OneScript® Plus Reverse Transcriptase
Cat. No.
G237
Unit
100 rxn
Price
$129.00
| Cat. No. | G237 | ||||||||
| Name | OneScript® Plus Reverse Transcriptase | ||||||||
| Unit | 100 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Application |
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G237 |
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
|
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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| What can I use the synthesized cDNA for? | |
|
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
|
Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
|
Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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| What volumes of cDNA should I use for downstream PCR? | |
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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What if input RNA samples are expected to have high levels of genomic DNA contamination?
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If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
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Do I need an RNase inhibitor when using this product?
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All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
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| What discontinued abm products is Cat. No. G237 equivalent to? | |
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This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript® Two-Step RT-PCR Kits and OneScript® Plus Two-Step RT-PCR Kits). Contact our customer service team technical@abmgood.com for more information.
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Arroyave, F., Uscátegui, Y., & Lizcano, F. (2024). From iPSCs to pancreatic β cells: unveiling molecular pathways and enhancements with vitamin C and retinoic acid in diabetes research. International Journal of Molecular Sciences, 25(17), 9654. https://doi.org/10.3390/ijms25179654
Ates, A., Tastan, C., & Ermertcan, S. (2024). CRISPR-Cas9-Mediated Targeting of Multidrug Resistance Genes in Methicillin-Resistant Staphylococcus aureus. The CRISPR Journal, 7(6), 374-384. https://doi.org/10.1089/crispr.2024.0001
Çarman, K. B., Tekin, H. G., Çavuşoğlu, D., Yarar, C., Kaplan, E., Karademir, C. N., & Arslantaş, D. (2023). Evaluation of microRNAs in pediatric epilepsy. Turkish archives of pediatrics, 58(4), 429. https://doi.org/10.5152/TurkArchPediatr.2023.22320
Gallardo, C. M., Wang, S., Montiel-Garcia, D. J., Little, S. J., Smith, D. M., Routh, A. L., & Torbett, B. E. (2021). MrHAMER yields highly accurate single molecule viral sequences enabling analysis of intra-host evolution. Nucleic acids research, 49(12), e70-e70. https://doi.org/10.1093/nar/gkab231
Gutiérrez-Gómez, M. L., Ruíz, Z., Gamboa, F., Roa, N. S., Cardozo, C., Ariza, B., ... & García-Robayo, D. A. (2024). SARS-CoV-2 diagnosis in saliva samples: Usefulness and limitations. Diagnostic Microbiology and Infectious Disease, 109(3), 116320. https://doi.org/10.1016/j.diagmicrobio.2024.116320
Han, J., Zhao, Z., Zhang, N., Yang, Y., Ma, L., Feng, L., ... & Wang, G. (2021). Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism. Aging (Albany NY), 13(4), 5034. https://doi.org/10.18632/aging.202414
Han, J., Zuo, J., Zhang, X., Wang, L., Li, D., Wang, Y., ... & Feng, L. (2022). TRIM29 is differentially expressed in colorectal cancers of different primary locations and affects survival by regulating tumor immunity based on retrospective study and bioinformatics analysis. Journal of Gastrointestinal Oncology, 13(3), 1132. https://doi.org/10.21037/jgo-22-365
Karki, D., LaPointe, A. T., Isom, C., Thomas, M., & Sokoloski, K. J. (2025). Mechanistic insights into Sindbis virus infection: noncapped genomic RNAs enhance the translation of capped genomic RNAs to promote viral infectivity. Nucleic Acids Research, 53(1), gkae1230. https://doi.org/10.1093/nar/gkae1230
Kilercioglu, S., Atli, G., Evliyaoglu, E., Buyukdeveci, M. E., Aydın, F., Sangun, L., ... & Ozdes, A. (2023). The effects of 100 nm pure boron nanoparticles on the primary antioxidant system of Nile tilapia (Oreochromis niloticus). Chemosphere, 339, 139727. https://doi.org/10.1016/j.chemosphere.2023.139727
Liman, R., Kursunlu, A. N., Ozmen, M., Arslan, S., Mutlu, D., Istifli, E. S., & Acikbas, Y. (2022). Synthesis of water soluble symmetric and asymmetric pillar [5] arene derivatives: Cytotoxicity, apoptosis and molecular docking studies. Journal of Molecular Structure, 1265, 133482. https://doi.org/10.1016/j.molstruc.2022.133482
Punzi, S., Balestrieri, C., D’Alesio, C. et al. WDR5 inhibition halts metastasis dissemination by repressing the mesenchymal phenotype of breast cancer cells. Breast Cancer Res 21, 123 (2019). https://doi.org/10.1186/s13058-019-1216-y
Smith, T. K., Kahiel, Z., LeBlond, N. D., Ghorbani, P., Farah, E., Cote, M., ... & Fullerton, M. D. (2019). Characterization of LXR-activating nanoparticle formulations in primary mouse macrophages. bioRxiv, 746784. http://dx.doi.org/10.3390/molecules24203751
Téllez Valerio, C.E., Gregorio Jorge, J., Luna Suárez, S. et al. MiR6024 overexpression increases the susceptibility of Nicotiana tabacum to Sclerotinia sclerotiorum. Eur J Plant Pathol 165, 97–113 (2023). https://doi.org/10.1007/s10658-022-02591-x
