dCas9-TET1CD Lentiviral Vector
Cat. No. | K096 |
Name | dCas9-TET1CD Lentiviral Vector |
Unit | 10 µg |
Description |
This construct consists of the catalytic domain of Ten-Eleven Translocation dioxygenase 1 (TET1CD) fused to the C-terminus of Cas9 Double Mutant (dCas9). The Cas9 Double Mutant has changes at amino acid positions D10A and H840A which completely inactivate both nuclease and nickase activities; whereas the TET1 domain facilitates the process of demethylation leading to transcriptional up-regulation. By using specific sgRNAs, the dCas9-TET1CD can be targeted to a promoter region allowing for epigenetic control over the expression of almost any gene of interest. |
Vector | pLenti-EF1a-dCas9-tet1CD |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K096 |
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