Cas9 Expressing 293T Cell Line

{"id":38131,"cat_no":"T3251","retired_cat_no":"","old_cat_no":"T3251","name":"Cas9 Expressing 293T Cell Line","price":"2000.0000","price_before_discount":"0.0000","academic_price":"795.00","commercial_price":"0.00","distributor_price_note":"","unit_quantity":"1x10\u003Csup\u003E6\u003C\/sup\u003E cells \/ 1.0 ml","category_id":0,"category_name":"Stable Cell Lines","frontend_category_id":25,"frontend_category_ids":"20,21,25","gene_id":0,"ncbi_id":0,"gene_name":"","gene_full_name":"","alias":"","accession_number":"","species_id":0,"species":"Human (H. sapiens)","real_species_id":0,"cell_type_id":0,"cell_type":"Stable Cell Lines","product_type":"","tissue_id":0,"tissue":"Kidney","tissue_system":"","source":null,"keywords":"293T cell line, 293t cells, 293 t cell line, 293t, 293 t, 293, 293 t cells, 293 cells, 293 cell line, stable 293T cell line, stable 293T cells, stable cell line, human cell line, human cells, stable human cells, stable human cell line, immortalized cell line, HEK293T, hek293 t, hek293, human embryonic kidney cells, kidney cell line, hek, SV40, SV40T, T-antigen, T antigen, stable 293T, cas9, cas 9, cas-9, cas expressing, cas","invisible_search_keywords":"TMX2-CTNND1 cell","filter_group_id":13,"filter_group_name":"Cell Lines","filter_name":"Stable Cell Lines","search_filter_ids":"41,44,48,102,108","search_filter_keys":"41-44-48,102-108","temp_field":null,"filter_id":63,"customization":0,"filter_table_id":9417,"filter_table_name":"","related_group_id":0,"operator":"","status":1,"is_ko":0,"need_remove":0,"units":null,"document_sort":"","price_old":"0.0000","price2024":"0.0000","created_at":null,"updated_at":"2025-03-29 00:30:18","points_rule":5,"Confirmed":"Tony Confirmed","application_no_use":null,"application_id_no_use":null,"raised_in":null,"raised_in_id":null,"geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"","frontendBreadcrumbs":[{"id":20,"pid":86,"name":"Cellular Materials","page_id":18,"page_type":"App\\Models\\Category","href":"","sort":20,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"cellular-materials.html","url":"cellular-materials.html"},{"id":21,"pid":20,"name":"Cell Library Collections","page_id":221,"page_type":"App\\Models\\Category","href":null,"sort":21,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":"2025-02-12 01:11:16","url_key":"cellular-collections.html","url":"cellular-collections.html"},{"id":25,"pid":21,"name":"Stable Cell Lines","page_id":20,"page_type":"App\\Models\\Category","href":null,"sort":25,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"Stable-Cell-Lines.html","url":"Stable-Cell-Lines.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseCells","info_family":"","geo_price":"2,000.00","geo_price_symbol":"$2,000.00","category":null,"info":{"id":5199,"cat_no_base":"T3251","parent_id":38131,"image":"\/t\/3\/t3251-western-blot-analysis-for-cas9.jpg","price-original":null,"description":"\u003Cp\u003EThis cell line stably expresses spCas9 Nuclease.\u0026nbsp;\u003C\/p\u003E","shipment_type":null,"internal_leadtime":"0","lysate_type":null,"small_image":"\/t\/3\/t3251-western-blot-analysis-for-cas9.jpg","growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003EThis cell line stably expresses spCas9 Nuclease.