BlasTaq™ HotStart DNA Polymerase

Cat. No.
G595
Unit
400 rxn
Price
$129.00

PCR buffet

Cat. No. G595
Name BlasTaq™ HotStart DNA Polymerase
Unit 400 rxn
Category PCR Polymerase
Description

Eliminate non-specific amplification and primer-dimer formation!

BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.

Product Features:

  • No non-specific amplification or primer-dimers
  • Improves yield of desired product
  • Simplifies reaction setup
  • Available as a MasterMix (Cat. No. G598)
  • Included in abm's PCR Buffet Program

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Product Component Quantity
BlasTaq™ HotStart DNA Polymerase 400 rxn (200 µl)
5X BlasTaq™ Buffer 1 2 x 1.0 ml

 1 Buffer contains 1.5 mM Mg2+.

ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Storage Condition

Store at -20°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G595
Datasheet
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MSDS
  • Zahid, W., Farooqui, N., Zahid, N., Ahmed, K., Anwar, M. F., Rizwan-ul-Hasan, S., … Abidi, S. H. (2023). Association of Interferon Lambda 3 and 4 Gene SNPs and Their Expression with COVID-19 Disease Severity: A Cross-Sectional Study. Infection and Drug Resistance, 16, 6619–6628. https://doi.org/10.2147/IDR.S422095

    Song, Z., Hu, K., Rao, J., Cheng, B., Xu, L., An, R., & Liang, X. (2023). Unexpected Mechanism and Inhibition Effect for Nonspecific Amplification Involving Dynamic Binding of Primers with Background DNA. Analytical Chemistry, 95(46), 16819-16829. https://doi.org/10.1021/acs.analchem.3c02274

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