Lentivirus Promoter Blast™ kit
Cat. No.
LV950
Unit
4 x 1ml
Price
$643.00
| Cat. No. | LV950 | |||||||||||||||||||||||||||||||||
| Name | Lentivirus Promoter Blast™ kit | |||||||||||||||||||||||||||||||||
| Unit | 4 x 1ml | |||||||||||||||||||||||||||||||||
| Description |
abm's Lentivirus Promoter Blast™ Kit is designed for easy monitoring and optimization of lentivirus transduction efficiency to help you achieve successful results in your stable cell line generation or engineering experiment. This kit includes 4 different GFP Lentiviruses (4 x 1ml at >1x106 IU/ml) with either the CMV, PGK, EF1α, or UBC promoter, enabling you to determine which promoter works best for your cell line. In addition, this kit can be used to calculate the optimal Multiplicity of Infection (MOI) for your transduction experiment - simply transduce your cells at various MOIs and monitor GFP signals! Kit features:
After the optimal infection condition and promoter is identified, the lentivirus with your target gene and under your chosen promoter may be ordered separately either in low or high titer format.
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| Note |
Infection Protocol 1. Plate the target cells in a 24-well plate, 24 hours prior to viral infection at a density of 0.5×105 cells per well. Add 0.5 ml of complete optimal medium (with serum and antibiotics if required) and incubate the cells at 37°C with 5% CO2 overnight. Note: It is possible to use other plate formats for transduction. In this case, the amount of cells should be adjusted depending on the growth area of the well/plate. 2. Thaw the lentivirus from –80°C at room temperature. We strongly suggest to make usable aliquots of lentivirus to avoid free-thaw since that is known to reduce the viral titer.Calculate the appropriate volume of virus needed to be diluted in media to achieve desired MOI. MOI= [(Product Titer (IU/ml) x Virus Volume (ml))/(Total Cell Number)]. Read more about Multiplicity of Infection (MOI) at our learning resources center. 3. Prepare a mixture of complete media with polybrene at a concentration of 8 μg/ml. Remove the growth media from the wells and replace with 0.5 ml of the polybrene-media-mix per well (add in ViralPlus Transduction Enhancer (Cat. No. G515) at 1:100 or at your own optimized dilution ratio). 4. Once an effective MOI has been determined for the target cells through preliminary test infections, use the appropriate volume of virus to infect your cells. Leave one well of uninfected cells as an additional standard control. Following the infection, incubate the cells at 37°C with 5% CO2 overnight. 5. The optimal MOI for the lentivirus can range from 1-100 depending on promoter and cell types and we suggest an MOI ranging from 0.5-5 as a starting point. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV950 |
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- Xia, et al. "High levels of protein expression using different mammalian CMV promoters in several cell lines" Protein expression and Purification. 45(1):115-124 (2006).
- Wang, et al. "Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells" Scientific Reports : (2017).
