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General Materials

OneScript® Plus cDNA Synthesis Kit

Cat. No.

G236

Unit

100 rxn

Price

$212.00

Cat. No.

G236

Name

OneScript® Plus cDNA Synthesis Kit

Unit

100 rxn

Category

Reverse Transcriptase & RT-PCR

Description

OneScript^® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor, offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features

Maximal flexibility in priming – oligo(dT), random primers, or gene-specific primers
Robust cDNA synthesis from any RNA template
High reproducibility and excellent yield
Employs abm's OneScript^® Plus Reverse Transcriptase (Cat. No. G237)
Included in abm's PCR Buffet Program

Product Component	Quantity
5X RT Buffer	400 µl
Oligo(dT) (10 μM)	100 µl
Random Primers (10 μM)	100 µl
dNTP (10 mM)	100 µl
Nuclease-Free H₂O	2 x 1.0 ml
OneScript^® Plus Reverse Transcriptase	100 rxn (100 µl)

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Application

Synthesizing cDNA from ssRNA
DNA primer extension
Sequencing dsDNA
Constructing cDNA library
Producing template for use in RT-PCR
Labelling 3'-end of duplex DNA via end-filling reactions
Generating probes for hybridization

Storage Condition

Store at -20°C.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G236

Datasheet

G236 OneScript® Plus cDNA Synthesis Kit Datasheet

Search CoA here

MSDS

G236 MSDS

Supporting Protocol

Other

PCR Product Guide

	Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis?
	Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.

	What can I use the synthesized cDNA for?
	The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.

	How should I store the synthesized cDNA?
	Store the synthesized first-strand cDNA at -20°C for long-term use.

Why am I getting low cDNA yields?

Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:

Check RNA integrity with gel electrophoresis or a BioAnalyzer, aiming for a minimum A260/A280 ratio of 1.7 or a RIN above 8.
Clean the RNA using ethanol precipitation or lithium chloride to remove contaminants.
Purify the RNA with extraction kits or phenol/chloroform to remove proteins.
Increase RNA input, especially for low-abundance samples.

	How much RNA template should I use for cDNA synthesis?
	We recommend using 1 ng to 2 μg of RNA per reaction.

	What volumes of cDNA should I use for downstream PCR?
	Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.

	What if input RNA samples are expected to have high levels of genomic DNA contamination?
	If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).

	Do I need an RNase inhibitor when using this product?
	All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.

	What discontinued abm products is Cat. No. G236 equivalent to?
	This product is abm’s next generation of cDNA Synthesis Kits and is functionally equivalent to Cat. No. G233, G234, G235, and G491 (OneScript™ and OneScript™ Plus cDNA Synthesis Kits), with improved performance. Contact our customer service team at technical@abmgood.com for more information.

