Column-Pure Plasmid Miniprep Kit
Cat. No.
|
G4003
Print
|
Name
|
Column-Pure Plasmid Miniprep Kit
|
Unit
|
250 Preparations
|
Description
|
abm’s Column-Pure Plasmid Miniprep Kit utilizes the standard alkaline lysis method with silica spin column technology to rapidly extract and purify plasmid DNA from cells. The extracted high quality plasmid DNA is primarily in supercoiled form and is ready for downstream applications such as PCR, restriction digest and sequencing.
Principle: The procedure involves an alkaline lysis method to break down bacterial cells, followed by DNA adsorption onto silica in the presence of high salt. The unique silica membrane replaces traditional glass or silica slurries, streamlining the plasmid DNA preparation. The process includes three steps: 1) bacterial lysate preparation and clearing via alkaline lysis, 2) transfer of the supernatant to a column for DNA binding, and 3) washing away proteins and impurities, followed by nucleic acid elution with a low-salt buffer.
Key Features:
- Value-priced
- 15 min simplified protocol
- High Capacity - up to 35μg of DNA per column
- Extracts small & large sized plasmids
- No phenol chloroform or ethanol precipitation required
|
Storage Condition
|
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37°C to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2-8°C.
|
Material Citation
|
If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G4003
|
There are no references for this product yet!
This product has no review yet.
Controls and Related Product:
{"id":3424,"cat_no":"G4003","sku_in_m2":"","old_cat_no":"","name":"Column-Pure Plasmid Miniprep Kit","category_id":59,"category_name":"DNA \u0026 RNA Purification Kits","frontend_category_id":14,"frontend_category_ids":"1,14","gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"250 Preparations","accession_number":"","species_id":0,"species":"","raised_in_id":0,"raised_in":"","cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","price":"215.0000","price_before_discount":"0.0000","keywords":"","invisible_search_keywords":"","filter_group_id":34,"filter_group_name":"Molecular Biology","filter_id":39,"filter_name":"DNA \u0026 RNA Purification Kits","search_filter_ids":null,"search_filter_keys":null,"temp_field":null,"customization":0,"filter_table_id":0,"filter_table_name":"","related_group_id":null,"url":"","operator":"","points_rule":1,"status":1,"need_remove":0,"document_sort":"","price_old":null,"price2024":null,"created_at":"2025-03-06 19:28:49","updated_at":"2025-03-26 23:07:11","Confirmed":"Need Confirm","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"DNA-RNA-Purification-Kits.html","frontendBreadcrumbs":[{"id":1,"pid":86,"name":"General Materials","page_id":27,"page_type":"App\\Models\\Category","href":null,"sort":1,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"general-materials.html","url":"general-materials.html"},{"id":14,"pid":1,"name":"DNA\/RNA Purification","page_id":59,"page_type":"App\\Models\\Category","href":null,"sort":14,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"DNA-RNA-Purification-Kits.html","url":"DNA-RNA-Purification-Kits.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseMolecular","geo_price":"215.00","geo_price_symbol":"$215.00","category":{"id":59,"categories":"DNA \u0026 RNA Purification Kits","pid":27,"url_key":"DNA-RNA-Purification-Kits.html","css":null,"wid":0,"breadcrumbs":"[{\u0022url\u0022:\u0022https:\/www.abmgood.com\/\u0022,\u0022title\u0022:\u0022Home\u0022},{\u0022url\u0022:\u0022https:\/www.abmgood.com\/general-materials.html\u0022,\u0022title\u0022:\u0022General Materials \u0026amp; Others\u0022},{\u0022url\u0022:\u0022https:\/\/www.abmgood.com\/DNA-RNA-Purification-Kits.html\u0022,\u0022title\u0022:\u0022DNA \u0026amp; RNA Purification Kits\u0022}]","breadcrumbsIdJson":"\u003Cscript type=\u0022application\/ld+json\u0022\u003E{\u0022@context\u0022:\u0022https:\/\/schema.org\u0022,\u0022@type\u0022:\u0022BreadcrumbList\u0022,\u0022itemListElement\u0022:[{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:1,\u0022name\u0022:\u0022Home\u0022,\u0022item\u0022:\u0022https:\/www.abmgood.com\/\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:2,\u0022name\u0022:\u0022General Materials \u0026amp; Others\u0022,\u0022item\u0022:\u0022https:\/www.abmgood.com\/general-materials.html\u0022},{\u0022@type\u0022:\u0022ListItem\u0022,\u0022position\u0022:3,\u0022name\u0022:\u0022DNA \u0026amp; RNA Purification Kits\u0022}]}\u003C\/script\u003E","breadcrumbsHtml":"\u003Cul class=\u0022abm-breadcrumb\u0022\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/www.abmgood.com\/\u0022\u003EHome\u003C\/a\u003E\u003C\/li\u003E\u003Cli\u003E\u003Ca href=\u0022https:\/www.abmgood.com\/general-materials.html\u0022\u003EGeneral Materials \u0026amp; Others\u003C\/a\u003E\u003C\/li\u003E\u003Cli class=\u0022active\u0022\u003EDNA \u0026amp; RNA Purification Kits\u003C\/li\u003E\u003C\/ul\u003E","description":"\u003Ch2 class=\u0022abm-categories-title-h2\u0022\u003EDNA \u0026amp; RNA Purification Kits\u003C\/h2\u003E\n\u003Cul class=\u0022abm-page-category-nav-list\u0022 style=\u0022margin-top: -20px;\u0022\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#genomic-dna-extraction-kits\u0022 class=\u0022abm-page-category-nav-link\u0022\u003EGenomic DNA Extraction Kits\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#dna-extraction-purification-kits\u0022 class=\u0022abm-page-category-nav-link\u0022\u003EDNA Extraction and Purification Kits\u003C\/a\u003E\u003C\/li\u003E\n\u003Cli class=\u0022abm-page-category-nav-item\u0022\u003E\u003Ca href=\u0022#rna-extraction-purification-kits\u0022 class=\u0022abm-page-category-nav-link\u0022\u003ERNA Extraction and Purification Kits\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-md-12\u0022 style=\u0022text-align: left; padding-bottom: 3em;\u0022\u003E\n\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a variety of specialty DNA and RNA kits allowing for efficient extraction from various sources such as animal, plant, blood, bacteria and fungi. Additionally you can find affordable and reliable kits for routine techniques such as Gel \u0026amp; PCR clean up purification, as well as specialized techniques such as circRNA isolation.\u0026nbsp;\u003C\/p\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module\u0022 style=\u0022width: 100%;\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable id=\u0022table2\u0022 class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022width: 100%;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left; width: 49.6%;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth style=\u0022width: 11.225%;\u0022\u003ECat. No.\u003C\/th\u003E\n\u003Cth style=\u0022width: 12.375%;\u0022\u003EUnit\u003C\/th\u003E\n\u003Cth style=\u0022width: 12.9%;\u0022\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr align=\u0022left\u0022 style=\u0022background-color: #f8f9fa;\u0022 id=\u0022genomic-dna-extraction-kits\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left; width: 86.1%;\u0022\u003E\u003Cstrong\u003EDNA Extraction and Purification Kits\u003C\/strong\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca href=\u0022\/Column-Pure-Plasmid-Miniprep-Kit-G4003.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Plasmid Miniprep Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/Column-Pure-Plasmid-Miniprep-Kit-G4003.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG4003\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E250 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G4003\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-gel-and-pcr-clean-up-kit.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Gel and PCR Clean-Up Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-gel-and-pcr-clean-up-kit.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED516\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E100 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022D516\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca href=\u0022\/dna-purification-magnetic-beads-5-ml-g950-vin.html\u0022 style=\u0022color: #ef6331;\u0022\u003EDNA Purification Magnetic Beads\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/dna-purification-magnetic-beads-5-ml-g950-vin.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG950\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E5 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G950\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca href=\u0022\/dna-purification-magnetic-beads-25-ml.html\u0022 style=\u0022color: #ef6331;\u0022\u003EDNA Purification Magnetic Beads\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/dna-purification-magnetic-beads-25-ml.