\u0026nbsp;\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"DMEM + 10% FBS + 1% P\/S + 0.8 \u00b5g\/ml puromycin (G264)","alias":null,"tier_price":null,"operator_no_use":"tony_liu","addtime":"2016-05-13 00:00:00","cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":null,"expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":"0","cell_morphology_specification":null,"tax_class_id":2,"inventory_location":"New G7 (E2-E9, F1-F2)","vector":null,"internal_note":"\u003Cp\u003EpLenti-EF1a-Cas9-Puro\u003C\/p\u003E\n\u003Cp\u003E2023-11-21: some cells died with puromycin addition for cas9 QC\u003C\/p\u003E\n\u003Cp\u003E2023-12-18: Monoclones generated\u003C\/p\u003E\n\u003Cp\u003E************************************** Lot Passage No. *************************************\u003C\/p\u003E\n\u003Cp\u003E0195845445002 (poly-clone lot):P = 21 |\u0026nbsp;C23D14BA: P=U+8 |\u0026nbsp;C23D14BA: P=U+8\u003C\/p\u003E\n\u003Ctable width=\u00220\u0022\u003E\u003Ccolgroup\u003E\u003Ccol style=\u0022width: 212px;\u0022 width=\u0022212\u0022 \/\u003E\u003C\/colgroup\u003E\u003C\/table\u003E\n\u003Cp\u003EB24M16OCB: P= P1 \u0026nbsp; \u0026nbsp;\u003C\/p\u003E\n\u003Ctable width=\u00220\u0022\u003E\u003Ccolgroup\u003E\u003Ccol style=\u0022width: 212px;\u0022 width=\u0022212\u0022 \/\u003E\u003C\/colgroup\u003E\u003C\/table\u003E\n\u003Cp\u003EB24S27BAN: P= U+P3 \u0026nbsp; \u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":null,"seeding_density_ml":null,"source":null,"source_catno":"\u003Cp\u003E560\/2160 (commercial price - price match Genecopoeia)\u003C\/p\u003E","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":null,"source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":"ABM","donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":"Cas9-Expressing 293T Cell Line Western blot","biological_activity_text":null,"cart2quote_quotable":"2","biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":"178,179","isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003EAdherent, polygonal\u003C\/p\u003E","expression_profile":null,"storage":null,"propagation_method":"\u003Cp\u003EUse of PriCoat\u0026trade; T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow III (TM003) + 10% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u0026deg;C, 5% CO\u2082. Selection with 0.6 \u0026micro;g\/ml Puromycin (G264)\u003C\/p\u003E","usage":null,"freeze_thaw_recovery":"1. Pre-warm complete media in a 37\u0026deg;C waterbath.\u003Cbr\u003E\n2. Remove the cryopreserved vial from the liquid nitrogen storage tank.\u003Cbr\u003E\n3. Thaw the cells quickly by placing the lower half of the vial into the 37\u0026deg;C water bath and remove after 60 seconds. There should still be a few ice crystals left after thawing. It is important not to over-thaw the cryovials as the presence of DMSO is toxic to the cells.\u003Cbr\u003E\n4. Re-suspend the cells in the vial and transfer the cell suspension into a 15mL sterile conical tube containing 5mL complete media.\u003Cbr\u003E\n5. Centrifuge cells at 1500rpm for 3 minutes to pellet.\u003Cbr\u003E\n6. Aspirate out the media, leaving cell pellet undisturbed.\u003Cbr\u003E\n7. Re-suspend pellet in fresh culture medium and plate in new culture vessel.\u003Cbr\u003E\n8. Incubate cultures at 37\u0026deg;C, 5% CO\u003Csub\u003E2\u003C\/sub\u003E.","str_profile":null,"str_markers":"","subculture_protocol":"\u003Cp\u003EVolumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.\u003C\/p\u003E\n\u003Cp\u003E1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.\u003C\/p\u003E\n\u003Cp\u003E2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u0026deg;C, for several minutes to facilitate detachment.\u003C\/p\u003E\n\u003Cp\u003E3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.\u003C\/p\u003E\n\u003Cp\u003E4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.\u003C\/p\u003E\n\u003Cp\u003E5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.\u003C\/p\u003E\n\u003Cp\u003E6. Incubate the cells at the recommended conditions.\u003C\/p\u003E","preservation_protocol":null,"passage_number":null,"qc":null,"shipping_conditions":"\u003Cp\u003EShip with dry ice.