3rd Gen. Packaging Mix & DNAfectin™ Plus Bundle

Cat. No.

LV053-G2500

Unit

Bundle

Price

$550.00 $510.00

Cat. No.

LV053-G2500

Name

3rd Gen. Packaging Mix & DNAfectin™ Plus Bundle

Unit

Bundle

Description

abm's Third Generation lentivirus packaging plasmid mix bundled with our DNAfectin™ Plus Transfection Reagent enables a complete solution for high safety, high-efficiency lentivirus production.

1. The 3rd Generation Packaging Mix (Cat. No. LV053) consists of a mixture of three plasmids that enable viral particle production:
i) Packaging Plasmid containing structural proteins Gag, Pol.
ii) Packaging Plasmid containing structural protein Rev.
iii) Envelope Plasmid containing transmembrane glycoprotein VSV-G.

Product Mix Component	Vector Map
pLenti-P3A	Click to view
pLenti-P3B	Click to view
pLenti-P3C	Click to view

2. DNAfectin™ Plus Transfection Reagent (Cat. No. G2500) is a unique formulation of polycations and liposomes, which guarantees high transfection efficiency in a wide variety of cell types including primary cells.

Applications

Storage Condition

Refer to the storage conditions of each individual component included in the bundle.

Promotions

Bundle up, save more! These cost-saving bundles combine our best-selling Lentivirus Packaging Mix with reagents for transfection, virus titration and transduction!

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV053-G2500

Datasheet

Search CoA here

MSDS

G2500 MSDS

Supporting Protocol

Is 2nd Generation or 3rd Generation packaging mix better for lentivirus production?

The 2^nd Generation Packaging System Mix is recommended unless your facility requires 3^rd Generation Packaging Systems for stricter safety regulations.

Feature	2^nd Generation Packaging System Mix	3^rd Generation Packaging System Mix
Transfer Plasmid	Can package 2^nd or 3^rd Generation transfer plasmids (Tat dependent or independent)	Can ONLY package 3^rd Generation transfer plasmids (Tat independent)
Packaging Plasmids	One plasmid encoding Gag, Pol, Rev, and Tat	Two plasmids: one encoding Gag and Pol, and one encoding Rev
Envelope Plasmid	Interchangeable: usually encodes for VSV-G	Interchangeable: usually encodes for VSV-G
Safety	Safe. Replication incompetent: Uses 3 separate plasmids encoding various HIV genes.	Safer. Replication incompetent and always self inactivating: Uses 4 plasmids instead of 3 and eliminates the requirement for Tat.
Recombinant Virus Yield	Higher yields than 3^rd Generation due to fewer plasmids (3) required to be transfected	Lower yields than 2^nd Generation due to more plasmids (4) required to be transfected

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Controls and Related Product:

3rd Generation Packaging Mix

Cat.No. LV053

DNAfectin™ Plus Transfection Reagent

Cat.No. G2500