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Simply add your RNA sample with our mastermix and incubate.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-workflow.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EAccurate cDNA Synthesis\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EGenomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022col-sm-12\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-all-in-one-product-figure.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-gray abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Hot RT\u0022 id=\u0022OneScriptHot\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-div\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ESynthesize cDNA from complex RNA templates with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase. Engineered for outstanding thermostability, our OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase is ideal for handling secondary structures (e.g. GC-rich templates) as well as long, degraded, or contaminated RNA samples, making it the superior choice for challenging RNA templates.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 50px;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/extreme-thermostability-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EExtreme thermostability at 50-80\u0026deg;C\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/transcribes-degraded-contaminated-samples-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003ETranscribes degraded samples and resists inhibitors \/ contaminants\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/high-cDNA-yields-icon.png\u0022 \/\u003E\n\u003Cp style=\u0022font-size: 15px;\u0022\u003EHigh cDNA yields from difficult or ultra-low RNA samples\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-4 col-lg-4\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptases-onescript-hot-product-image-large.png\u0022 \/\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022 style=\u0022--first-child-width: 50%;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth style=\u0022text-align: left;\u0022\u003EProduct Name\u003C\/th\u003E\n\u003Cth\u003ECat. No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003E\u003C\/a\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EAll-In-One 5X RT MasterMix with gDNA Removal\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/all-in-one-5x-rt-mastermix.html\u0022\u003EG592\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G592\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EOneScript\u0026reg; Hot 5X RT MasterMix\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-hot-rt-mastermix.html\u0022\u003EG590\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G590\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EOneScript\u0026reg; Hot cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-cdna-synthesis-kit.html#G594\u0022\u003EG594\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G594\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EOneScript\u0026reg; Hot Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescriptr-hot-reverse-transcriptase.html#G593\u0022\u003EG593\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G593\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EMegaFi\u0026trade; One-Step RT-PCR\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/megafi-one-step-rt-pcr.html\u0022\u003EG597\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G597\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EOutstanding Thermostability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-hot-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase has exceptional thermostability. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot Reverse Transcriptase (Cat. No. G593) was used in a 10 min. reaction with RNA template at temperatures ranging from 50 and 80\u02daC. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr abm-page-menu\u0022 data-text=\u0022OneScript\u0026reg; Plus RT\u0022 id=\u0022OneScriptPlus\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-xs-12 col-sm-12 col-md-8 col-lg-8\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022 style=\u0022text-align: justify;\u0022\u003ERoutine cDNA synthesis can be completed in just 10-15 minutes with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase! 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No.\u003C\/th\u003E\n\u003Cth\u003ESize\u003C\/th\u003E\n\u003Cth\u003EPrice\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-reverse-transcriptase.html\u0022\u003EOneScript\u0026reg; Plus Reverse Transcriptase\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-mmlv-reverse-transcriptase.html\u0022\u003EG237\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G237\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EOneScript\u0026reg; Plus cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022\u003EG236\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E100 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G236\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EmiRNA All-In-One cDNA Synthesis Kit\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E\u003Ca