Cas9 Nuclease Protein

Cat. No.
K108
Unit
40µg (250pmol/ 25µL)
Price
$84.00
Cat. No. K108
Name Cas9 Nuclease Protein
Unit 40µg (250pmol/ 25µL)
Category Cas Proteins & CRISPR Screening
Description

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).

The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.

abm offers a variety of Cas9 proteins. View our whole collection here.

 

Cas Type Nuclease
Cas Origin spCas9
Cas Protein Marker No GFP
Concentration 10 µM, 1.60 mg/ml
Format General Enzyme supplied with 10X Reaction Buffer
Storage Buffer 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Caution This product is distributed for laboratory research only. Not for diagnostic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K108
Search CoA here
  • Bláhová, Z., Franěk, R., Let, M., Bláha, M., Pšenička, M., & Mráz, J. (2022). Partial fads2 Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Δ8 Pathway with an Impact on the Reproduction of Female Zebrafish (Danio rerio). Genes, 13(4), 700. https://doi.org/10.3390/genes13040700
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