Watch Human Cardiomyocytes Beat
Representative human cardiomyocyte culture showing spontaneous contractions after plating.
Ready-to-use cardiac cells for time-sensitive workflows
Use T5004 when your study requires a human-relevant cardiac model without establishing an in-house differentiation workflow first.
Why Choose Embryonic Stem Cell-Derived Cardiomyocytes?
Consistent Cellular Identity
Derived from human embryonic stem cells for consistent cellular identity and reduced variability.
Beating Within 3 - 5 days
Spontaneous beating is observed within 3 - 5 days after plating.
Validated Markers
Characterized using RT-PCR and immunocytochemistry for key cardiac contractility markers.
Controlled Differentiation
Manufactured under controlled differentiation conditions to support reproducible experimental outcomes.
10–14 Day Stability
Maintains stable cardiac function for 10–14 days in culture.
Applications
Drug Discovery
Evaluate efficacy and cardiac responses in preclinical development workflows.
Cardiotoxicity Screening
Identify adverse cardiac effects earlier during compound evaluation.
Disease Modelling
Study cardiac physiology and disease mechanisms using human-relevant cell models.
Electrophysiology & Contractility Assays
Assess beating behaviour, electrical activity, and functional responses.
Tissue Engineering & Organoid Development
Build advanced 3D cardiac models and organoid systems.
Validation Data
Verified cardiac identity and function. Human Cardiomyocytes (T5004) undergo molecular, phenotypic, and functional characterization to support cardiac lineage commitment and performance.
Click images to enlarge.
Expression Analysis
Expression analysis confirms high expression of cardiac-specific markers associated with mature cardiomyocyte identity and reduced markers of stemness.
Contractile Marker Expression
Immunostaining demonstrates robust expression of key cardiac proteins, including:
- Cardiac Troponin I (cTnI) – red
- α-Actinin – green
- DAPI – blue
Functional Ion Channel Activity and Action Potential Response
Patch-clamp electrophysiology data support the functional identity of hESC-derived cardiomyocytes, showing voltage-gated sodium and potassium channel activity and action potential responses.
Sodium and Potassium Channel Activity
Voltage-gated sodium and potassium channel currents were observed from a hESC-cardiomyocyte using a step depolarization protocol.
Ramp Depolarization Response
Action potential response was induced by ramp depolarization from -80 mV to 0 mV in voltage-clamp mode.
Representative electrophysiology traces: data shown are from hESC-derived cardiomyocytes and support functional cardiac behaviour alongside marker validation and beating activity.
TM213 Differentiation Medium Kit
Generate Functional Cardiomyocytes In-House
For researchers who prefer complete control over differentiation workflows, the TM213 Differentiation Medium Kit provides a streamlined solution for generating functional cardiomyocytes directly from their own human embryonic stem cells.
Using optimized differentiation protocols and validated reagents, researchers can establish their own cardiomyocyte production workflow while leveraging the same expertise used to create T5004.
Why Use the TM213 Differentiation Medium Kit?
Run the Workflow In-House
Perform differentiation within your own laboratory environment.
Project-Based Production
Generate cardiomyocytes based on project demands.
Optimized Workflow
Optimized workflow designed to support consistent differentiation outcomes.
Downstream Integration
Integrates seamlessly with downstream applications including toxicity testing, disease modelling, and 3D culture systems.
Promotional Cardiac Research Bundle
Get the core products needed to build a cardiomyocyte culture workflow with ready-to-use cells, extracellular matrix support, and maintenance medium.
T5004 + 3DCelMatrix™ + Maintenance Medium Kit
Promotional bundle includes Human Cardiomyocytes, 3DCelMatrix™, and Human Cardiomyocyte Maintenance Medium Kit.
Frequently Asked Questions
Common questions about T5004 Human Cardiomyocytes, validation, functional performance, and in-house differentiation options.
How quickly do T5004 cardiomyocytes begin beating?
What validation is performed?
How long are the cells functionally stable?
What electrophysiology data are available?
Can I generate my own cardiomyocytes instead?