miRNA All-In-One cDNA Synthesis Kit
Cat. No.
G898
Unit
20 rxn
Price
$185.00
| Cat. No. | G898 | ||||||||
| Name | miRNA All-In-One cDNA Synthesis Kit | ||||||||
| Unit | 20 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
The miRNA All-In-One cDNA Synthesis Kit provides a comprehensive solution for efficient synthesis of first-strand miRNA cDNA from RNA samples. The kit includes all necessary components for a hassle-free setup, enabling RNA molecules to undergo polyadenylation and reverse transcription in a simple, single-tube reaction. This process uses Poly(A) Polymerase, OneScript® Plus Reverse Transcriptase, and a modified Oligo d(T) with Adapter that anneals to poly(A)-tailed miRNA. The included SuperMix contains RNaseOFF Ribonuclease Inhibitor to protect RNA from degradation.
The synthesized first-strand miRNA cDNA can be directly used as a template for qPCR-based miRNA expression analysis with BlasTaq™ qPCR MasterMix (sold separately, Cat. No. G891). Users can choose from abm’s extensive collection of miRNA qPCR primers, covering all known miRNA sequences. Each primer set includes a unique miRNA Forward Primer and a Universal 3' miRNA Reverse Primer that binds to the complementary Adapter sequence on the modified Oligo d(T).
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G898 |
Datasheet
| Can I use your miRNA cDNA synthesis kit for quantifying mature miRNAs with qPCR? | |
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Yes, the miRNA All-In-One cDNA Synthesis Kit is designed for synthesizing cDNA from miRNAs, which can be directly used for qPCR-based analysis. Pair with BlasTaq™ qPCR MasterMix (sold separately, Cat. No. G891) and the appropriate miRNA forward primer from our miRNA qPCR Primer Library.
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| What RNA samples are suitable for use with the miRNA cDNA Synthesis Kit? | |
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Both small RNA (200 ng) and total RNA (up to 2 μg) can be used. Small RNA typically yields better results, but both types are compatible with the kit.
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| How do I protect RNA integrity during the cDNA synthesis process? | |
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The kit includes RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation during the cDNA synthesis process.
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| What is the role of the modified Oligo d(T) with Adapter in the cDNA synthesis process? | |
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The modified Oligo d(T) with Adapter binds initiates polyA-tailing and reverse transcription of the mature miRNAs enabling the synthesis of cDNA for subsequent analysis. The adapter contains the Universal reverse sequence enabling easy quantification through real-time PCR.
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| Can I use my own primers for qPCR with this kit? | |
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The kit is optimized for use with our miRNA primers, but other primers compatible with the Universal 3' miRNA Reverse Primer may also work. For optimal results, we recommend using our forward miRNA primers found in the miRNA qPCR Primer Library.
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| Are custom miRNA primers available for miRNAs not listed on your website? | |
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Yes, we offer custom primer design services for miRNAs not currently available in our catalog. Contact technical@abmgood.com with the miRNA accession numbers, and we'll be happy to design the primers for you. |
| How should I store the synthesized cDNA for long-term use? | |
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The synthesized miRNA cDNA can be stored at -20°C for long-term use without compromising its quality.
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| Do you provide housekeeping gene primers for use as internal controls in miRNA qPCR? | |
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Yes, we offer various housekeeping gene primers, including U6 (Cat. No. MPH00001), SNORD44 (Cat. No. MPH00003), and SNORD48 (Cat. No. MPH00005), which can be used as internal controls in qPCR.
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| What is the miRBase Sequence Database, and how can it help with miRNA research? | |
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miRBase is a comprehensive database of predicted and validated miRNA sequences, providing unique identifiers for miRNAs and searchable information about miRNA sequences and their target genes.