class=\u0022orange-link\u0022 href=\u0022\/mirna-all-in-one-cdna-synthesis-kit.html\u0022\u003EG898\u003C\/a\u003E\u003C\/td\u003E\n\u003Ctd\u003E20 rxn\u003C\/td\u003E\n\u003Ctd class=\u0022product-price\u0022 data-product=\u0022G898\u0022\u003EInquiry\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Ch4 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-description-title\u0022\u003E\u003Cstrong\u003EExcellent Elongation Ability\u003C\/strong\u003E\u003C\/h4\u003E\n\u003Cbr \/\u003E\n\u003Cdiv class=\u0022row\u0022\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/reverse-transcriptase-onescript-plus-data-1.png\u0022 \/\u003E\u003C\/div\u003E\n\u003Cdiv class=\u0022col-sm-6\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase can elongate RNA templates up to 15 kb in length. \u003C\/strong\u003E OneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase (Cat. No. G237) was used in a reaction with a range of human RNA fragments. The resulting synthesized cDNA was followed by PCR and visualized on a 1% agarose gel.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-mt-5\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003EUnsure which reverse transcriptase is right for you?\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003EUnsure which reverse transcriptase is right for your application? \u003Ca class=\u0022orange-link\u0022 href=\u0022mailto: technical@abmgood.com\u0022\u003EContact us\u003C\/a\u003E for assistance or use the simple RT Enzyme Selection Guide below to compare the characteristics of each reverse transcriptase.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022table-responsive\u0022\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table1\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth width=\u002240%\u0022\u003ECHARACTERISTIC\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptHot\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Hot\u003C\/a\u003E\u003C\/th\u003E\n\u003Cth width=\u002230%\u0022\u003E\u003Ca href=\u0022#OneScriptPlus\u0022 class=\u0022abm-page-category-target-link\u0022 style=\u0022color: white;\u0022\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus\u003C\/a\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctbody\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EThermostability\u003C\/td\u003E\n\u003Ctd\u003E50-80\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50-55\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOptimal Temperature\u003C\/td\u003E\n\u003Ctd\u003E60\u0026deg;C\u003C\/td\u003E\n\u003Ctd\u003E50\u0026deg;C\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMaximum cDNA Elongation\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003Ctd\u003E15 kb\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EMinimum Sample Input\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003Ctd\u003E0.1 pg\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EReaction Time\u003C\/td\u003E\n\u003Ctd\u003E10 min\u003C\/td\u003E\n\u003Ctd\u003E15 min\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EInhibitor\/Contamination Resistance\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003ETolerance to Complex Templates\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003Ctd\u003E\u0026bull;\u0026bull;\u0026bull;\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022tr-white abm-custom-page-tr\u0022 id=\u0022free_sample\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022\u003E\u003Cstrong\u003ERequest a Free Sample\u003C\/strong\u003E\u003C\/h3\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-custom-page-block-div\u0022\u003E\n\u003Cdiv class=\u0022\u0022\u003E\u003Chr class=\u0022abm-custom-page-hr\u0022 \/\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left\u0022\u003E\n\u003Cdiv class=\u0022abm-custom-page-align-left abm-custom-p\u0022\u003E\n\u003Cp class=\u0022abm-custom-page-p\u0022\u003ESee for yourself why your fellow RT-PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a \u0022first come first served\u0022 basis and shipping charges may apply.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Ciframe style=\u0022min-width: 100%; height: 1400px; border: none;\u0022 id=\u0022JotFormIFrame-201957212407249\u0022 title=\u0022Free Sample Request\u0022 allowtransparency=\u0022true\u0022 allowfullscreen=\u0022allowfullscreen\u0022 allow=\u0022geolocation; microphone; camera\u0022 src=\u0022https:\/\/form.jotform.com\/201957212407249\u0022 frameborder=\u00220\u0022 scrolling=\u0022no\u0022\u003E\n \u003C\/iframe\u003E\n\u003Ch3 class=\u0022abm-custom-page-h1 abm-custom-page-align-left abm-custom-page-product-name\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\u003Cstrong\u003EResource\u003C\/strong\u003E\u003C\/h3\u003E\n\u003Cdiv class=\u0022row\u0022 style=\u0022margin-top: 1em;\u0022\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-reverse-transcription\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-PCR_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EReverse Transcription\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022https:\/\/info.abmgood.com\/polymerase-chain-reaction-pcr-variations\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/catalogPage\/rtpcr\/Resources_Reverse-Transcription-Variations-to-the-system_subcat.png\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EVariations