Worried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under abm's Cell Line Insurance.
Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.
For for-profit organizations, please contact quotes@abmgood.com for pricing.
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
BioSafety
II
Certificate of Analysis
For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.
Growth Conditions
PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS(Regular*) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
0.4 µg/ml Puromycin (G264) for selection.
Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.
*Do not heat-inactivate
Unpacking and Storage Instructions
1. Visually examine the packaging containers for signs of leakage or breakage.
2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use.
To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.
Thawing Protocol
1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.
5. Incubate the cells at the recommended conditions.
Subculture Protocol
1. Simply add fresh complete media directly to the culture. Do not allow cell density to exceed 1x10⁶ cells/ml.
2. Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired.
3. Incubate the cells at the recommended conditions.
Cryopreservation
Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.
Seeding Density (cells/ml)
100,000 - 200,000
Population Doubling Time (h)
36 - 48
Expression
Cas9 (pLenti-EF1a-Cas9-Puro)
Warranty
abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Disclaimer
1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at licensing@abmgood.com.
2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Application
Research Use Only.
Material Citation
If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T3261
We do not perform extensive downstream characterization, or gene expression profiling of our cell lines. However, you can check the ‘References’ tab to see if a publication is available, or you can contact us to see if RNA or cell lysates are available for your cell line of interest. Please directly contact us for further information at quotes@abmgood.com.
We strongly encourage using the recommended media and culture conditions described in the "Growth Conditions" section as they have been optimized for cell growth. Other supplier's media and coating conditions have not been tested.
We strongly recommend thawing cells in the T25 flasks specified on the cell-specific product page to ensure optimal post-thaw recovery of the frozen cells before testing other culture vessels.
For a protocol on how to coat plates and dishes with Applied Cell Extracellular Matrix (Cat. No. G422), please download the “Applied Cell Extracellular Matrix Data Sheet” from here.
The standard concentration used for subculturing is 0.25% Trypsin-EDTA, available at abm (Cat. No. TM050). Yes, Trypsin-EDTA containing phenol red can be used as it does not interfere with trypsinization.
We follow the CDC-NIH recommendations that all mammalian sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products. This information can be found in 'Biosafety in Microbiological and Biomedical Laboratories' (1999). Your institution's Safety Officer or Technical Services will be able to make the call as to whether BioSafety Level I is possible with these cells at your site, if desired.
Cells that are properly frozen using an effective cryoprotective agent can be stored in liquid nitrogen vapor phase (or below -130°C) indefinitely without affecting viability.
Live cells: acclimatize for 3-5 hours at the recommended temperature and CO2 conditions stated for the cell line under the Growth Conditions section, and then change media afterwards. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
Frozen cells: Upon receipt, immediately place cells in liquid nitrogen; or below -130°C.
Live cells are shipped as a live culture in a T12.5 flask. Cells are seeded at an optimal confluency to avoid metabolic waste build up during transport. In addition, we thoroughly test our cell lines for functional freeze-thaw recovery. For more information on how to handle live cells, please view our detailed instructions here (https://www.abmgood.com/uploads/document/Cell_Handling_Instructions_Upon_Arrival_150623.pdf).
Cells form clumps and display attachment issues when the trypsinization agent is not effectively neutralized. To completely neutralize the trypsin agent, add an equal amount of complete growth media prior to centrifugation. Subtle changes in culture conditions, particularly in pH and the quality of serum used in the growth medium, may also affect the amount of clumping exhibited by the cells.
It is important to ensure cell clumps are broken up through gentle re-suspension by pipetting up and down several times after pelleting cells by centrifugation. This will ensure a homogenous single cell suspension for even plating.
