CRISPR Scrambled sgRNA Lentivirus (for spCas9)
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Product Name
CRISPR Scrambled sgRNA Lentivirus (for spCas9) -
Unit
4 x 500µl -
Description
Control CRISPR Lentivirus with scrambled sgRNA for spCas9.
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Vector Map
pLenti-U6-sgRNA-PGK-Neo (click blue link to view) -
Titer
107 IU/mL -
Target Sequence
GCACTCACATCGCTACATCA -
Storage Condition
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
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Disclaimer
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Caution
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Print/Download Datasheet
Dai, X., Chen, X., Hakizimana, O., & Mei, Y. "Genetic interactions between ANLN and KDR are prognostic for breast cancer survival" Oncology Reports. : (2019).
Dai, X., Chen, X., Hakizimana, O., & Mei, Y. (2019). Genetic interactions between ANLN and KDR are prognostic for breast cancer survival. Oncology Reports. https://doi.org/10.3892/or.2019.7332
ABM community
Verified customer
Asked on Feb 28 2025
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Feb 28 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
ABM Scientific Support
Answered on Mar 24 2025
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Current vector selected:
CRISPR Scrambled sgRNA Lentivirus (for spCas9)
Cat. No.
K019
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
Empty
×
Current vector selected:
CRISPR Scrambled sgRNA Lentivirus (for spCas9)
Cat. No.
K019
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivirus-with-neomycin-lvp015-n.html\u0022 rel=\u0022noopener\u0022\u003EScrambled siRNA GFP Lentivirus with Neomycin\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-sirna-gfp-lentivirus-with-neomycin-lvp015-n.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELVP015-N\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVirus\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003ENeo\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LVP015-N\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-aav-sirna-control-vector-iaav01500amp.html\u0022 rel=\u0022noopener\u0022\u003EScrambled AAV siRNA Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/scrambled-aav-sirna-control-vector-iaav01500amp.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003EiAAV01500\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVector\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EGFP-2A-Puro\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022iAAV01500\u0022\u003E140.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=Scrambled+AAV+siRNA+Control+Virus+\u0022 rel=\u0022noopener\u0022\u003EScrambled AAV siRNA Control Virus\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/search?query=Scrambled+AAV+siRNA+Control+Virus+\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003EBy Serotype\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVirus\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EGFP-2A-Puro\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022\u003E$255.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022background-color: #f8f9fa;\u0022 align=\u0022left;\u0022 id=\u0022table-product-mini-category-LentivirusControls\u0022\u003E\n\u003Ctd style=\u0022text-align: left; width: 46.6159%; height: 22px;\u0022 colspan=\u00225\u0022\u003E\u003Cspan style=\u0022color: #00000;\u0022\u003E\u003Cstrong\u003ELentivirus Controls\u003C\/strong\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-iii-blank-vector-lv587.html\u0022 rel=\u0022noopener\u0022\u003ELenti-CMV-Blank-SV40-Puro Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022outline: 0px; user-select: none;\u0022 href=\u0022\/plenti-iii-blank-vector-lv587.html\u0022 rel=\u0022noopener\u0022\u003ELV587\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003EVector\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV587\u0022\u003E140.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-iii-blank-lentivirus-lvp687.html\u0022 rel=\u0022noopener\u0022\u003ELenti-III-Blank Lentivirus\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 style=\u0022outline: 0px; user-select: none;\u0022 href=\u0022\/lenti-iii-blank-lentivirus-lvp687.html\u0022 rel=\u0022noopener\u0022\u003ELVP687\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003EVirus\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LVP687\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 44px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-cmv-gfp-2a-puro-blank-vector-lv590.html\u0022 rel=\u0022noopener\u0022\u003ELenti-CMV-Blank-CBH-GFP-2A-Puro Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 44px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-cmv-gfp-2a-puro-blank-vector-lv590.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 44px;\u0022\u003EVector\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 44px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV590\u0022\u003E140.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 44px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-cmv-gfp-2a-puro-blank-lentivirus-lvp690.html\u0022 rel=\u0022noopener\u0022\u003ELenti-CMV-Blank-CBH-GFP-2A-Puro Control Lentivirus\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 44px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-cmv-gfp-2a-puro-blank-lentivirus-lvp690.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELVP690\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 44px;\u0022\u003EVirus\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 44px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LVP690\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-ef1a-blank-vector-lv588.html\u0022 rel=\u0022noopener\u0022\u003ELenti-EF1a-Blank-SV40-Puro Control Vector\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/plenti-ef1a-blank-vector-lv588.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV588\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003EVector\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003EPuro\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV588\u0022\u003E140.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-ef1a-blank-lentivirus-lvp688.html\u0022 rel=\u0022noopener\u0022\u003ELenti-EF1a-Blank Lentivirus\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/lenti-ef1a-blank-lentivirus-lvp688.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELVP688\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVirus\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EPuro\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LVP688\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-control-lentivirus-puro-lv095.html\u0022 rel=\u0022noopener\u0022\u003EGFP Control Lentivirus (Puro)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-control-lentivirus-puro-lv095.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV095\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; height: 22px;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EVirus\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%;\u0022\u003E\u003Cspan style=\u0022color: #000000;\u0022\u003EPuro\u003Cbr \/\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 7.26849%; height: 22px;\u0022 class=\u0022product-price\u0022 data-product=\u0022LV095\u0022\u003E205.00\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 22px;\u0022\u003E\n\u003Ctd style=\u0022width: 21.2345%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-control-lentivirus-neo-lv006.html\u0022 rel=\u0022noopener\u0022\u003EGFP Control Lentivirus (Neo)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 9.16127%; height: 22px;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/gfp-control-lentivirus-neo-lv006.html\u0022 style=\u0022outline: 0px; user-select: none;\u0022 rel=\u0022noopener\u0022\u003ELV006\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 4.47581%; 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