dCas9-DNMT3A Lentiviral Vector

Cat. No.
K091
Unit
10 µg
Price
$212.00
Cat. No. K091
Name dCas9-DNMT3A Lentiviral Vector
Unit 10 µg
Description

This construct consists of the catalytic domain of DNA methyltransferase (DNMT3A) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities. The DNMT3A domain facilitates CpG site methylation leading to transcriptional down-regulation. By using specific sgRNAs, the dCas9-DNMT3A can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest.

Vector pLenti-EF1a-dCas9-DNMT3A
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K091
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