DNase I (RNase-Free)
Cat. No.
E091
Unit
200 U (100 μl)
Price
$102.00
| Cat. No. | E091 | ||||||
| Name | DNase I (RNase-Free) | ||||||
| Unit | 200 U (100 μl) | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description |
DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase-free and can be used in RNA applications.
|
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| Application |
• Removes DNA from protein preparations and RNA samples |
||||||
| Concentration | 2 U/μl | ||||||
| Note |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. Caution: Not for diagnostic use. |
||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E091 |
Datasheet
Search CoA here
| How should DNase I be stored? | |
|
DNase I should be stored at -20°C. To avoid repeated freeze-thaw cycles, it is recommended to aliquot the enzyme before freezing.
|
| Can DNase I be used for RNA purification? | |
|
Yes, DNase I (RNase-Free) is designed for RNA applications and can be used to remove genomic DNA contamination from RNA samples.
|
| How long should I incubate my sample with DNase I? | |
|
The digestion should not exceed 15 minutes to prevent RNA degradation.
|
| How do I stop the DNase I reaction? | |
|
The reaction can be stopped by:
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| How much DNase I should I use per reaction? | |
|
Typically, 1 unit of DNase I is sufficient to degrade 1 µg of DNA in 10 minutes at 37°C. Adjust enzyme concentration based on the DNA amount and reaction conditions.
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01:04:45"},"info":{"id":808,"cat_no_base":"E091","parent_id":249,"description":"\u003Cp\u003E\u003Cstrong\u003EDNase I\u003C\/strong\u003E is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single\/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and\/or oligonucleotide (5\u0026rsquo;-phosphorylated and 3\u0026rsquo;-hydroxylated) end-products. 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