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The process often takes months\u0026mdash;or even years\u0026mdash;making it both challenging and costly.\u003C\/p\u003E\n\u003Cp\u003ETo help researchers overcome these hurdles, \u003Cstrong\u003Eabm\u003C\/strong\u003E has undertaken an ambitious initiative to curate and develop a comprehensive collection of ready-to-use stable cell lines. Our mission is to reduce your costs and help you focus on what matters most\u0026mdash;your research.\u003C\/p\u003E\n\u003Cp\u003EWe have already assembled a library of over 1,000 stable cell lines, including more than 200 suspension cell lines, with new additions every week. Explore our extensive collection to find the perfect fit for your project. If you don\u0026rsquo;t see what you need, our expert scientists are ready to develop customized solutions tailored to your unique research requirements.\u003C\/p\u003E\n\u003C\/div\u003E\n\u003Cdiv class=\u0022abm-container-fluid\u0022\u003E\n\u003Cp class=\u0022abm-mt-5\u0022\u003E\u003Cb\u003ESearch our extensive collection using Gene name or Tissue type:\u003C\/b\u003E\u003C\/p\u003E\n\u003Cdiv id=\u0022outer-box\u0022\u003E\u003C!-- SEARCH SECTION --\u003E\n\u003Csection class=\u0022search-section\u0022\u003E\u003Cform action=\u0022\/search\u0022 class=\u0022ready-to-use-search-form\u0022\u003E\u003Cinput name=\u0022filter_id\u0022 readonly=\u0022readonly\u0022 type=\u0022hidden\u0022 value=\u002263\u0022 \/\u003E\n\u003Cdiv class=\u0022search-form-group\u0022 style=\u0022grid-template-columns: 10fr 2fr;\u0022\u003E\u003Cinput type=\u0022text\u0022 class=\u0022search-form-group-input\u0022 id=\u0022search-input\u0022 placeholder=\u0022Gene Name, Symbol or Accession Number\u0022 name=\u0022query\u0022 style=\u0022border-left: none; border-radius: 5px 0 0 5px;\u0022 \/\u003E\u003Cbutton class=\u0022search-form-group-button\u0022 type=\u0022button\u0022 id=\u0022search-button\u0022\u003E\u003Ci class=\u0022fa fa-search\u0022\u003E\u003C\/i\u003E\u003C\/button\u003E\u003C\/div\u003E\n\u003C\/form\u003E\u003C\/section\u003E\n\u003C!-- SEARCH RESULT SECTION --\u003E\n\u003Csection class=\u0022result-section\u0022\u003E\n\u003Cdiv id=\u0022result-list\u0022 class=\u0022abm-panel-list\u0022\u003E\u003C\/div\u003E\n\u003Cdiv id=\u0022paginate-section\u0022\u003E\u003C\/div\u003E\n\u003C\/section\u003E\n\u003C\/div\u003E\n\u003C\/div\u003E\n\u003Cstyle\u003E\n #outer-box {\n min-height: 300px;\n }\n\n #outer-box .loading {\n top: 150px;\n }\n\u003C\/style\u003E\n\u003Cp\u003E\n\u003Cscript\u003E\n window.addEventListener(\u0027load\u0027, () =\u003E {\n function getToken() {\n const el = document.head.querySelector(\u0027meta[name=\u0022X-CSRF-TOKEN\u0022]\u0027);\n if (el) {\n return el.getAttribute(\u0027content\u0027) || \u0027\u0027;\n }\n return \u0027\u0027;\n }\n\n function getProducts(page = 1) {\n const filter = \u002763\u0027;\n const query = document.getElementById(\u0027search-input\u0027).value;\n\n let loadingIndex = util.loading2(document.getElementById(\u0027outer-box\u0027));\n \/\/ Request data.