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG951\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E25 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G951\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr align=\u0022left\u0022 style=\u0022background-color: #f8f9fa;\u0022 id=\u0022dna-extraction-purification-kits\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left; width: 86.1%;\u0022\u003E\u003Cstrong\u003ERNA Extraction and Purification Kits\u003C\/strong\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/Column-Pure-RNA-Miniprep-Kit-G4002.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure RNA Miniprep Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/Column-Pure-RNA-Miniprep-Kit-G4002.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG4002\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G4002\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-fungi-rna-isolation-kit-d510.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Fungi RNA Isolation Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-fungi-rna-isolation-kit-d510.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED510\u003C\/a\u003E\u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022\/Column-Pure-Plasmid-Miniprep-Kit-G4003.html\u0022 style=\u0022color: #ef6331;\u0022\u003E\u003C\/a\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022D510\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca href=\u0022RNA-purification-magnetic-beads-G971.html\u0022 style=\u0022color: #ef6331;\u0022\u003ERNA Purification Magnetic Beads\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022RNA-purification-magnetic-beads-G971.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG971\u003C\/a\u003E\u003Ca href=\u0022\/dna-purification-magnetic-beads-5-ml-g950-vin.html\u0022 style=\u0022color: #ef6331;\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E5 ml\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G971\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca href=\u0022\/circRNA-isolation-kit-G4001.html\u0022 style=\u0022color: #ef6331;\u0022\u003EcircRNA Isolation Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/circRNA-isolation-kit-G4001.html\u0022 style=\u0022color: #ef6331;\u0022\u003EG4001\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E25 Reactions\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G4001\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr align=\u0022left\u0022 style=\u0022background-color: #f8f9fa;\u0022 id=\u0022rna-extraction-purification-kits\u0022\u003E\n\u003Ctd colspan=\u00224\u0022 style=\u0022text-align: left; width: 86.1%;\u0022\u003E\u003Cstrong\u003EGenomic DNA Extraction Kits\u003C\/strong\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-blood-genomic-dna-kit-d203.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Blood Genomic DNA Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-blood-genomic-dna-kit-d203.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED203\u003C\/a\u003E\u003Ca href=\u0022\/Column-Pure-RNA-Miniprep-Kit-G4002.html\u0022 style=\u0022color: #ef6331;\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G4002\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-bacterial-genomic-dna-isolation-kit-d511.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Bacterial Genomic DNA Isolation Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-bacterial-genomic-dna-isolation-kit-d511.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED511\u003C\/a\u003E\u003Ca href=\u0022\/column-pure-fungi-rna-isolation-kit-d510.html\u0022 style=\u0022color: #ef6331;\u0022\u003E\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022D511\u0022 style=\u0022width: 12.9%;\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-animal-genomic-dna-isolation-kit-d512.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Animal Genomic DNA Isolation Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-animal-genomic-dna-isolation-kit-d512.