\u003C\/p\u003E","disclaimer_general":"\u003Cp\u003E1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and procedures. The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period.\u0022\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":"ABM","internal_product_note":null,"donor_history":"Embryo","split_ratio":null,"expression":"\u003Cp\u003ECas9 (pLenti-EF1a-Cas9-Puro)\u003C\/p\u003E","growth_conditions":"\u003Cp\u003E\u003Cstrong style=\u0022color: rgb(255, 0, 0); text-wrap: wrap;\u0022\u003E\u003Cstrong\u003EPriCoat\u2122 T25 Flasks (\u003Ca href=\u0022https:\/\/www.abmgood.com\/pricoat-t25-flasks.html\u0022 target=\u0022_self\u0022\u003EG299\u003C\/a\u003E) or Applied Cell Extracellular Matrix (\u003Ca href=\u0022https:\/\/www.abmgood.com\/applied-cell-extracellular-matrix-g422.html\u0022 target=\u0022_self\u0022\u003EG422\u003C\/a\u003E) are recommended for optimal cell culture.\u003C\/strong\u003E\u0026nbsp;\u003C\/strong\u003E Dulbecco\u0027s Modified Eagle Medium (DMEM), High Glucose (\u003Ca href=\u0022https:\/\/www.abmgood.com\/Dulbecco-s-Modified-Eagle-Medium-DMEM-,-High-Glucose.html\u0022 target=\u0022_self\u0022\u003ETM500\u003C\/a\u003E) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (\u003Ca href=\u0022https:\/\/www.abmgood.com\/penicillin-streptomycin-solution-g255.html\u0022 target=\u0022_self\u0022 style=\u0022text-wrap: wrap;\u0022\u003EG255\u003C\/a\u003E), 37.0\u00b0C, 5% CO\u2082.\u0026nbsp;\u003C\/p\u003E\u003Cbr\u003E\u003C\/br\u003E\u003Cp\u003E0.8 \u00b5g\/ml Puromycin (\u003Ca href=\u0022https:\/\/www.abmgood.com\/puromycin-g264.html\u0022 target=\u0022_self\u0022\u003EG264\u003C\/a\u003E) for selection. \u003Cbr\u003E\u003C\/br\u003E\nNote: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. \u003C\/p\u003E\u003Cp\u003E\u003Ci\u003E*Do not heat-inactivate\u003C\/i\u003E\u003C\/p\u003E","recommend":"\u003Cp\u003EWorried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":null,"cryopreservation":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003ECryopreservation Medium (\u003C\/span\u003E\u003Ca style=\u0022text-wrap: wrap;\u0022 href=\u0022https:\/\/www.abmgood.com\/cryopreservation-medium-tm024.html\u0022 target=\u0022_self\u0022\u003ETM024\u003C\/a\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E), or complete growth media with 10% DMSO.\u003C\/span\u003E\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","promotions":null,"sync_images":null,"thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-15 06:26:43","updated_at":"2025-03-29 00:30:18","concentration":null},"seo":{"id":66998,"catalog_id":38131,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"cas9-expressing-293t-cell-line.html","meta_title":"Cas9 Expressing 293T Cell Line | abm","meta_keywords":"293T cell line, 293t cells, 293 t cell line, 293t, 293 t, 293, 293 t cells, 293 cells, 293 cell line, stable 293T cell line, stable 293T cells, stable cell line, human cell line, human cells, stable human cells, stable human cell line, immortalized cell l","meta_description":"Order Cas9 Expressing 293T Cell Line from abm, a stable cell line that expresses spCas9 nuclease for CRISPR genome editing. Price: Inquiry. Request a quote today and get cell line insurance for peace of mind.","created_at":"2022-09-23 06:53:13","updated_at":"2024-05-13 19:44:57"},"maps":[],"media":[{"id":388767,"parent_id":38131,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/Ky4jlfxLjsOCpI7Qg94SAqqfC83sqq2DSPDWKDAl.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-29 00:30:05","updated_at":"2025-03-29 00:30:05"},{"id":388768,"parent_id":38131,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/21CHRQEkiTklPc10e0B1UDwuxDmjCZUeDfZ1Pwj0.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-29 00:30:13","updated_at":"2025-03-29 00:30:13"}],"gene":null}