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class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EAvailable Lentivirus Systems\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/adenovirus_expression_system\/Adeno-Expression-System-Summary-Table.png\u0022 alt=\u0022Available Lentivirus Expression Systems\u0022 width=\u0022100%\u0022 \/\u003E \u003Cbr \/\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EAvailable Adenovirus Systems\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003Cli class=\u0022col-sm-4 col-md-4 col-xs-6\u0022\u003E\n\u003Cdiv class=\u0022banners\u0022\u003E\n\u003Cdiv class=\u0022abm-category-workflow\u0022\u003E\u003Ca href=\u0022\/assets\/catalog\/category\/docs\/v\/i\/Viral-Vectors-Brochure-Single-Pages.pdf\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/VES.jpg\u0022 alt=\u0022Available Lentivirus Expression Systems\u0022 width=\u0022100%\u0022 gt=\u0022\u0022 br=\u0022\u0022 \/\u003E\u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EViral Vectors Brochure\u003C\/strong\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ch3\u003E\u003Cstrong\u003ECitations\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv\u003E\n\u003Ctable\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/ncomms9371\u0022\u003E01\u003C\/a\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003ELenzini, S. et al. (2020). \u0022Matrix mechanics and water permeation regulate extracellular vesicle transport.\u0022 Nature Nanotechnology. 15, 217-223. \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.nature.com\/articles\/s41565-020-0636-2\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1038\/s41565-020-0636-2\u003C\/a\u003E. \u003Cspan style=\u0022color: #000000;\u0022\u003EProduct: 2nd Generation Packaging System Mix (Cat.No LV003).\u003C\/span\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/?term=10.1038\/ncomms9371\u0022\u003E02\u003C\/a\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003EPapes, F. et al. (2022). \u0022Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content.\u0022 Nature Communications. 13(1).\u0026nbsp; \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022https:\/\/www.nature.com\/articles\/s41467-022-29942-w\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1038\/s41467-022-29942-w\u003C\/a\u003E\u003Ca href=\u0022https:\/\/www.nature.com\/articles\/s41467-022-29942-w\u0022\u003E.\u003C\/a\u003E \u003Cspan style=\u0022color: #000000;\u0022\u003EProduct: pLenti-Promoterless Vector (Cat.No LV013).\u003C\/span\u003E\u003C\/span\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Cspan class=\u0022citations-num\u0022\u003E03\u003C\/span\u003E\u003C\/td\u003E\n\u003Ctd\u003ECho, I et al. (2022). Deterministic Single Cell Encapsulation in Asymmetric Microenvironments to Direct Cell Polarity. Advanced Science. \u003Cspan style=\u0022color: #ef6331;\u0022\u003E\u003Ca href=\u0022https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/advs.202206014\u0022 style=\u0022color: #ef6331;\u0022\u003Edoi: 10.1002\/advs.202206014.\u003C\/a\u003E\u003C\/span\u003E Product: 2nd Generation Packaging System Mix (Cat.No LV003).\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Cloning Vectors \u0026 Packaging Mixes","meta_keywords":"viral packaging mix,lentivirus packaging mix,aav packaging mix,lentivirus packaging plasmids,aav packaging plasmids,aav serotype plasmids,lentiviral cloning vectors,aav,2nd generation lentivirus packaging mix,3rd generation lentivirus packaging mix,shuttle vectors,cloning vectors,viral cloning vectors","meta_description":"abm offers a variety of cloning vectors containing multiple cloning site for your specific insert. These vectors also come with various different promoters, reporters and tags for all your experimental needs.\u00a0","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":114,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":16624,"cat_no_base":"LV950","parent_id":602,"description":"\u003Cp\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s Lentivirus Promoter Blast\u0026trade; Kit is designed for easy monitoring and optimization of lentivirus transduction efficiency to help you achieve successful results in your stable cell line generation or engineering experiment. This kit includes 4 different GFP Lentiviruses (4 x 1ml at \u003E1x10\u003Csup\u003E6\u003C\/sup\u003E IU\/ml) with either the \u003Cstrong\u003ECMV, PGK, EF1\u0026alpha;, or UBC\u003C\/strong\u003E promoter, enabling you to determine which promoter works best for your cell line. In addition, this kit can be used to calculate the optimal Multiplicity of Infection (MOI) for your transduction experiment - simply transduce your cells at various MOIs and monitor GFP signals!