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StemRelease™ Dissociation Reagent

Cat. No.

TM097

Unit

100 ml

Price

$158.00

Cat. No.	TM097
Name	StemRelease™ Dissociation Reagent
Description	StemRelease™ Dissociation Reagent is a gentle, enzyme-free cell dissociation reagent designed for routine passaging and harvesting of human pluripotent stem cells (hPSCs), including human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). It enables efficient detachment of hPSC colonies while maintaining cells as multicellular aggregates, helping preserve cell viability, colony-forming ability, and pluripotency. StemRelease™ Dissociation Reagent is suitable for routine hPSC passaging, expansion, cryopreservation preparation, and downstream differentiation applications. Key Features Gentle, enzyme-free dissociation of hPSC colonies Maintains cells as multicellular aggregates rather than single cells Suitable for routine passaging, expansion, and cryopreservation workflows Ready-to-use formulation with no dilution required Compatible with commonly used hPSC culture matrices and maintenance media StemRelease™ Dissociation Reagent is compatible with commonly used feeder-free hPSC culture systems, including: Vitronectin-coated culture systems Matrix-based coating systems including Matrigel and 3DCelMatrix™ Commonly used hPSC maintenance media
Unit	100 ml
Usage	Recommended Companion Products 3DCelMatrix™ (TM076) - Matrix coating system ROCK Inhibitor Solution (1000X) (TM172) - optional support following passaging, thawing, or other stressful handling conditions, as required by the specific culture protocol CryoGuard™ Freezing Media (TM078) - cryopreservation medium Recommended Workflow Wash cells once with 1X DPBS, No Ca²⁺, No Mg²⁺ (CH110). Add sufficient StemRelease™ Dissociation Reagent to cover the cell surface, approximately 2 ml/well for a 6-well plate or 4 ml for a 10 cm dish. Incubate at room temperature for approximately 4 minutes, or until colonies begin to shrink and show visible separation at the colony edges. Carefully aspirate StemRelease™ without disturbing the colonies. Leave the cells at room temperature for an additional 1–3 minutes to allow the colonies to loosen further. Add hPSC maintenance medium and gently pipette to detach and resuspend the colonies as small multicellular aggregates. Avoid excessive pipetting or single-cell dissociation. Transfer the cell aggregates to freshly coated culture vessels for passaging or proceed with cryopreservation as required.
Appearance	Liquid
Application	Routine maintenance and passaging of human iPSCs and ESCs Generation of small multicellular aggregates for hPSC expansion Preparation of aggregates for embryoid body (EB) formation Stem cell banking and cryopreservation workflows Feeder-free pluripotent stem cell culture systems
Shipping Conditions	Ice Pack
Storage Condition	Store at −20°C. After thawing, store at 2–8°C and avoid repeated freeze-thaw cycles.
Disclaimer	abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM097

Datasheet

TM097 Datasheet

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MSDS

TM097 MSDS

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Controls and Related Product:

3DCelMatrix™

Cat.No. TM076

ROCK Inhibitor Solution (1000X)

Cat.No. TM172

CryoGuard™ Freezing Media

Cat.No. TM078

1X DPBS, No Ca²⁺, No Mg²⁺

Cat.No. CH110

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Applied Biological Materials Inc. (abm) #1-3671 Viking Way, Richmond, BC, V6V 2J5, Canada
info@abmgood.com Local: 604-247-2416 Toll Free: 1-866-757-2414

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