Lentivirus siRNA Library
abm's RNAi Expression System is available in lentivirus format allowing for efficient siRNA expression through transfection or infection of target cells. Featuring a unique convergent promoter design, it enhances gene knockdown without requiring a hairpin loop structure. abm offers a comprehensive library of human, mouse, and rat siRNAs available in vector or pre-packaged lentivirus format.
Search our collection of siRNA Technology products using Gene symbol or Accession number:
Advantages of Lentivirus siRNA:
- Utilizes potent 27-29 bp oligos compared to traditional 19 or 21 bp oligos.
- Features convergent promoters to eliminate hairpin loop structures, simplifying sequencing and plasmid propagation.
- Available GFP reporter for real time monitoring transfection or viral infection.
- Available as a set of 4 constructs for enhanced gene knockdown.
Additional Information
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Workflow
Click to view. -
Vector Map
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Lenti-siRNA Expression Systems Manual
Click to download.
Top Publications
Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling.
Bruno NE. et al.
Nature Chemical Biology (2021)
doi: 10.1038/s41589-020-00719-w
HOTAIR interacts with PRC2 complex regulating the regional preadipocyte transcriptome and human fat distribution.
Kuo FC. et al.
Cell Reports (2022)
doi: 10.1016/j.celrep.2022.111136
ROS-Mediated Apoptotic Cell Death of Human Colon Cancer LoVo Cells by Milk δ-Valerobetaine.
D'Onofrio N. et al.
Scientific Reports (2020)
doi: 10.1038/s41598-020-65865-6
FAQs
| Is the siRNA set composed of lentiviral vectors with shRNAs or siRNAs? Do you validate the RNA interference? |
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Our RNA interference lentiviral vectors contain siRNAs. We employ a dual convergent promoter system where the sense and antisense strands of the siRNA are expressed by two different promoters rather than in a hairpin loop - to avoid any possible recombination events that can occur.
In cases where there are no verified and published siRNA sequences for your gene of interest, we use our siRNA design software to locate suitable target sites. If these designed siRNAs don't give efficient knock down of gene expression in your experiments, we offer a onetime replacement (free of charge) that will contain a new set of sequences to try. abm guarantees that at least one out of the four siRNA Lentivector constructs purchased in a set will give over 70% knockdown efficiency within appropriate target cells showing >80% transfection efficiency. If these four constructs are still considered to be ineffective, then it is most likely the gene is not susceptible to siRNA knockdown. |