\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EKit features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EEasy-to-use\u003C\/li\u003E\n\u003Cli\u003ESimplifies your transduction optimization process\u003C\/li\u003E\n\u003Cli\u003EHelps you draw accurate conclusions for which promoter is suitable for your cell line\u003C\/li\u003E\n\u003Cli\u003ESaves you time and resources\u003C\/li\u003E\n\u003Cli\u003EMammalian selection : Puromycin\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp\u003EAfter the optimal infection condition and promoter is identified, \u003Ca class=\u0022orange-link\u0022 href=\u0022\/catalog\/category\/view\/s\/lentivirus-library\/id\/206\/\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003Ethe lentivirus with your target gene and under your chosen promoter may be ordered separately\u003C\/a\u003E either in low or high titer format.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; width: 59.9097%;\u0022 border=\u00221\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022width: 27.2063%; text-align: center;\u0022\u003EProduct\u003C\/th\u003E\n\u003Cth style=\u0022width: 15.2157%; text-align: center;\u0022\u003EQuantity\u003C\/th\u003E\n\u003Cth style=\u0022width: 21.0049%; text-align: center;\u0022\u003ETiter (IU\/ml)\u003C\/th\u003E\n\u003Cth style=\u0022width: 25.896%; text-align: center;\u0022\u003ECat. Number\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd style=\u0022width: 27.2063%; text-align: center;\u0022\u003ELentivirus Promoter Blast\u0026trade; Kit\u003C\/td\u003E\n\u003Ctd style=\u0022width: 15.2157%; text-align: center;\u0022\u003E4 x 1ml\u003C\/td\u003E\n\u003Ctd style=\u0022width: 21.0049%; text-align: center;\u0022\u003E\u003E1x10\u003Csup\u003E6\u003C\/sup\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 25.896%; text-align: center;\u0022\u003ELV950\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cp\u003E\u003Cbr \/\u003E\u003Cstrong\u003EThe kit has a total of 4 different promoters in lentiviruses as listed below:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; width: 81.6128%; height: 144px;\u0022 border=\u00221\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022height: 44px;\u0022\u003E\n\u003Cth style=\u0022width: 42.7356%; height: 44px; text-align: center;\u0022\u003ELentivirus name\u003C\/th\u003E\n\u003Cth style=\u0022width: 6.56467%; text-align: center;\u0022\u003ESKU\u003C\/th\u003E\n\u003Cth style=\u0022width: 6.97497%; height: 44px; text-align: center;\u0022\u003EPromoter\u003C\/th\u003E\n\u003Cth style=\u0022width: 8.81419%; height: 44px; text-align: center;\u0022\u003EVolume (ml)\u003C\/th\u003E\n\u003Cth style=\u0022width: 8.58698%; height: 44px; text-align: center;\u0022\u003ETiter (IU\/ml)\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022width: 42.7356%; height: 25px; text-align: center;\u0022\u003E\u003Ca href=\u0022\/vector\/pLenti-III-CMV-GFP\u0022\u003ECMV Control Lentivirus (GFP)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.56467%; text-align: center;\u0022\u003E\u003Ca href=\u0022\/GFP-Control-Lentivector-Puro-LV008.html\u0022\u003ELV008\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.97497%; height: 25px; text-align: center;\u0022\u003ECMV\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.81419%; height: 25px; text-align: center;\u0022\u003E1\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.58698%; height: 25px; text-align: center;\u0022\u003E\u003E1x10\u003Csup\u003E6\u003C\/sup\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022width: 42.7356%; height: 25px; text-align: center;\u0022\u003E\u003Ca href=\u0022\/vector\/pLenti-III-PGK-GFP\u0022\u003EPGK Control Lentivirus (GFP)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.56467%; text-align: center;\u0022\u003E\u003Ca href=\u0022\/lenti-iii-pgk-gfp-control-virus-lv028.html\u0022\u003ELV028\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.97497%; height: 25px; text-align: center;\u0022\u003EPGK\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.81419%; height: 25px; text-align: center;\u0022\u003E1\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.58698%; height: 25px; text-align: center;\u0022\u003E\u003E1x10\u003Csup\u003E6\u003C\/sup\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022width: 42.7356%; height: 25px; text-align: center;\u0022\u003E\u003Ca href=\u0022\/vector\/pLenti-III-EF1alpha-GFP\u0022\u003EEF1\u0026alpha; Control Lentivirus (GFP)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.56467%; text-align: center;\u0022\u003E\u003Ca href=\u0022\/lenti-iii-ef1alpha-gfp-control-virus-lv046.html\u0022\u003ELV046\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.97497%; height: 