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| Do you offer in situ miRNA detection methods or mutant constructs for miRNA-mRNA interaction studies? | |
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We do not offer in situ miRNA detection methods or pre-made mutant constructs, but we can create custom mutant constructs for 3'UTR miRNA reporter vectors. Contact techinical@abmgood.com for custom services.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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Wu, G., Ju, X., Wang, Y., Li, Z., & Gan, X. (2019). Up-regulation of SNHG6 activates SERPINH1 expression by competitive binding to miR-139-5p to promote hepatocellular carcinoma progression. Cell Cycle, 18(16), 1849-1867. https://doi.org/10.1080/15384101.2019.1629772
Wu, G., Ma, L., Wang, L., Zhou, J., Ma, Y., & Yang, C. (2020). Analysis of transcriptome and miRNAome in the muscle of bamei pigs at different developmental stages. Animals, 10(7), 1198. https://doi.org/10.3390/ani10071198
Wu, L., Chang, L., Wang, H., Ma, W., Peng, Q., & Yuan, Y. (2018). Clinical significance of C/D box small nucleolar RNA U76 as an oncogene and a prognostic biomarker in hepatocellular carcinoma. Clinics and Research in Hepatology and Gastroenterology, 42(1), 82-91. https://doi.org/10.1016/j.clinre.2017.04.018
Xiao, Y., Najeeb, R. M., Ma, D., Yang, K., Zhong, Q., & Liu, Q. (2019). Upregulation of CENPM promotes hepatocarcinogenesis through mutiple mechanisms. Journal of Experimental & Clinical Cancer Research, 38, 1-13. https://doi.org/10.1186/s13046-019-1444-0
Xiong, X., Sun, D., Chai, H., Shan, W., Yu, Y., Pu, L., & Cheng, F. (2015). MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma. Biochemical and biophysical research communications, 465(2), 262-269. https://doi.org/10.1016/j.bbrc.2015.08.013
Yan, Y., Tian, L. Y., Jia, Q., Han, Y., Tian, Y., Chen, H. N., ... & Zhao, X. J. (2023). MiR-130a-3p regulates FUNDC1-mediated mitophagy by targeting GJA1 in myocardial ischemia/reperfusion injury. Cell Death Discovery, 9(1), 77. https://doi.org/10.1038/s41420-023-01372-7
Yaren, A., Gökdeniz Yıldırım, A., Demiray, A., Koç, A. C., & Şenol, H. (2023). Association between 20 serum mirnas and clinicopathological variables in patients with breast cancer. Pamukkale Tıp Dergisi. https://hdl.handle.net/11499/51434
Yea, J. H., Yoon, Y. M., Lee, J. H., Yun, C. W., & Lee, S. H. (2021). Exosomes isolated from melatonin-stimulated mesenchymal stem cells improve kidney function by regulating inflammation and fibrosis in a chronic kidney disease mouse model. Journal of Tissue Engineering, 12, 20417314211059624. https://doi.org/10.1177/20417314211059624
Yildirim, A. G., Demiray, A., Koc, A. C., Senol, H., & Yaren, A. (2023). Association between 20 serum mirnas and clinicopathological variables in patients with breast cancer/Meme kanserli hastalarda 20 serum mirna'nin klinikopatolojik degiskenler ile iliskisi. Pamukkale Medical Journal, 16(2), 168-179. https://doi.org/10.31362/patd.1216451
Yıldırım, O., Seçme, M., Dodurga, Y., Mete, G. A., & Fenkci, S. M. (2024). Effects of boric acid on proliferation, apoptosis and miRNAs in medullary thyroid cancer cells. https://doi.org/10.21203/rs.3.rs-3986243/v1
Zapała, B., Kamińska, A., Piwowar, M., Paziewska, A., Gala-Błądzińska, A., & Stępień, E. Ł. (2023). miRNA signature of urine extracellular vesicles shows the involvement of inflammatory and apoptotic processes in diabetic chronic kidney disease. Pharmaceutical research, 40(4), 817-832. https://doi.org/10.1007/s11095-023-03481-5
Zeng, Q., Jin, F., Qian, H., Chen, H., Wang, Y., Zhang, D., ... & Chai, C. (2022). The miR-345-3p/PPP2CA signaling axis promotes proliferation and invasion of breast cancer cells. Carcinogenesis, 43(2), 150-159. https://doi.org/10.1093/carcin/bgab124
Zhang, X., Pan, B., Qiu, J., Ke, X., Shen, S., Wang, X., & Tang, N. (2022). lncRNA MIAT targets miR‐411‐5p/STAT3/PD‐L1 axis mediating hepatocellular carcinoma immune response. International Journal of Experimental Pathology, 103(3), 102-111. https://doi.org/10.1111/iep.12440
