OneScript® Hot cDNA Synthesis Kit
Cat. No.
G594
Unit
100 rxn
Price
$207.00
| Cat. No. | G594 | ||||||||||||||||
| Name | OneScript® Hot cDNA Synthesis Kit | ||||||||||||||||
| Unit | 100 rxn | ||||||||||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||||||||||
| Description |
OneScript® Hot cDNA Synthesis Kit is the first kit in the world that allows for efficient and robust cDNA synthesis at 60ºC. All other cDNA synthesis kits on the market are optimized for the reaction to be performed at 42-55ºC, which is sufficient for most RNA template, but fails on more difficult templates. abm is the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its incredible thermostability at 60-72ºC. The enzyme is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors. OneScript® Hot is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G594 |
Datasheet
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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| Can I synthesize cDNA from long RNA transcripts? | |
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Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
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| What can I use the synthesized cDNA for? | |
|
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
|
Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
|
We recommend using 1 ng to 2 μg of RNA per reaction.
|
| What volumes of cDNA should I use for downstream PCR? | |
|
Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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What if input RNA samples are expected to have high levels of genomic DNA contamination?
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If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
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| What is the advantage of OneScript® Hot Reverse Transcriptase compared to OneScript® Plus Reverse Transcriptase? | |
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OneScript® Hot Reverse Transcriptase offers a significant advantage over enzymes that operate at lower temperatures (42-55ºC) due to its exceptional thermostability, allowing it to function effectively at 60-72ºC. This higher temperature improves efficiency by preventing RNA secondary structures and enabling cDNA synthesis from degraded or low-abundance RNA. Additionally, it is more resistant to inhibitors and contaminants, making it ideal for complex or impure RNA samples.
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Do I need an RNase inhibitor when using this product?
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All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
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Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003E\u003Cspan style=\u0022text-decoration: line-through;\u0022\u003E$209.00\u003C\/span\u003E $189.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal*\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022font-weight: normal; text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022 rel=\u0022noopener\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003E$209.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022\u003E\n\u003Ctd style=\u0022text-align: left;\u0022 colspan=\u00224\u0022\u003E\u003Cspan style=\u0022color: #00000;\u0022\u003E\u003Cstrong\u003EcDNA Synthesis Kit \u003C\/strong\u003E\u003Cem\u003E(Enzyme with RNaseOFF, Buffer, dNTPs, Oligo(dT), Random Primers; 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This optimized solution includes \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s proprietary OneScript\u0026reg; Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a perfectly balanced mix of Oligo (dT)s and Random Primers, all in ONE convenient tube!\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003EFor applications using pure RNA where genomic DNA removal is not required, try our \u003Cstrong\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/strong\u003E. It offers a faster protocol for an even more efficient cDNA synthesis.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/genomoic-dna-removal.png\u0022 \/\u003E\n\u003Cp\u003EGenomic DNA Removal*\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/efficient-cdna-synthesis.png\u0022 \/\u003E\n\u003Cp\u003EEfficient cDNA Synthesis\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/easy-one-step-setup.png\u0022 \/\u003E\n\u003Cp\u003EEasy One-Step Setup\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal*\u003C\/a\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Simple\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/strong\u003E is a convenient and ready-to-use formulation for first-strand cDNA synthesis with an easy one-step setup that reduces pipetting and sample handling. Simply add your RNA sample with our mastermix and incubate.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-workflow.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EAccurate cDNA Synthesis\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EGenomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-figure.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Hot RT\u0022 id=\u0022OneScriptHot\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ESynthesize cDNA from complex RNA templates with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase. 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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-reverse-transcriptase.html\u0022\u003EOneScript\u0026reg; Plus Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-mmlv-reverse-transcriptase.html\u0022\u003EG237\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G237\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EOneScript\u0026reg; Plus cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EG236\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G236\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent Elongation Ability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-plus-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (Cat. No. G237) was used in a reaction with a range of human RNA fragments. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUnsure which reverse transcriptase is right for you?\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which reverse transcriptase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple RT Enzyme Selection Guide below to compare the characteristics of each reverse transcriptase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth width=\u002240%\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptHot\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptPlus\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus\u003C\/a\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EThermostability\u003C\/td\u003E\n\u003Ctd\u003E50-80\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50-55\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOptimal Temperature\u003C\/td\u003E\n\u003Ctd\u003E60\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMaximum cDNA Elongation\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMinimum Sample Input\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EReaction Time\u003C\/td\u003E\n\u003Ctd\u003E10 min\u003C\/td\u003E\n\u003Ctd\u003E15 min\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EInhibitor\/Contamination Resistance\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETolerance to Complex Templates\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESee for yourself why your fellow RT-PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1400px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-variations\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-Variations-to-the-system_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EVariations to the System\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/div\u003E","meta_title":"Reverse Transcription \u0026 RT-PCR","meta_keywords":"Reverse Transcription \u0026 RT-PCR","meta_description":"Reverse Transcription \u0026 RT-PCR","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":103,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":16607,"cat_no_base":"G594","parent_id":713,"description":"\u003Cp\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot cDNA Synthesis Kit\u003C\/strong\u003E is the first kit in the world that allows for efficient and robust cDNA synthesis at 60\u0026ordm;C. All other cDNA synthesis kits on the market are optimized for the reaction to be performed at 42-55\u0026ordm;C, which is sufficient for most RNA template, but fails on more difficult templates. \u003Cstrong\u003Eabm \u003C\/strong\u003Eis the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its\u003Cstrong\u003E incredible thermostability at 60-72\u0026ordm;C.\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003EThe enzyme is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase, that can reverse transcribe low abundance or degraded RNA, and has significantly better resistance to contaminating inhibitors such as reagents used during RNA extraction and contaminants from biological samples. High processivity and sensitivity allow for rapid cDNA synthesis of full-length cDNA fragments in a fraction of the time of leading competitors.\u0026nbsp;OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot is formulated with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (\u003C 1 mM), making it the best choice for ultimate RNA protection.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EExtremely thermostable cDNA synthesis at 60-72\u0026ordm;C\u003C\/li\u003E\n\u003Cli\u003ETranscribes degraded samples and resists inhibitors\/contaminants\u003C\/li\u003E\n\u003Cli\u003EHigh cDNA yields from difficult or ultra-low RNA samples\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp style=\u0022margin: 30px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase\u003C\/td\u003E\n\u003Ctd\u003E100 rxn (100 \u0026micro;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E5X RT Buffer\u003C\/td\u003E\n\u003Ctd\u003E400 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOligo(dT) (10 \u0026mu;M)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ERandom Primers (10 \u0026mu;M)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EdNTP (10 mM)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENuclease-Free H\u003Csub\u003E2\u003C\/sub\u003EO\u003C\/td\u003E\n\u003Ctd\u003E2 x 1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; 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