Recombinant Human Heat-Stable FGF2 (E. coli)
| Cat. No. | Z103457 |
| Name | Recombinant Human Heat-Stable FGF2 (E. coli) |
| Description |
abm’s Recombinant Human Heat-Stable FGF2 is a stabilized form of FGF2 designed to provide more consistent performance under cell culture conditions where prolonged incubation can reduce growth factor activity. Its enhanced thermal stability helps support compact colony morphology, defined colony borders, and uniform cell appearance during pluripotent stem cell culture.
FGF2 is an important growth factor involved in the regulation of cell growth, proliferation, differentiation, and survival, and is widely used for the maintenance and expansion of pluripotent stem cells.
In stem cell culture studies using medium pre-incubated at 37°C, abm Heat-Stable FGF2 maintained strong colony morphology and apparent colony quality compared with wild-type FGF2 and performed comparably to or better than a commercial heat-stable FGF2 control. |
| Organism | Human (H. sapiens) |
| Unpacking and Storage Instructions |
Upon receipt, immediately store the lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, aliquot the protein as needed and avoid repeated freeze/thaw cycles. Reconstituted protein may be stored at 2–8°C for up to 1 month or at –20°C for up to 6 months. |
| Storage Condition | Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Unit quantity | 100 µg |
| Family | FGF Family |
| Tag | Tag Free |
| Aliases |
BFGF, bFGF, FGF-2, FGF2, HBGF-2, Heat-Stable FGF2, HS-FGF2, Thermostable FGF2 |
| Gene Symbol | FGF2 |
| Gene ID | 2247 |
| Accession Number | P09038 |
| Source | E.coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | Approximately 17 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS |
| Function | FGF2 is an important growth factor involved in the regulation of cell proliferation, growth, differentiation, and survival. Recombinant Human Heat-Stable FGF2 is designed for enhanced stability under cell culture conditions and supports the maintenance and expansion of pluripotent stem cells, helping preserve compact colony morphology and uniform cell growth during prolonged incubation. |
| Reconstitution | Briefly centrifuge the vial before opening. Reconstitute the lyophilized protein in sterile distilled water to a concentration of at least 0.1 mg/mL. Filter sterilization following reconstitution is required. The reconstituted protein can then be further diluted into the desired buffer or culture medium. |
| Shipping Conditions | Ice Pack |
| Storage | Upon receipt, store the lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z103457 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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