25px; text-align: center;\u0022\u003EEF1\u0026alpha;\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.81419%; height: 25px; text-align: center;\u0022\u003E1\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.58698%; height: 25px; text-align: center;\u0022\u003E\u003E1x10\u003Csup\u003E6\u003C\/sup\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr style=\u0022height: 25px;\u0022\u003E\n\u003Ctd style=\u0022width: 42.7356%; height: 25px; text-align: center;\u0022\u003E\u003Ca href=\u0022\/vector\/pLenti-III-Ubc-GFP\u0022\u003EUbC Control Lentivirus (GFP)\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.56467%; text-align: center;\u0022\u003E\u003Ca href=\u0022\/lenti-iii-ubc-gfp-control-virus-lv027.html\u0022\u003ELV027\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd style=\u0022width: 6.97497%; height: 25px; text-align: center;\u0022\u003EUbC\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.81419%; height: 25px; text-align: center;\u0022\u003E1\u003C\/td\u003E\n\u003Ctd style=\u0022width: 8.58698%; height: 25px; text-align: center;\u0022\u003E\u003E1x10\u003Csup\u003E6\u003C\/sup\u003E\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","disclaimer":"\u003Ca href=\u0022\/terms#disclaimer-section\u0022\u003EClick for more info\u003C\/a\u003E","application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":"","population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"\/l\/e\/lentivirus-promoter-blast-kit_data.jpg","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":null,"caution":null,"storage_condition":null,"product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"LV950 Box","note":"\u003Cp\u003E\u003Cstrong\u003EInfection Protocol\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003E1. Plate the target cells in a 24-well plate, 24 hours prior to viral infection at a density of 0.5\u0026times;10\u003Csup\u003E5\u003C\/sup\u003E cells per well. Add 0.5 ml of complete optimal medium (with serum and antibiotics if required) and incubate the cells at 37\u0026deg;C with 5% CO\u003Csub\u003E2\u003C\/sub\u003E overnight.\u003C\/p\u003E\n\u003Cp\u003E\u003Cem\u003ENote: It is possible to use other plate formats for transduction. In this case, the amount of cells should be adjusted depending on the growth area of the well\/plate.\u003C\/em\u003E\u003C\/p\u003E\n\u003Cp\u003E2. Thaw the lentivirus from \u0026ndash;80\u0026deg;C at room temperature. We strongly suggest to make usable aliquots of lentivirus to avoid free-thaw since that is known to reduce the viral titer.Calculate the appropriate volume of virus needed to be diluted in media to achieve desired MOI. MOI= [(Product Titer (IU\/ml) x Virus Volume (ml))\/(Total Cell Number)]. Read more about \u003Ca class=\u0022orange-link\u0022 href=\u0022https:\/\/info.abmgood.com\/multiplicity-of-infection-moi\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003EMultiplicity of Infection (MOI) at our learning resources center\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E3. Prepare a mixture of complete media with polybrene at a concentration of 8 \u0026mu;g\/ml. Remove the growth media from the wells and replace with 0.5 ml of the polybrene-media-mix per well (add in ViralPlus Transduction Enhancer (Cat. No. \u003Ca class=\u0022orange-link\u0022 href=\u0022\/viralentry-transduction-enhancer.html#viralplus\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003EG515\u003C\/a\u003E) at 1:100 or at your own optimized dilution ratio).\u003C\/p\u003E\n\u003Cp\u003E4. Once an effective MOI has been determined for the target cells through preliminary test infections, use the appropriate volume of virus to infect your cells. Leave one well of uninfected cells as an additional standard control. Following the infection, incubate the cells at 37\u0026deg;C with 5% CO\u003Csub\u003E2\u003C\/sub\u003E overnight.\u003C\/p\u003E\n\u003Cp\u003E5.\u0026nbsp;The optimal MOI for the lentivirus can range from 1-100 depending on promoter and cell types and we suggest an MOI ranging from 0.5-5 as a starting point.\u003C\/p\u003E","recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":null,"cas_origin":null,"cas_protein_marker":null,"source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-15 07:17:35","updated_at":"2025-03-09 18:54:29","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379253,"parent_id":602,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/l\/e\/lentivirus-promoter-blast-kit_data.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":4,"status":0,"entity_id_m2":18081036,"sku_in_m2":"LV950","value_id_m2":49912288,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:39:28"}],"gene":null}