\n fetch(\u0027\/product\/searchProducts\u0027, {\n method: \u0027POST\u0027,\n headers: {\n \u0027Content-Type\u0027: \u0027application\/json\u0027,\n },\n body: JSON.stringify({\n \u0027_token\u0027: getToken(),\n \u0027query\u0027: query,\n \u0027filter_id\u0027: filter,\n \u0027page\u0027: page,\n })\n }).then(response =\u003E response.json()).then(response =\u003E {\n if (response.code === 0 \u0026\u0026 response.data) {\n renderProducts(response.data.products);\n renderPaginate(response.data.page, response.data.lastPage);\n }\n util.closeLoading2(loadingIndex);\n });\n }\n\n function generateContentItem(key, value) {\n let html = \u0027\u0027;\n if (value) {\n html += \u0027\u003Cli\u003E\u0027;\n html += \u0027\u003Cspan class=\u0022attribute\u0022\u003E\u0027 + key + \u0027:\u003C\/span\u003E\u0027;\n html += \u0027\u003Cspan class=\u0022attribute-value\u0022\u003E\u0027 + value + \u0027\u003C\/span\u003E\u0027;\n html += \u0027\u003C\/li\u003E\u0027;\n }\n\n return html;\n }\n\n function renderProducts(products) {\n let html = \u0027\u0027;\n if (products \u0026\u0026 products.length) {\n html = \u0027\u003Cul style=\u0022padding-left:0;\u0022\u003E\u0027;\n for (const product of products) {\n let url = \u0027\u0027;\n let urlKey = \u0027\u0027;\n if (product.seo \u0026\u0026 product.seo.url_key) {\n urlKey = product.seo.url_key;\n if (\u0027\/\u0027 === urlKey[0]) {\n urlKey = urlKey.substr(1);\n }\n }\n\n if (urlKey) {\n url = \u0027\/\u0027 + urlKey;\n } else if (product.model_type) {\n const type = product.model_type.substr(22).toLowerCase();\n if (type) {\n url = \u0027\/catalog\/products\/\u0027 + product.id + \u0027\/type\/\u0027 + type;\n }\n }\n\n let price = parseFloat(product.price);\n if (price) {\n price = \u0027$\u0027 + price.toFixed(2);\n } else {\n price = \u0027Inquiry\u0027;\n }\n\n let productName = product._hl_name || product.name || \u0027\u0027;\n if (productName \u0026\u0026 product.filter_group_id \u0026\u0026 product.filter_group_id + \u0027\u0027 === \u00271\u0027 \u0026\u0026 product.species) {\n productName = productName.replace(new RegExp(\u0027^\\(.*?\\\\\\(\\)\\([^\\(\\)]*?\u0027 + product.species + \u0027.*?\\)\\(\\\\\\).*?\\)$\u0027), \u0027$1\u003Cspan class=\u0022species\u0022\u003E$2\u003C\/span\u003E$3\u0027);\n }\n\n html += \u0027\u003Cli class=\u0022abm-search-item\u0022\u003E\u0027;\n html += \u0027\u003Cdiv class=\u0022abm-search-item-container\u0022\u003E\u0027;\n html += \u0027\u003Cdiv class=\u0022abm-search-item-header\u0022\u003E\u0027; \/\/ abm-search-item-header-start\n html += \u0027\u003Cdiv class=\u0022abm-search-item-title\u0022\u003E\u0027; \/\/ abm-search-item-title-start\n html += \u0027\u003Cdiv class=\u0022abm-search-item-name\u0022\u003E\u0027; \/\/ abm-search-item-name-start\n html += \u0027\u003Ca class=\u0022abm-link\u0022 href=\u0022\u0027 + url + \u0027\u0022 title=\u0022\u0027 + product.name + \u0027\u0022\u003E\u0027;\n html += productName;\n html += \u0027\u003C\/a\u003E\u0027;\n html += \u0027\u003C\/div\u003E\u0027; \/\/ abm-search-item-name-end\n html += \u0027\u003C\/div\u003E\u0027; \/\/ abm-search-item-title-end\n html += \u0027\u003Cdiv class=\u0022abm-search-item-action\u0022\u003E\u0027; \/\/ abm-search-item-action-start\n html += \u0027\u003Ca class=\u0022btn abm-btn btn-block\u0022 href=\u0022\u0027 + url + \u0027\u0022 title=\u0022\u0027 + product.name + \u0027\u0022\u003EView Product\u003C\/a\u003E\u0027;\n html += \u0027\u003C\/div\u003E\u0027; \/\/ abm-search-item-action-end\n html += \u0027\u003C\/div\u003E\u0027; \/\/ abm-search-item-header-end\n html += \u0027\u003Chr class=\u0022abm-search-item-hr\u0022\u003E\u0027;\n html += \u0027\u003Cdiv class=\u0022abm-search-item-body\u0022\u003E\u0027; \/\/ abm-search-item-body-start\n html += \u0027\u003Cdiv class=\u0022abm-search-item-content\u0022\u003E\u0027; \/\/ abm-search-item-content-start\n\n html += \u0027\u003Cul class=\u0022abm-search-item-content-base\u0022\u003E\u0027; \/\/ abm-search-item-content-base-start\n html += generateContentItem(\u0027Cat. No.