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Simply add your RNA sample with our mastermix and incubate.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-workflow.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EAccurate cDNA Synthesis\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EGenomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-figure.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Hot RT\u0022 id=\u0022OneScriptHot\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ESynthesize cDNA from complex RNA templates with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase. Engineered for outstanding thermostability, our OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/extreme-thermostability-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EExtreme thermostability at 50-80\u0026deg;C\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/transcribes-degraded-contaminated-samples-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003ETranscribes degraded samples and resists inhibitors \/ contaminants\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/high-cDNA-yields-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EHigh cDNA yields from difficult or ultra-low RNA samples\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-onescript-hot-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022--first-child-width: 50%;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EOneScript\u0026reg; Hot cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EG594\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G594\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EOneScript\u0026reg; Hot Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EG593\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G593\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EMegaFi\u0026trade; One-Step RT-PCR\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EG597\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G597\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EOutstanding Thermostability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-hot-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase has exceptional thermostability. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase (Cat. No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80\u02daC. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Plus RT\u0022 id=\u0022OneScriptPlus\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ERoutine cDNA synthesis can be completed in just 10-15 minutes with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase! 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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-reverse-transcriptase.html\u0022\u003EOneScript\u0026reg; Plus Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-mmlv-reverse-transcriptase.html\u0022\u003EG237\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G237\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EOneScript\u0026reg; Plus cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EG236\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G236\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent Elongation Ability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-plus-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (Cat. No. G237) was used in a reaction with a range of human RNA fragments. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUnsure which reverse transcriptase is right for you?\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which reverse transcriptase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple RT Enzyme Selection Guide below to compare the characteristics of each reverse transcriptase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth width=\u002240%\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptHot\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptPlus\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus\u003C\/a\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EThermostability\u003C\/td\u003E\n\u003Ctd\u003E50-80\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50-55\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOptimal Temperature\u003C\/td\u003E\n\u003Ctd\u003E60\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMaximum cDNA Elongation\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMinimum Sample Input\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EReaction Time\u003C\/td\u003E\n\u003Ctd\u003E10 min\u003C\/td\u003E\n\u003Ctd\u003E15 min\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EInhibitor\/Contamination Resistance\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETolerance to Complex Templates\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESee for yourself why your fellow RT-PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1400px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-variations\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-Variations-to-the-system_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EVariations to the System\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv 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{\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/div\u003E","meta_title":"Reverse