StemRelease™

Cat. No.
TM097
Unit
100 ml
Price
$158.00
Cat. No. TM097
Name StemRelease™
Description

StemRelease™ is a gentle, enzyme-free cell dissociation reagent designed for the selective detachment and routine passaging of human pluripotent stem cells (hPSCs), including human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs).

This ready-to-use reagent enables rapid colony release while preserving multicellular aggregate integrity, cell viability, and pluripotency. StemRelease™ supports reproducible feeder-free stem cell culture workflows and is optimized for routine maintenance, colony selection, cryopreservation preparation, and downstream differentiation applications.

StemRelease™ is designed to minimize cellular stress associated with enzymatic dissociation and supports stable long-term expansion of undifferentiated hPSC cultures.

 

Key Features

  • Gentle enzyme-free dissociation workflow
  • Selective detachment of undifferentiated hPSC colonies
  • Maintains multicellular aggregates during routine passaging
  • Supports high post-passage viability and pluripotency maintenance
  • Ready-to-use formulation with no dilution required
  • Compatible with feeder-free culture systems
  • Optimized for human iPSC and ESC maintenance workflows
  • Suitable for routine culture, colony selection, banking, and differentiation preparation

 

StemRelease™ has been validated in feeder-free human pluripotent stem cell workflows using:

  • Vitronectin-coated culture systems
  • Matrix-based coating systems including Matrigel and 3DCelMatrix™
  • Defined and protein-containing stem cell maintenance media
Unit 100 ml
Usage

Recommended Companion Products

  • 3DCelMatrix™ (TM076) — Matrix coating system
  • ROCK Inhibitor Y-27632 (TM131)
  • CryoGuard™ Freezing Media (TM078)

 

Recommended Workflow

  1. Wash cells once with1X DPBS, No Ca²⁺, No Mg²⁺ (CH110).
  2. Add StemRelease™ to culture vessels and incubate briefly.
  3. Aspirate reagent and incubate the plate until colony edges begin to loosen.
  4. Add maintenance medium and gently detach colonies as small multicellular aggregates.
  5. Passage cells onto freshly coated culture vessels.

For optimal recovery, use ROCK Inhibitor Y-27632 (TM131) during stressful handling conditions such as thawing or single-cell applications.
Appearance Liquid
Application
  • Routine maintenance and passaging of human iPSCs and ESCs
  • Selective removal and enrichment of undifferentiated colonies
  • Preparation of aggregates for embryoid body (EB) formation
  • Stem cell banking and cryopreservation workflows
  • Feeder-free pluripotent stem cell culture systems
Storage Condition Store at room temperature, away from light. Do not freeze. Shelf Life After Opening: 6 months.
Disclaimer
  1. abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.
  2. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM097
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