocol":null,"caution":null,"recommend":null,"associated_diseases":null,"pmid":null,"created_at":"2023-06-15 21:18:11","updated_at":"2025-03-04 18:02:43","originating_source":null,"product_quote":0,"tmp":null,"disclaimer_general":null,"promotions":null},"circ_rna":null,"datasheet_options":[],"seo":{"id":1192102,"catalog_id":1964307,"catalog_type":"App\\Models\\CatalogBaseLibrary","url_key":"SNORD44-Primers-MPH00003.html","meta_title":null,"meta_keywords":null,"meta_description":null,"created_at":"2024-01-16 00:28:39","updated_at":"2024-05-13 23:16:22"},"gene":null}