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text-align: left;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022 rel=\u0022noopener\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022text-align: left;\u0022\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003E$180.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022RT MasterMixes\u0022 id=\u0022AllInOne\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERT MasterMixes\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003EEasily synthesize cDNA in a single step with our \u003Cstrong\u003EAll-In-One 5X RT MasterMix with gDNA Removal*\u003C\/strong\u003E. This optimized solution includes \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s proprietary OneScript\u0026reg; Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a perfectly balanced mix of Oligo (dT)s and Random Primers, all in ONE convenient tube!\u003C\/p\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003EFor applications using pure RNA where genomic DNA removal is not required, try our \u003Cstrong\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/strong\u003E. 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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal*\u003C\/a\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003ESuper Simple\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/strong\u003E is a convenient and ready-to-use formulation for first-strand cDNA synthesis with an easy one-step setup that reduces pipetting and sample handling. 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No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80\u02daC. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Plus RT\u0022 id=\u0022OneScriptPlus\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ERoutine cDNA synthesis can be completed in just 10-15 minutes with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase! OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase has improved thermostability and is engineered for high cDNA yields, high efficiency on low-input, long, or difficult RNA templates, and resistance to inhibitors and contaminants.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/high-thermostability-icon.png\u0022 \/\u003E\n\u003Cp\u003EHigh thermostability at 50-55\u0026deg;C\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/synthesize-long-templates-icon.png\u0022 \/\u003E\n\u003Cp\u003ESynthesize 15 kb RNA Templates\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/10-15-min-reaction-time-icon.png\u0022 \/\u003E\n\u003Cp\u003E10-15 minutes reaction time\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-onescript-plus-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-reverse-transcriptase.html\u0022\u003EOneScript\u0026reg; Plus Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-mmlv-reverse-transcriptase.html\u0022\u003EG237\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G237\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EOneScript\u0026reg; Plus cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EG236\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G236\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent Elongation Ability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-plus-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (Cat. No. G237) was used in a reaction with a range of human RNA fragments. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUnsure which reverse transcriptase is right for you?\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which reverse transcriptase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple RT Enzyme Selection Guide below to compare the characteristics of each reverse transcriptase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth width=\u002240%\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptHot\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptPlus\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus\u003C\/a\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EThermostability\u003C\/td\u003E\n\u003Ctd\u003E50-80\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50-55\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOptimal Temperature\u003C\/td\u003E\n\u003Ctd\u003E60\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMaximum cDNA Elongation\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMinimum Sample Input\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EReaction Time\u003C\/td\u003E\n\u003Ctd\u003E10 min\u003C\/td\u003E\n\u003Ctd\u003E15 min\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EInhibitor\/Contamination Resistance\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETolerance to Complex Templates\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESee for yourself why your fellow RT-PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1400px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-variations\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-Variations-to-the-system_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EVariations to the System\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/div\u003E","meta_title":"Reverse Transcription \u0026 RT-PCR","meta_keywords":"Reverse Transcription \u0026 RT-PCR","meta_description":"Reverse Transcription \u0026 RT-PCR","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":103,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":16608,"cat_no_base":"G236","parent_id":714,"description":"\u003Cp\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus cDNA Synthesis Kit\u003C\/strong\u003E contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50\u0026deg;C - 55\u0026deg;C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus is formulated with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s RNaseOFF Ribonuclease Inhibitor, offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (\u003C 1 mM), making it the best choice for ultimate RNA protection.