to the System\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EPCR Knowledge Base\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-catalogpage-box col-sm-4\u0022\u003E\u003Ca href=\u0022\/pcr-competitor-cross-reference-tool\u0022\u003E \u003Cimg src=\u0022\/assets\/images\/category\/wysiwyg\/PCR-Product-Comparison-Tool-Homepage-Banner.png\u0022 width=\u0022364\u0022 height=\u0022182\u0022 \/\u003E \u003C\/a\u003E\n\u003Cdiv class=\u0022abm-category-image-title\u0022\u003E\u003Cstrong\u003EPCR\/qPCR Competitor Cross Reference Tool\u003C\/strong\u003E \u003Cbr \/\u003E\n\u003Cdiv style=\u0022font-size: 0.8em;\u0022\u003E\u003Ci\u003EProduct comparison\u003C\/i\u003E\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cscript\u003E\n const catNoList = [];\n document.querySelectorAll(\u0027.product-price\u0027).forEach(el =\u003E {\n if (el.dataset.product) {\n el.innerHTML = \u0027Inquiry\u0027;\n if (el.dataset.type) {\n catNoList.push({\n type: el.dataset.type,\n value: el.dataset.product,\n });\n } else {\n catNoList.push(el.dataset.product);\n }\n }\n });\n fetch(\u0027\/product\/geoPrice\u0027, {\n method: \u0027post\u0027,\n body: JSON.stringify({ cat_no: catNoList, _token: document.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027).content }),\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n }\n }).then(res =\u003E {\n const json = res.json();\n json.then(response =\u003E {\n if (response.code === 0) {\n const result = response.result;\n for (const key in result) {\n document.querySelectorAll(\u0027.product-price[data-product=\u0022\u0027 + key + \u0027\u0022]\u0027).forEach(el =\u003E {\n el.innerHTML = \u0027$\u0027 + result[key];\n });\n }\n }\n });\n });\n \u003C\/script\u003E\n\u003C\/div\u003E","meta_title":"Reverse Transcription \u0026 RT-PCR","meta_keywords":"Reverse Transcription \u0026 RT-PCR","meta_description":"Reverse Transcription \u0026 RT-PCR","deleted_at":null,"enable":"Y","parent_list":"27","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":103,"in_footer":1,"fid":27,"created_at":null,"updated_at":"2025-04-02 01:04:45"},"info":{"id":693,"cat_no_base":"G237","parent_id":36,"description":"\u003Cp\u003E\u003Cstrong\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus Reverse Transcriptase\u003C\/strong\u003E is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50\u0026deg;C - 55\u0026deg;C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.\u003C\/p\u003E\n\u003Cp\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus is formulated with \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0026rsquo;s RNaseOFF Ribonuclease Inhibitor (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/rnaseoff-ribonuclease-inhibitor.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G138\u003C\/a\u003E) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (\u003C 1 mM), making it the best choice for ultimate RNA protection.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EProduct Features:\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cul\u003E\n\u003Cli\u003EHigh thermostability at 50-55\u0026deg;C\/122-131\u0026deg;F\u003C\/li\u003E\n\u003Cli\u003ESynthesize 15 kb RNA Templates\u003C\/li\u003E\n\u003Cli\u003E10-15 minutes reaction time\u003C\/li\u003E\n\u003Cli\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E Plus cDNA Synthesis Kit (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/onescript-plus-cdna-synthesis-kit.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G236\u003C\/a\u003E) also available\u003C\/li\u003E\n\u003Cli\u003EPair with BlasTaq\u0026trade; 2X PCR MasterMix (\u003Ca class=\u0022orange-link\u0022 href=\u0022\/blastaq-2x-pcr-taq-mastermix.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener\u0022\u003ECat. No. G895\u003C\/a\u003E) for Two-Step RT-PCR workflows\u003C\/li\u003E\n\u003Cli\u003EIncluded in \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s \u003Ca href=\u0022\/pcr-buffet-program\u0022\u003EPCR Buffet Program\u003C\/a\u003E\u003C\/li\u003E\n\u003C\/ul\u003E\n\u003Cp style=\u0022margin: 30px 0;\u0022\u003E\u003Ca href=\u0022\/free-sample\u0022\u003E\u003Cimg src=\u0022\/assets\/images\/\/wysiwyg\/Request-Free-Sample-Button.png\u0022 alt=\u0022Request Free Sample\u0022 width=\u0022204\u0022 height=\u002245\u0022 \/\u003E\u003C\/a\u003E\u003C\/p\u003E\n\u003Ctable class=\u0022bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\u0022 style=\u0022margin-bottom: 20px;\u0022\u003E\n\u003Cthead\u003E\n\u003Ctr\u003E\n\u003Cth\u003E\u003Cstrong\u003EProduct Component\u003C\/strong\u003E\u003C\/th\u003E\n\u003Cth\u003E\u003Cstrong\u003EQuantity\u003C\/strong\u003E\u003C\/th\u003E\n\u003C\/tr\u003E\n\u003C\/thead\u003E\n\u003Ctfoot\u003E\n\u003Ctr\u003E\n\u003Ctd\u003EOneScript\u003Csup\u003E\u0026reg;\u003C\/sup\u003E\u0026nbsp;Plus Reverse Transcriptase\u003C\/td\u003E\n\u003Ctd\u003E100 rxn (100 \u0026micro;l)\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003Ctr\u003E\n\u003Ctd\u003E5X RT Buffer\u003C\/td\u003E\n\u003Ctd\u003E400 \u0026micro;l\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tfoot\u003E\n\u003C\/table\u003E\n\u003Ctable style=\u0022border-collapse: collapse; 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