Human Cardiomyocyte Differentiation Medium Kit

Cat. No.

TM213

Unit

1 Kit

Price

$699.00

Cat. No.

TM213

Name

Human Cardiomyocyte Differentiation Medium Kit

Description

The Human Cardiomyocyte Differentiation Medium Kit is a ready-to-use, defined, serum-free medium system designed to efficiently differentiate human embryonic stem cells (hESCs) into cardiomyocyte-like cells in a monolayer culture format.

The kit includes stage-specific media that support cardiac lineage induction and specification, promoting robust and reproducible differentiation. Under recommended culture conditions, spontaneously beating cardiomyocyte networks are typically observed approximately 9 days after differentiation is initiated (Day 0), following the pre-differentiation cell expansion and preparation phase. The formulation minimizes batch-to-batch variability and supports scalable, consistent differentiation workflows.

Explore the performance data and validation images here.

This kit is supplied as a basal medium and stage-specific supplement components to be combined to prepare media for stepwise differentiation:

Product Components	Part No.	Quantity	Storage Condition
Basal Medium 6	BM006-100	100 ml	2-8°C
HCDMK - Supplement 1	TM213-1	96 µl	-20°C
HCDMK - Supplement 2	TM213-2	90 µl	-20°C
HCDMK - Supplement 3	TM213-3	96 µl	-20°C
B27 Supplement (50X)	SM001-2	2 ml	-20°C
B27 Supplement (50X), without Insulin	SM007-2	2 ml	-20°C
HCDMK - Selection Medium	TM213-4	25 ml	2-8°C

Additional required materials (not included in kit)

Human embryonic stem cells (hESCs)
Stem cell growth medium (mTeSR)
1X DPBS, No Ca²⁺, No Mg²⁺ (CH110)
3DCelMatrix™ (TM076)
DMEM/F-12 Medium (TM510)

For complete usage instructions, consult the Datasheet.

Unit

1 Kit

Usage

A stage-specific cardiomyocyte differentiation system with built-in selection/enrichment for generating highly enriched, functional beating cardiomyocytes.

Sterility

Sterile

Appearance

Liquid

Application

Cardiac differentiation research – generate hESC-derived cardiomyocytes for studying cardiac development and maturation.
Drug screening & cardiotoxicity testing – assess cardiac responses and safety profiles of compounds.
Disease modeling – study cardiac disorders and therapeutic strategies using human cardiomyocytes.
Cardiac tissue engineering – support development of engineered cardiac models and 3D culture systems.

Each lot has been fully tested and optimized for its ability to support in vitro cell culture. The testing parameters include: adherence rate, typical morphology, and sterility.

Storage Condition

For individual component storage conditions, refer to the table in the description section. Complete medium can be stored at 2-8°C for up to 30 days.

Disclaimer

abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans.
abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. TM213

Datasheet