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EMaximal flexibility in priming \u0026ndash; oligo(dT), random primers, or gene-specific primers\u003C\/li\u003E\n\u003Cli\u003ERobust cDNA synthesis from any RNA template\u003C\/li\u003E\n\u003Cli\u003EHigh reproducibility and excellent yield\u003C\/li\u003E\n\u003Cli\u003EEmploys \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (\u003Ca class=\u0022orange-link\u0022 href=\u0022http:\/\/www.abmgood.com\/PCR-Reverse-Transcriptase-(PCR)-G177.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G237\u003C\/a\u003E)\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp style=\u0022margin: 30px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E \u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E5X RT Buffer\u003C\/td\u003E\n\u003Ctd\u003E400 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOligo(dT) (10 \u0026mu;M)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ERandom Primers (10 \u0026mu;M)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EdNTP (10 mM)\u003C\/td\u003E\n\u003Ctd\u003E100 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ENuclease-Free H\u003Csub\u003E2\u003C\/sub\u003EO\u003C\/td\u003E\n\u003Ctd\u003E2 x 1.0 ml\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/td\u003E\n\u003Ctd\u003E100 rxn (100 \u0026micro;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%; margin-bottom: 20px;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022vertical-align: middle;\u0022\u003E\n\u003Ctd style=\u0022text-align: left; vertical-align: middle;\u0022 width=\u002215%\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\u0022 alt=\u0022ISO 13485:2016 MDSAP Certified\u0022 width=\u002285%\u0022 \/\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003EISO 13485:2016 MDSAP Certified\u003C\/strong\u003E\u003Cbr \/\u003EOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E","disclaimer":null,"application":"\u003Cul\u003E\n\u003Cli\u003ESynthesizing cDNA from ssRNA\u003C\/li\u003E\n\u003Cli\u003EDNA primer extension\u003C\/li\u003E\n\u003Cli\u003ESequencing dsDNA\u003C\/li\u003E\n\u003Cli\u003EConstructing cDNA library\u003C\/li\u003E\n\u003Cli\u003EProducing template for use in RT-PCR\u003C\/li\u003E\n\u003Cli\u003ELabelling 3\u0027-end of duplex DNA via end-filling reactions\u003C\/li\u003E\n\u003Cli\u003EGenerating probes for hybridization\u003C\/li\u003E\n\u003C\/ul\u003E","components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"\/g\/2\/g237-onescript-plus-reverse-transcriptase.png","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp\u003EStore at -20\u0026deg;C.\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":null,"internal_supplier":null,"internal_note":null,"inventory_location":null,"note":null,"recommend":"\u003Cstyle\u003E\n.orange-button {\nbackground-color: #e96225; \/* orange *\/\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\nwidth: 100%;\nline-height: 1em;\n}\n\n.blue-button {\nbackground-color: #1b7cef;\nborder: none;\nborder-radius: 8px;\ncolor: white;\npadding: 15px;\ntext-align: center;\ntext-decoration: none;\ndisplay: inline-block;\nfont-size: 18px;\nmargin: 4px 2px;\ncursor: pointer;\n}\n\n.table-style{\ntext-align: left;\nborder-bottom: 2px solid #f4f4f4;\n}\n\n.left{\nfloat: left;\nwidth: 48%;\n}\n\n.col-3 {\nfloat: left;\nwidth: 33%;\n}\n\n.col-3-right{\nfloat: right;\nwidth: 32%;\n}\n\n.right{\nfloat: right;\nwidth: 48%;\n}\n\n.clear{\nclear: both;\n}\n.spacing{\npadding-right: 2.5em;\n}\n\n.orange-border {\n border-radius: 0.75em;\n border: 4px solid #E96225;\n padding: 0.75em 2em 0.75em;\n}\n\n\n@media only screen and (max-width: 700px) {\n .left{\n width: 100%;\n }\n\n.right{\nwidth: 100%;\n}\n\n\n.col-3 {\nwidth: 100%;\n}\n\n.mobile{\nwidth: 100%;\n}\n\n.spacing{\npadding-right: 0;\n}\n}\n\u003C\/style\u003E\n\u003Cdiv class=\u0022left\u0022\u003E\n\u003Cp\u003E\u003Ca href=\u0022\/pcr-buffet-program\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/tinymce\/pcr-buffet-product-page-banner.png\u0022 alt=\u0022PCR buffet\u0022 width=\u0022100%\u0022 height=\u0022auto\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022right\u0022\u003E\u003C\/div\u003E","depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-09 06:40:56","updated_at":"2025-03-27 20:40:53","short_description":"\u003Cp\u003E(Equivalent to Cat. No. G233, G234, G235, G491) OneScript\u003Csup\u003E\u00ae\u003C\/sup\u003E Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse\u00a0Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal\u00a0stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb,\u00a0and is able to perform under high temperatures (50\u00b0C - 55\u00b0C), facilitating the elimination of\u00a0secondary structures associated with GC-rich RNA templates.\u00a0OneScript\u003Csup\u003E\u00ae\u003C\/sup\u003E\u00a0Plus is formulated with\u00a0\u003Cb\u003Eabm\u003C\/b\u003E\u2019s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (\u0026lt; 1 mM), making it the best choice for ultimate RNA protection.\u003C\/p\u003E","reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379585,"parent_id":714,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/g\/2\/g237-onescript-plus-reverse-transcriptase.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":0,"status":0,"entity_id_m2":18084937,"sku_in_m2":"G236","value_id_m2":49911792,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"},{"id":379586,"parent_id":714,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/g\/2\/g237-onescript-plus-stability-test_data.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18084937,"sku_in_m2":"G236","value_id_m2":49912312,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"},{"id":379587,"parent_id":714,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/r\/e\/reverse-transcriptase-selection-guide.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":18084937,"sku_in_m2":"G236","value_id_m2":49911791,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:34:05"}],"gene":null}