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED512\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.9%;\u0022 class=\u0022product-price\u0022 data-product=\u0022d512\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 49.6%;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/column-pure-plant-dna-extraction-kit.html\u0022 style=\u0022color: #ef6331;\u0022\u003EColumn-Pure Plant DNA Extraction Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 11.225%;\u0022\u003E\u003Ca href=\u0022\/column-pure-plant-dna-extraction-kit.html\u0022 style=\u0022color: #ef6331;\u0022\u003ED519\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.375%;\u0022\u003E50 Preps\u003C\/td\u003E\n\u003Ctd style=\u0022width: 12.9%;\u0022 class=\u0022product-price\u0022 data-product=\u0022d519\u0022\u003E$0\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022module abm-category-module col-md-12\u0022\u003E\n\u003Cdiv class=\u0022col-sm-4 col-md-4\u0022\u003E\n\u003Cp\u003E\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"DNA \u0026 RNA Purification Kits","meta_keywords":"DNA purification kit,DNA gel extraction kit,RNA extraction kit,RNA purification kit,miniprep kit","meta_description":"abm\u00a0offers a variety of affordable, rapid and reliable DNA and RNA kits allowing for efficient extraction or purification from various sources, including animal, plant, blood, bacteria and fungi.","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":112,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":18602,"cat_no_base":null,"parent_id":3424,"description":"\u003Cp data-start=\u0022111\u0022 data-end=\u0022593\u0022\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s \u003Cstrong\u003EColumn-Pure Plasmid Miniprep Kit\u003C\/strong\u003E utilizes the standard alkaline lysis method with silica spin column technology to rapidly extract and purify plasmid DNA from cells. The extracted high quality plasmid DNA is primarily in supercoiled form and is ready for downstream applications such as PCR, restriction digest and sequencing.\u003C\/p\u003E\n\u003Cp data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003EPrinciple:\u003C\/strong\u003E The procedure involves an alkaline lysis method to break down bacterial cells, followed by DNA adsorption onto silica in the presence of high salt. The unique silica membrane replaces traditional glass or silica slurries, streamlining the plasmid DNA preparation. The process includes three steps: 1) bacterial lysate preparation and clearing via alkaline lysis, 2) transfer of the supernatant to a column for DNA binding, and 3) washing away proteins and impurities, followed by nucleic acid elution with a low-salt buffer.\u003C\/p\u003E\n\u003Cp data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong data-start=\u0022595\u0022 data-end=\u0022609\u0022\u003EKey Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003EValue-priced\u003C\/strong\u003E\u003C\/li\u003E\n\u003Cli data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003E15 min simplified protocol\u003C\/strong\u003E\u003C\/li\u003E\n\u003Cli data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003EHigh Capacity - up to 35\u0026mu;g of DNA per column\u003C\/strong\u003E\u003C\/li\u003E\n\u003Cli data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003EExtracts small \u0026 large sized plasmids\u003C\/strong\u003E\u003C\/li\u003E\n\u003Cli data-start=\u0022595\u0022 data-end=\u00221183\u0022\u003E\u003Cstrong\u003ENo phenol chloroform or ethanol precipitation required\u003C\/strong\u003E\u003C\/li\u003E\n\u003C\/ul\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":"\u003Cp\u003EThe kit components can be stored dry at room temperature (15-25\u0026deg;\u003Cspan lang=\u0022EN-US\u0022\u003EC) and are stable for at least 18 months under these conditions. \u003C\/span\u003E\u003Cspan lang=\u0022EN-US\u0022\u003EIf any precipitates form in the buffers, warm at 37\u003C\/span\u003E\u0026deg;C\u003Cspan lang=\u0022EN-US\u0022\u003E\u0026nbsp;to dissolve. After addition of RNase A\u003C\/span\u003E\uff0c\u003Cspan lang=\u0022EN-US\u0022\u003EBuffer P1 is stable for 6 months when stored at 2-8\u0026deg;C.\u003C\/span\u003E\u003C\/p\u003E","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":"P100103","stain_color":null,"supplier":"Magen","internal_supplier":null,"internal_note":null,"inventory_location":null,"note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2025-03-06 19:28:49","updated_at":"2025-03-26 23:07:11","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"media":[{"id":388549,"parent_id":3424,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/UzNtgS2GOEB561iT3TwAAT6imH3nWPQMVRzXlYOC.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-10 20:59:09","updated_at":"2025-03-10 20:59:14"}],"gene":null}