\u0027, product._hl_cat_no || product.cat_no || \u0027\u0027);\n html += generateContentItem(\u0027Price\u0027, price || \u0027Inquiry\u0027);\n html += generateContentItem(\u0027Unit\u0027, product.unit_quantity || \u0027\u0027);\n html += \u0027\u003C\/ul\u003E\u0027; \/\/ abm-search-item-content-base-end\n html += \u0027\u003Cul class=\u0022abm-search-item-content-info\u0022\u003E\u0027; \/\/ abm-search-item-content-info-start\n html += generateContentItem(\u0027Accession number\u0027, product._hl_accession_number || product.accession_number || \u0027\u0027);\n html += generateContentItem(\u0027Gene name\u0027, product._hl_gene_name || product.gene_name || \u0027\u0027);\n html += generateContentItem(\u0027Other aliases\u0027, product._hl_alias || product.alias || \u0027\u0027);\n html += generateContentItem(\u0027Species\u0027, product.species || \u0027\u0027);\n html += generateContentItem(\u0027CDS size\u0027, product.gene \u0026\u0026 product.gene.cds ? 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Offering custom solutions for every gene in the human, mouse, and rat genome.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":"abm_catalog_cellular","image":null,"independentPage":0,"top_type":1,"sort_order":202,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-02-06 07:23:47"},"info":{"id":5218,"cat_no_base":"T3261","parent_id":38141,"image":null,"price-original":null,"description":"\u003Cp\u003EThis cell line stably expresses spCas9 Nuclease.\u0026nbsp;\u003C\/p\u003E","shipment_type":null,"internal_leadtime":"0","lysate_type":null,"small_image":null,"growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003EThis cell line stably expresses spCas9 Nuclease.\u0026nbsp;\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"RPMI + 10% FBS + 1% P\/S + 0.4 \u00b5g\/ml puromycin","alias":null,"tier_price":null,"operator_no_use":"ryan","addtime":"2016-12-19 00:00:00","cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":null,"expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":"0","cell_morphology_specification":null,"tax_class_id":2,"inventory_location":"D4R4B4 (D3-E2); New G6 (E4-E7)","vector":null,"internal_note":"\u003Cp\u003EpLenti-EF1a-Cas9-Puro\u003C\/p\u003E\n\u003Cp\u003ER4 clone; expression QC by WB\u003C\/p\u003E\n\u003Cp\u003E************************************** Lot Passage No. *************************************\u003C\/p\u003E\n\u003Cp\u003EC23M25AA: P=U+3 |\u0026nbsp;C23S22TA: P=10\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":null,"seeding_density_ml":"100,000 - 200,000","source":null,"source_catno":"\u003Cp\u003E640\/2495\u003C\/p\u003E","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":"36 - 48","source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":"ABM","donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":"2","biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003ESuspension, round\u003C\/p\u003E","expression_profile":null,"storage":null,"propagation_method":"\u003Cp\u003EPriGrow II (TM002) + 10% FBS + 1% Penicillin\/Streptomycin Solution (G255), 37.0\u0026deg;C, 5% CO\u2082. 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If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and procedures. The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period.\u0022\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":"ABM","internal_product_note":null,"donor_history":"Male, 14, T cell leukemia","split_ratio":null,"expression":"\u003Cp\u003ECas9 (pLenti-EF1a-Cas9-Puro)\u003C\/p\u003E","growth_conditions":"\u003Cp\u003E\u003Cstrong style=\u0022color: rgb(255, 0, 0); text-wrap: wrap;\u0022\u003E\u003Cstrong\u003EPriCoat\u2122 T25 Flasks (\u003Ca href=\u0022https:\/\/www.abmgood.com\/pricoat-t25-flasks.html\u0022 target=\u0022_self\u0022\u003EG299\u003C\/a\u003E) are recommended for optimal cell culture.