Cas9 Nickase H840A NLS Protein

{"id":1934,"cat_no":"K138","sku_in_m2":"","old_cat_no":"K138","name":"Cas9 Nickase H840A NLS Protein","category_id":208,"category_name":"Cas Proteins \u0026 CRISPR Screening","frontend_category_id":71,"frontend_category_ids":"86,57,67,71","gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"40\u00b5g (250pmol\/ 25\u00b5L)","accession_number":"","species_id":0,"species":"","raised_in_id":0,"raised_in":"","cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","price":"142.0000","price_before_discount":"0.0000","keywords":"cas9 proteins","invisible_search_keywords":"Cas9 Enzymes, Cas9 Enzyme, Cas9 protein, cas9 proteins","filter_group_id":34,"filter_group_name":"Molecular Biology","filter_id":41,"filter_name":"Kits \u0026 Other Enzymes","search_filter_ids":"32,34","search_filter_keys":"32-34","temp_field":null,"customization":0,"filter_table_id":18085070,"filter_table_name":"","related_group_id":17,"url":"","operator":"","points_rule":7,"status":1,"need_remove":0,"document_sort":"","price_old":"135.0000","price2024":"142.0000","created_at":null,"updated_at":"2025-01-06 08:30:36","Confirmed":"Need Confirm","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"cas9-proteins.html","frontendBreadcrumbs":[{"id":57,"pid":86,"name":"Genetic Materials","page_id":8,"page_type":"App\\Models\\Category","href":null,"sort":57,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":"2025-01-20 08:46:24","url_key":"genetic-materials.html","url":"genetic-materials.html"},{"id":67,"pid":57,"name":"CRISPR","page_id":15,"page_type":"App\\Models\\Category","href":null,"sort":67,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"CRISPR-Cas9-sgRNA.html","url":"CRISPR-Cas9-sgRNA.html"},{"id":71,"pid":67,"name":"Cas Proteins \u0026 CRISPR Screening","page_id":223,"page_type":"App\\Models\\Cms","href":null,"sort":71,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"cas9-proteins.html","url":"cas9-proteins.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseMolecular","geo_price":"142.00","geo_price_symbol":"$142.00","category":{"id":208,"categories":"Cas Proteins \u0026 CRISPR Screening","pid":15,"url_key":"cas9-proteins.html","css":null,"wid":0,"breadcrumbs":null,"breadcrumbsIdJson":"","breadcrumbsHtml":"","description":"\u003Cp\u003ECas9 proteins\u003C\/p\u003E\n\u003Cdiv id=\u0022gtx-trans\u0022 style=\u0022position: absolute; left: -35px; top: -10.5px;\u0022\u003E\n\u003Cdiv class=\u0022gtx-trans-icon\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Cas Proteins \u0026 CRISPR Screening","meta_keywords":"Cas Proteins \u0026 CRISPR Screening","meta_description":"Cas Proteins \u0026 CRISPR Screening","deleted_at":null,"enable":"N","parent_list":"8,15","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":"2024-08-28 23:24:53","updated_at":"2024-12-19 17:58:36"},"info":{"id":17156,"cat_no_base":"K138","parent_id":1934,"description":"\u003Cp\u003EThe Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)\/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease. \u003Cbr \/\u003E\u003Cbr \/\u003ETo improve the off-target mutagenic effects of this system, the Cas9 Nickase H840A Protein was developed with a H840A mutation in its HNH-like nuclease domain. Unlike the Cas9 nuclease, this mutant form generates a single-stranded nick instead of a double-strand break (DSB). Because a single DNA nick is quickly repaired with high fidelity by the cellular machinery, the system requires two closely juxtaposed nicks in order to trigger the same genomic disruption as the Cas9 nuclease. This effectively boosts the recognition sequence to 40 instead of 20 nucleotides, and, as a result, off-target effects become highly unlikely. Thus, the double-nickase CRISPR system offers unparalleled specificity to satisfy even the most stringent of experimental requirements.\u003Cbr \/\u003E\u003Cbr \/\u003EThe Cas9 nuclease from the bacteria \u003Cem\u003EStreptococcus pyogenes\u003C\/em\u003E, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First, the spCas9 PAM sequence is 5\u0026rsquo;-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency \u003Cem\u003Ein vivo \u003C\/em\u003Ecompared to other variants. Cas9 H840 Nickase NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First, the spCas9 PAM sequence is 5\u0026rsquo;-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency \u003Cem\u003Ein vivo \u003C\/em\u003Ecompared to other variants. Cas9 H840 Nickase NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; width: 100%; height: 50px; background-color: #f9eae4;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022height: 89px;\u0022 valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022width: 91.0861%; height: 59px;\u0022\u003E\n\u003Cp style=\u0022text-align: center;\u0022\u003E\u003Cspan style=\u0022font-size: 14pt;\u0022\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a variety of Cas9 proteins. View our whole collection \u003Ca href=\u0022\/cas9-proteins.html\u0022\u003Ehere\u003C\/a\u003E.\u003C\/span\u003E\u003C\/p\u003E\n\u003C\/td\u003E\n\u003C\/tr\u003E\n\u003C\/tbody\u003E\n\u003C\/table\u003E\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","disclaimer":null,"application":null,"components":null,"cas9_origin":"209","concentration":"10 \u00b5M, 1.60 mg\/ml","enzymes_size":null,"genecraft_series":null,"guarantee":null,"population":null,"qc":null,"format_general":"Enzyme supplied with 10X Reaction Buffer","including_screening_kit":null,"expression_system_general":null,"purity":null,"image":"\/k\/0\/k038-k138.jpg","insert_size":null,"shipping_conditions":null,"source_catalog_number":null,"inactivation_protocol":null,"led_viewer_compatibility":null,"unit_definition":null,"vector":null,"reaction_buffer":null,"storage_buffer":"10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v\/v) Glycerol.","caution":"This product is distributed for laboratory research only. Not for diagnostic use.","storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"Extra Freezer 1 door","note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":"Nickase","cas_origin":"spCas9","cas_protein_marker":"No GFP","source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-22 08:56:44","updated_at":"2024-02-29 23:01:00","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379799,"parent_id":1934,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/c\/a\/cas9-nickase_h840a_v3.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18085070,"sku_in_m2":"K138","value_id_m2":49909715,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:39:28"},{"id":385030,"parent_id":1934,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/uu4IP7lal9a8yJRefMjC7KJPrAqU2gLM06hAf2bp.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-11-18 21:20:14","updated_at":"2024-11-18 21:20:19"},{"id":379800,"parent_id":1934,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/k\/0\/k038-k138.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":0,"entity_id_m2":18085070,"sku_in_m2":"K138","value_id_m2":49909747,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2024-11-18 21:20:19"}],"gene":null}