Cas9 Nickase Adenovirus

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Due to the requirement for two sgRNA guide sequences to be close together for gene editing to occur, using Cas9 nickase should cause fewer off-target effects than using Cas9 nuclease.\u003C\/p\u003E","disclaimer":"\u003Ca href=\u0022\/terms#disclaimer-section\u0022\u003EClick for more info\u003C\/a\u003E","application":null,"components":null,"cas9_origin":null,"concentration":null,"enzymes_size":null,"genecraft_series":null,"guarantee":"","population":null,"qc":null,"format_general":null,"including_screening_kit":null,"expression_system_general":null,"purity":null,"image":null,"insert_size":null,"shipping_conditions":"\u003Cp\u003EHigh titer adenoviruses are shipped with dry ice and low titer adenoviruses are shipped with ice packs. For long term storage, it is recommended to store the viruses at -80\u0026deg;C in small aliquots to avoid repeated freeze-thaw cycles.\u003C\/p\u003E","source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":"pAdeno-Cas9-Nickase","reaction_buffer":null,"storage_buffer":null,"caution":"This product is for research use only and is not intended for therapeutic or diagnostic applications. 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18:54:29","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[{"id":327,"name":"pAdeno-Cas9-Nickase","sequence":"ttaattaacgatttcgaacccggggtaccgaattcctcgagtctagaggagcatgcgacgtcgcaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcacgtaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgcctatttcacaccgcatacaggtggcacttttcggcgaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaanaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtcggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcccgggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtacctcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatggcggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtcacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactgccgaactacttactctagcttcccggcaacaattaatagactggatggaggcgcataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgcgtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcgctggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatcagaaagcgccacgcttcccgaaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcggttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcccattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctatttaggtgacggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcggttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcccattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctatttaggtgacactatagaatactcaagctagttaattaacgttaattaacatcatcaataatataccttattttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgtgggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaacacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacaggaagtgacaattttcgcgccgttttaggcggatgttgtagtaaatttgggcgtaaccgagtaagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttgtgttactcatagcgcgtaatctctagcatcgtaactataaccgtcctaaggtagcgaagctcagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagagctagcgtttaaacttaagcttGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGATAAGAAATACTCAATAGGACTGGCTATTGGCACAAATAGCGTCGGATGGGCTGTGATCACTGATGAATATAAGGTTCCTTCTAAAAAGTTCAAGGTTCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGGGCTCTTCTGTTTGACAGTGGAGAGACAGCCGAAGCTACTAGACTCAAACGGACAGCTAGGAGAAGGTATACAAGACGGAAGAATAGGATTTGTTATCTCCAGGAGATTTTTTCAAATGAGATGGCCAAAGTGGATGATAGTTTCTTTCATAGACTTGAAGAGTCTTTTTTGGTGGAAGAAGACAAGAAGCATGAAAGACATCCTATTTTTGGAAATATAGTGGATGAAGTTGCTTATCACGAGAAATATCCAACTATCTATCATCTGAGAAAAAAATTGGTGGATTCTACTGATAAAGCCGATTTGCGCCTGATCTATTTGGCCCTGGCCCACATGATTAAGTTTAGAGGTCATTTTTTGATTGAGGGCGATCTGAATCCTGATAATAGTGATGTGGACAAACTGTTTATCCAGTTGGTGCAAACCTACAATCAACTGTTTGAAGAAAACCCTATTAACGCAAGTGGAGTGGATGCTAAAGCCATTCTTTCTGCAAGATTGAGTAAATCAAGAAGACTGGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCCTGTTTGGGAATCTCATTGCTTTGTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATGCTAAACTCCAGCTTTCAAAAGATACTTACGATGATGATCTGGATAATCTGTTGGCTCAAATTGGAGATCAATATGCTGATTTGTTTTTGGCAGCTAAGAATCTGTCAGATGCTATTCTGCTTTCAGACATCCTGAGAGTGAATACTGAAATAACTAAGGCTCCCCTGTCAGCTTCAATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTCTGAAAGCCCTGGTTAGACAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCAGGTTATATTGATGGCGGCGCAAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTCTGGAAAAAATGGATGGTACTGAGGAACTGTTGGTGAAACTGAATAGAGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAACGGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAGACAAGAAGACTTTTATCCATTTCTGAAAGACAATAGAGAGAAGATTGAAAAAATCTTGACTTTTAGGATTCCTTATTATGTTGGTCCATTGGCCAGAGGCAATAGTAGGTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAATTTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAAAATCTTCCAAATGAAAAAGTGCTGCCAAAACATAGTTTGCTTTATGAGTATTTTACCGTTTATAACGAATTGACAAAGGTCAAATATGTTACTGAAGGAATGAGAAAACCAGCATTTCTTTCAGGTGAACAGAAGAAAGCCATTGTTGATCTGCTCTTCAAAACAAATAGGAAAGTGACCGTTAAGCAACTGAAAGAAGATTATTTCAAAAAAATAGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAATGCTTCACTGGGTACATACCATGATTTGCTGAAAATTATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGACATCCTGGAGGATATTGTTCTGACATTGACCCTGTTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATACGCTCACCTCTTTGATGATAAGGTGATGAAACAGCTTAAAAGACGCAGATATACTGGTTGGGGAAGGTTGTCCAGAAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATACTGGATTTTTTGAAATCAGATGGTTTTGCCAATCGCAATTTTATGCAGCTCATCCATGATGATAGTTTGACATTTAAAGAAGACATCCAAAAAGCACAAGTGTCTGGACAAGGCGATAGTCTGCATGAACATATTGCAAATCTGGCTGGTAGCCCTGCTATTAAAAAAGGTATTCTCCAGACTGTGAAAGTTGTTGATGAATTGGTCAAAGTGATGGGGCGGCATAAGCCAGAAAATATCGTTATTGAAATGGCAAGAGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCCAGAGAGAGGATGAAAAGAATCGAAGAAGGTATCAAAGAACTGGGAAGTCAGATTCTTAAAGAGCATCCTGTTGAAAATACTCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGAGATATGTATGTGGACCAAGAACTGGATATTAATAGGCTGAGTGATTATGATGTCGATCACATTGTTCCACAAAGTTTCCTTAAAGACGATTCAATAGACAATAAGGTCCTGACCAGGTCTGATAAAAATAGAGGTAAATCCGATAACGTTCCAAGTGAAGAAGTGGTCAAAAAGATGAAAAACTATTGGAGACAACTTCTGAACGCCAAGCTGATCACTCAAAGGAAGTTTGATAATCTGACCAAAGCTGAAAGAGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAATTGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAATACTAAATACGATGAAAATGATAAACTTATTAGAGAGGTTAAAGTGATTACCCTGAAATCTAAACTGGTTTCTGACTTCAGAAAAGATTTCCAATTCTATAAAGTGAGAGAGATTAACAATTACCATCATGCCCATGATGCCTATCTGAATGCCGTCGTTGGAACTGCTTTGATTAAGAAATATCCAAAACTTGAAAGCGAGTTTGTCTATGGTGATTATAAAGTTTATGATGTTAGGAAAATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAGTATTTCTTTTACTCTAATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCCCTCTGATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGAGAGATTTTGCCACAGTGCGCAAAGTGTTGTCCATGCCCCAAGTCAATATCGTCAAGAAAACAGAAGTGCAGACAGGCGGATTCTCTAAGGAGTCAATTCTGCCAAAAAGAAATTCCGACAAGCTGATTGCTAGGAAAAAAGACTGGGACCCAAAAAAATATGGTGGTTTTGATAGTCCAACCGTGGCTTATTCAGTCCTGGTGGTTGCTAAGGTGGAAAAAGGGAAATCCAAGAAGCTGAAATCCGTTAAAGAGCTGCTGGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAATCCCATTGACTTTCTGGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACCTGATCATTAAACTGCCTAAATATAGTCTTTTTGAGCTGGAAAACGGTAGGAAACGGATGCTGGCTAGTGCCGGAGAACTGCAAAAAGGAAATGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTCTGTATCTGGCTAGTCATTATGAAAAGTTGAAGGGTAGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAGCAGCATAAGCATTATCTGGATGAGATTATTGAGCAAATCAGTGAATTTTCTAAGAGAGTTATTCTGGCAGATGCCAATCTGGATAAAGTTCTTAGTGCATATAACAAACATAGAGACAAACCAATAAGAGAACAAGCAGAAAATATCATTCATCTGTTTACCTTGACCAATCTTGGAGCACCCGCTGCTTTTAAATACTTTGATACAACAATTGATAGGAAAAGATATACCTCTACAAAAGAAGTTCTGGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATTGATTTGAGTCAGCTGGGAGGTGACCCCAAGAAAAAACGCAAGGTGGAAGATCCTAAGAAAAAGCGGAAAGTGGACACGCGTACGCGGCCGCTCGAGtctagagggcccgtttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctctatgtcgggtgcatctatctcgggtccggagaaagaggtaatgaaatggcatgggcgtggcttaagggtgggaaagaatatataaggtgggggtcttatgtagttttgtatctgttttgcagcagccgccgccgccatgagcaccaactcgtttgatggaagcattgtgagctcatatttgacaacgcgcatgcccccatgggccggggtgcgtcagaatgtgatgggctccagcattgatggtcgccccgtcctgcccgcaaactctactaccttgacctacgagaccgtgtctggaacgccgttggagactgcagcctccgccgccgcttcagccgctgcagccaccgcccgcgggattgtgactgactttgctttcctgagcccgcttgcaagcagtgcagcttcccgttcatccgcccgcgatgacaagttgacggctcttttggcacaattggattctttgacccgggaacttaatgtcgtttctcagcagctgttggatctgcgccagcaggtttctgccctgaaggcttcctcccctcccaatgcggtttaaaacataaataaaaaaccagactctgtttggatttggatcaagcaagtgtcttgctgtctttatttaggggttttgcgcgcgcggtaggcccgggaccagcggtctcggtcgttgagggtcctgtgtattttttccaggacgtggtaaaggtgactctggatgttcagatacatgggcataagcccgtctctggggtggaggtagcaccactgcagagct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