Transcription \u0026 RT-PCR","meta_keywords":"Reverse Transcription \u0026 RT-PCR","meta_description":"Reverse Transcription \u0026 RT-PCR","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":103,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":912,"cat_no_base":"G898","parent_id":116,"description":"\u003Cdiv class=\u0022flex-shrink-0 flex flex-col relative items-end\u0022\u003E\n\u003Cdiv class=\u0022pt-0\u0022\u003E\n\u003Cdiv class=\u0022gizmo-bot-avatar flex h-8 w-8 items-center justify-center overflow-hidden rounded-full\u0022\u003E\n\u003Cdiv class=\u0022relative p-1 rounded-sm flex items-center justify-center bg-token-main-surface-primary text-token-text-primary h-8 w-8\u0022\u003E\n\u003Cdiv class=\u0022flex-shrink-0 flex flex-col relative items-end\u0022\u003E\n\u003Cdiv\u003E\n\u003Cdiv class=\u0022pt-0\u0022\u003E\n\u003Cdiv class=\u0022gizmo-bot-avatar flex h-8 w-8 items-center justify-center overflow-hidden rounded-full\u0022\u003E\n\u003Cdiv class=\u0022relative p-1 rounded-sm flex items-center justify-center bg-token-main-surface-primary text-token-text-primary h-8 w-8\u0022\u003EThe \u003Cstrong\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/strong\u003E provides a comprehensive solution for efficient synthesis of first-strand miRNA cDNA from RNA samples. The kit includes all necessary components for a \u003Cstrong\u003Ehassle-free setup\u003C\/strong\u003E, enabling RNA molecules to undergo polyadenylation and reverse transcription in a \u003Cstrong\u003Esimple, single-tube reaction\u003C\/strong\u003E. This process uses Poly(A) Polymerase, OneScript\u0026reg; Plus Reverse Transcriptase, and a modified Oligo d(T) with Adapter that anneals to poly(A)-tailed miRNA. The included SuperMix contains RNaseOFF Ribonuclease Inhibitor to protect RNA from degradation.\u003C\/div\u003E\n\u003Cdiv class=\u0022relative p-1 rounded-sm flex items-center justify-center bg-token-main-surface-primary text-token-text-primary h-8 w-8\u0022\u003E\u0026nbsp;\u003C\/div\u003E\n\u003Cdiv class=\u0022relative p-1 rounded-sm flex items-center justify-center bg-token-main-surface-primary text-token-text-primary h-8 w-8\u0022\u003EThe synthesized first-strand miRNA cDNA can be directly used as a template for qPCR-based miRNA expression analysis with \u003Cstrong\u003EBlasTaq\u0026trade; qPCR MasterMix\u003C\/strong\u003E (sold separately, \u003Ca href=\u0022\/blastaq-2x-qpcr-mastermix.html\u0022\u003ECat. No. G891\u003C\/a\u003E). Users can choose from \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s extensive collection of \u003Ca href=\u0022\/mirna-qpcr-primer-library.html\u0022\u003EmiRNA qPCR primers\u003C\/a\u003E, covering all known miRNA sequences. Each primer set includes a unique miRNA Forward Primer and a Universal 3\u0027 miRNA Reverse Primer that binds to the complementary Adapter sequence on the modified Oligo d(T).\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E2X miRNA cDNA Synthesis SuperMix\u003C\/td\u003E\n\u003Ctd\u003E20 rxn (200 \u0026mu;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EEnzyme Mix\u003C\/td\u003E\n\u003Ctd\u003E20 rxn (40 \u0026mu;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENuclease-Free H\u003Csub\u003E2\u003C\/sub\u003EO\u003C\/td\u003E\n\u003Ctd\u003E1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp align=\u0022left\u0022\u003EStore at -20\u0026deg;C.\u0026nbsp;This product is stable for 2 years from the date of shipping if stored and handled properly.\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":"PCR-2A","note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":null,"updated_at":"2025-01-30 22:07:18","short_description":"\u003Cp\u003EmiRNA All-In-One cDNA Synthesis Kit\u00a0is a complete system for\u00a0efficient synthesis of first strand miRNA from total RNA templates.\u00a0\u003C\/p\u003E","reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":380815,"parent_id":116,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/g\/e\/generic-kit-photo_3.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18124982,"sku_in_m2":"G898","value_id_m2":49911851,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:39:19"},{"id":388468,"parent_id":116,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/tMwA6mVX5QKCe4p6yDJNkPzUBtoxnqiIoYVCPHFe.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-18 22:14:54","updated_at":"2025-02-18 22:15:03"},{"id":388292,"parent_id":116,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/kdfFzb0OMOZ5U5zO94vvmKNug4QTbvNLxYxXNUXq.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-02-19 21:45:37","updated_at":"2025-02-19 21:48:03"}],"gene":null}