circRNA Isolation Kit
Cat. No. | G4001 |
Name | circRNA Isolation Kit |
Unit | 25 Reactions |
Description |
abm’s circRNA Isolation Kit allows for the enrichment of a novel class of RNA called circular RNA from a total RNA sample. Poly(A) Polymerase, E. coli catalyzes the addition of adenosine residues to the 3’ termini of all linear RNAs – including structured RNAs. This results in RNAs exhibiting a long, 3’ unstructured overhang which is optimally bound by RNase R. RNase R can then illicit 3’ to 5’ exonuclease activity resulting in improved degradation of linear RNAs which contributes to higher purity circRNAs. |
Application |
Enhanced circRNA isolation and enrichment. |
Storage Condition |
•Store all components except RNA Purification Magnetic Beads at -20°C •Store RNA Purification Magnetic Beads at 4°C |
Note |
A magnetic separation rack is required (not included) |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G4001 |
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