\u003C\/strong\u003E\u0026nbsp;\u003C\/strong\u003E RPMI 1640 Medium (\u003Ca href=\u0022https:\/\/www.abmgood.com\/RPMI-1640-Medium.html\u0022 target=\u0022_self\u0022\u003ETM503\u003C\/a\u003E) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (\u003Ca href=\u0022https:\/\/www.abmgood.com\/penicillin-streptomycin-solution-g255.html\u0022 target=\u0022_self\u0022 style=\u0022text-wrap: wrap;\u0022\u003EG255\u003C\/a\u003E), 37.0\u00b0C, 5% CO\u2082.\u0026nbsp;\u003C\/p\u003E\u003Cbr\u003E\u003C\/br\u003E\u003Cp\u003E0.4 \u00b5g\/ml Puromycin (\u003Ca href=\u0022https:\/\/www.abmgood.com\/puromycin-g264.html\u0022 target=\u0022_self\u0022\u003EG264\u003C\/a\u003E) for selection. \u003Cbr\u003E\u003C\/br\u003E\nNote: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions. \u003C\/p\u003E\u003Cp\u003E\u003Ci\u003E*Do not heat-inactivate\u003C\/i\u003E\u003C\/p\u003E","recommend":"\u003Cp\u003EWorried about losing your cells due to growth or thawing difficulties, or even a random freezer breakdown? Enjoy peace of mind knowing that you can be covered under\u00a0\u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s\u00a0\u003Ca class=\u0022abm-link\u0022 href=\u0022\/cell-line-insurance.html\u0022\u003ECell Line Insurance\u003C\/a\u003E.\u003C\/p\u003E\r\n\u003Cp\u003ESale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing institution.\u003C\/p\u003E\r\n\u003Cp\u003EFor for-profit organizations, please contact \u003Ca class=\u0022abm-link\u0022 href=\u0022mailto:quotes@abmgood.com\u0022\u003Equotes@abmgood.com\u003C\/a\u003E for pricing.\u003C\/p\u003E ","references":null,"cryopreservation":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003ECryopreservation Medium (\u003C\/span\u003E\u003Ca style=\u0022text-wrap: wrap;\u0022 href=\u0022https:\/\/www.abmgood.com\/cryopreservation-medium-tm024.html\u0022 target=\u0022_self\u0022\u003ETM024\u003C\/a\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E), or complete growth media with 10% DMSO.\u003C\/span\u003E\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","promotions":null,"sync_images":null,"thawing_protocol":"\u003Cp\u003E1. Thaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-15 06:28:27","updated_at":"2025-03-29 00:45:38","concentration":null},"seo":{"id":67007,"catalog_id":38141,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"cas9-expressing-jurkat-cell-line.html","meta_title":"Cas9 Expressing Jurkat Cell Line","meta_keywords":"cas9 expressing jurkat cell line, jurkat cells expresses cas9","meta_description":"The Cas9 Expressing Jurkat Cell Line stably expresses spCas9 Nuclease and shows superconductivity at room temperature and ambient pressure.","created_at":"2022-09-23 06:53:13","updated_at":"2024-08-30 20:23:10"},"maps":[],"media":[{"id":388782,"parent_id":38141,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/fRiwOT06fFvocqyTFkuQcfK615XiQJBdV3FANWkc.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-29 00:45:17","updated_at":"2025-03-29 00:45:17"},{"id":388783,"parent_id":38141,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/upload\/6r1ullQ6ajhfVb51fW3DKOJ5BXK3srBBmXjWDsEe.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":null,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2025-03-29 00:45:24","updated_at":"2025-03-29 00:45:24"}],"gene":null}