Cas9 Null Mutant GFP NLS Protein (High Concentration)

{"id":1943,"cat_no":"K186","sku_in_m2":"","old_cat_no":"K186","name":"Cas9 Null Mutant GFP NLS Protein (High Concentration)","category_id":208,"category_name":"Cas Proteins \u0026 CRISPR Screening","frontend_category_id":71,"frontend_category_ids":"86,57,67,71","gene_id":0,"gene_name":"","gene_full_name":"","alias":"","unit_quantity":"47\u00b5g (250pmol\/ 25\u00b5L)","accession_number":"","species_id":0,"species":"","raised_in_id":0,"raised_in":"","cell_type_id":0,"cell_type":"","tissue_id":0,"tissue":"","price":"162.0000","price_before_discount":"0.0000","keywords":"cas9 proteins, dCas9, Cas9d, spCas9d, Dead Cas9, Cas9 Double Mutant, Nuclease-deficient Cas9, CRISPR-associated endonuclease dCas9 from \u003Ci\u003EStreptococcus pyogenes\u003C\/i\u003E","invisible_search_keywords":"cas9nullmutant, Cas9 Enzymes, Cas9 Enzyme, Cas9 protein, cas9 proteins","filter_group_id":34,"filter_group_name":"Molecular Biology","filter_id":41,"filter_name":"Kits \u0026 Other Enzymes","search_filter_ids":"32,34","search_filter_keys":"32-34","temp_field":null,"customization":0,"filter_table_id":18085079,"filter_table_name":"","related_group_id":17,"url":"","operator":"","points_rule":7,"status":1,"need_remove":0,"document_sort":"","price_old":"155.0000","price2024":"162.0000","created_at":null,"updated_at":"2025-01-06 08:30:48","Confirmed":"Need Confirm","geo_price_before_discount":"0.00","geo_titer7_price_before_discount":"149.00","cate_url":"cas9-proteins.html","frontendBreadcrumbs":[{"id":57,"pid":86,"name":"Genetic Materials","page_id":8,"page_type":"App\\Models\\Category","href":null,"sort":57,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":"2025-01-20 08:46:24","url_key":"genetic-materials.html","url":"genetic-materials.html"},{"id":67,"pid":57,"name":"CRISPR","page_id":15,"page_type":"App\\Models\\Category","href":null,"sort":67,"show_in_nav":1,"show_in_homepage":1,"has_children":1,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"CRISPR-Cas9-sgRNA.html","url":"CRISPR-Cas9-sgRNA.html"},{"id":71,"pid":67,"name":"Cas Proteins \u0026 CRISPR Screening","page_id":223,"page_type":"App\\Models\\Cms","href":null,"sort":71,"show_in_nav":1,"show_in_homepage":1,"has_children":0,"status":1,"related_table":null,"created_at":null,"updated_at":null,"url_key":"cas9-proteins.html","url":"cas9-proteins.html"}],"price_flag":0,"builderURL":"","builderViewerURL":"","mapURL":"","viewerURL":"","builderType":"","productType":"App\\Models\\CatalogBaseMolecular","geo_price":"162.00","geo_price_symbol":"$162.00","category":{"id":208,"categories":"Cas Proteins \u0026 CRISPR Screening","pid":15,"url_key":"cas9-proteins.html","css":null,"wid":0,"breadcrumbs":null,"breadcrumbsIdJson":"","breadcrumbsHtml":"","description":"\u003Cp\u003ECas9 proteins\u003C\/p\u003E\n\u003Cdiv id=\u0022gtx-trans\u0022 style=\u0022position: absolute; left: -35px; top: -10.5px;\u0022\u003E\n\u003Cdiv class=\u0022gtx-trans-icon\u0022\u003E\u003C\/div\u003E\n\u003C\/div\u003E","meta_title":"Cas Proteins \u0026 CRISPR Screening","meta_keywords":"Cas Proteins \u0026 CRISPR Screening","meta_description":"Cas Proteins \u0026 CRISPR Screening","deleted_at":null,"enable":"N","parent_list":"8,15","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":null,"in_footer":1,"fid":null,"created_at":"2024-08-28 23:24:53","updated_at":"2024-12-19 17:58:36"},"info":{"id":17169,"cat_no_base":"K186","parent_id":1943,"description":"\u003Cp\u003EThe Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)\/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. \u003Cbr \/\u003EThe Cas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type Cas9 (D10A and H840A). Such a Cas9 protein retains its ability to bind to genomic DNA through gRNA:genomic DNA base pairing, however, unlike Cas9 Nuclease and Cas9 Nickase, where permanent gene disruption can be achieved, the Cas9 Null Mutant does not introduce any genome modifications. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression.\u003C\/p\u003E\n\u003Cp\u003E\u003Cbr \/\u003EThe Cas9 from the bacteria \u003Cem\u003EStreptococcus pyogenes\u003C\/em\u003E, abbreviated spCas9, is the most commonly used Cas9 variant.The fusion of Cas9 to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Null Mutant GFP can also be used for FACS applications and screening. Cas9 D10A Null Mutant GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.\u003C\/p\u003E\n\u003Ctable style=\u0022border-collapse: collapse; width: 100%; height: 50px; background-color: #f9eae4;\u0022 border=\u00220\u0022 cellpadding=\u002210\u0022\u003E\n\u003Ctbody\u003E\n\u003Ctr style=\u0022height: 89px;\u0022 valign=\u0022middle\u0022\u003E\n\u003Ctd style=\u0022width: 91.0861%; height: 59px;\u0022\u003E\n\u003Cp style=\u0022text-align: center;\u0022\u003E\u003Cspan style=\u0022font-size: 14pt;\u0022\u003E\u003Cstrong\u003Eabm\u003C\/strong\u003E offers a variety of Cas9 proteins. 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Not for diagnostic use.","storage_condition":"Store all components at -20\u00b0C.","product_volume":null,"reporter":null,"safeview_series":null,"source_catno":null,"stain_color":null,"supplier":"ABM","internal_supplier":null,"internal_note":null,"inventory_location":"Extra Freezer 1 door","note":null,"recommend":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"royalty_rates":null,"cas_type":"Null Mutant","cas_origin":"spCas9","cas_protein_marker":"GFP","source":null,"endotoxin_level":null,"additional_information":null,"titer":null,"nucleotide_format":null,"protocol_overview":null,"source_price":null,"created_at":"2022-09-22 08:56:45","updated_at":"2024-02-29 23:02:19","short_description":null,"reaction_definition":null,"specificity":null,"promotions":null},"maps":[],"media":[{"id":379810,"parent_id":1943,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/c\/a\/cas9-null-mutant-gfp.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18085079,"sku_in_m2":"K186","value_id_m2":49909703,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2023-04-10 05:39:28"},{"id":385032,"parent_id":1943,"parent_type":"App\\Models\\CatalogBaseMolecular","file_path":"\/upload\/xZm45X0OTht5GFyg9QiA2H8U2n4RUiQY9yKJ0kL2.png","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":1,"entity_id_m2":null,"sku_in_m2":null,"value_id_m2":null,"attribute_id":0,"created_at":"2024-11-18 21:21:37","updated_at":"2024-11-18 21:21:41"}],"gene":null}