Enhanced Primary Human Liver Sinusoidal Endothelial Cells

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No.:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.cat_no}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EPrice:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${price}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003Cli\u003E\n \u003Cspan class=\u0022attribute\u0022\u003EUnit:\u003C\/span\u003E\n \u003Cspan class=\u0022attribute-value\u0022\u003E${product.unit_quantity}\u003C\/span\u003E\n \u003C\/li\u003E\n \u003C\/ul\u003E\n \u003C\/div\u003E\n ${mediaPart}\n \u003C\/div\u003E\n \u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n document.querySelector(\u0027#result-container\u0027).innerHTML = html;\n }\n\n function getProductUrl(product) {\n if (product.seo \u0026\u0026 product.seo.url_key) {\n return \u0027\/\u0027 + ltrim(product.seo.url_key, \u0027\/\u0027);\n }\n\n const type = product.model_type.substr(22).toLowerCase();\n return \u0027\/catalog\/products\/\u0027 + product.id + \u0027\/type\/\u0027 + type;\n }\n\n function getProductPrice(product) {\n return isNaN(Number(product.geo_price)) ? (product.geo_price || (\u0027$\u0027 + (Number(product.price) || 0).toFixed(2))) : (\u0027$\u0027 + (Number(product.geo_price) || 0).toFixed(2));\n }\n\n function generateMediaPart(product) {\n let html = \u0027\u0027;\n if (product.media \u0026\u0026 product.media.length) {\n html += \u0027\u003Cdiv class=\u0022abm-search-item-media\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel slide\u0022 data-ride=\u0022carousel\u0022\u003E\u0027\n + \u0027\u003Cdiv class=\u0022carousel-inner\u0022 role=\u0022listbox\u0022\u003E\u0027\n\n let active = true;\n for (const media of product.media) {\n html += `\n \u003Cdiv class=\u0022item ${active ? \u0027active\u0027 : \u0027\u0027}\u0022\u003E\n \u003Cimg src=\u0022\/assets\/product\/${media.file_path}\u0022 alt=\u0022\u0022\u003E\n \u003C\/div\u003E\n `;\n active = false;\n }\n html += \u0027\u003C\/div\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027;\n }\n\n return html;\n }\n\n function generateCategoryPart(product) {\n return \u0027\u0027;\n let html = \u0027\u0027;\n if (product.category) {\n html += `\n \u003Cdiv class=\u0022abm-search-item-item-type\u0022\u003E\n \u003Ca href=\u0022${product.category.url_key}\u0022\u003E${product.category.categories}\u003C\/a\u003E\n \u003C\/div\u003E\n `;\n }\n return html;\n }\n\n function ltrim(str, charlist) {\n if (charlist === undefined)\n charlist = \u0022\\s\u0022;\n return str.replace(new RegExp(\u0022^[\u0022 + charlist + \u0022]+\u0022), \u0022\u0022);\n }\n\n function renderSubFilter(subFilter, selected = null) {\n const organism = subFilter.organism;\n const importantList = [];\n for (const item of organism) {\n if (isImportant(item)) {\n item.is_important = true;\n importantList.unshift(item);\n const pos = organism.indexOf(item);\n organism.splice(pos, 1);\n }\n }\n for (const item of importantList) {\n organism.unshift(item);\n }\n const organismHtml = renderFilterUl(organism, \u0027species_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-organism-box\u0027).innerHTML = organismHtml;\n\n const tissueId = subFilter.tissue_id;\n const tissueHtml = renderFilterUl(tissueId, \u0027tissue_id\u0027);\n\n document.querySelector(\u0027#search-sub-filter-tissue-box\u0027).innerHTML = tissueHtml;\n\n const tissueSystem = subFilter.tissue_system;\n const tissueSystemHtml = renderFilterUl(tissueSystem, \u0027tissue_system\u0027);\n document.querySelector(\u0027#search-sub-filter-tissue_system-box\u0027).innerHTML = tissueSystemHtml;\n\n eventListener(\u0027.search-sub-filter-item\u0027, \u0027click\u0027, handleSubFilterClick);\n eventListener(\u0027.search-sub-filter-item-clear\u0027, \u0027click\u0027, handleClearSubFilter);\n }\n\n function isImportant(item) {\n const importantList = [\u0027Human\u0027, \u0027Mouse\u0027, \u0027Rat\u0027];\n for (const importantItem of importantList) {\n if (item.value.includes(importantItem)) {\n return true;\n }\n }\n return false;\n }\n\n function eventListener(selector, event, callback) {\n document.querySelectorAll(selector).forEach(el =\u003E {\n el.removeEventListener(event, callback);\n el.addEventListener(event, callback);\n });\n }\n\n function handleSubFilterClick() {\n if (!this.classList.contains(\u0027active\u0027)) {\n const key = this.dataset.key;\n const value = this.dataset.id;\n\n const state = history.state || {};\n state[key] = value;\n if (state.page) {\n delete state.page;\n }\n jump(state);\n }\n }\n\n function handleClearSubFilter(e) {\n e.stopPropagation();\n const parent = this.parentNode;\n const key = parent.dataset.key;\n const state = history.state;\n if (state \u0026\u0026 state.hasOwnProperty(key)) {\n delete state[key];\n }\n jump(state);\n }\n\n function renderFilterUl(items, key) {\n let html = \u0027\u003Cul\u003E\u0027;\n for (const item of items) {\n if (item.id \u0026\u0026 item.count \u003E 0) {\n html += `\n \u003Cli class=\u0022search-sub-filter-item ${item.selected}\u0022 data-key=\u0022${key}\u0022 data-id=\u0022${item.id}\u0022\u003E\n \u003Cspan\u003E${item.is_important ? (\u0027\u003Cstrong\u003E\u0027 + item.value + \u0027\u003C\/strong\u003E\u0027) : item.value}\u003C\/span\u003E\n \u003Cdiv class=\u0022search-sub-filter-item-clear\u0022\u003EX\u003C\/div\u003E\n \u003C\/li\u003E\n `;\n }\n }\n html += \u0027\u003C\/ul\u003E\u0027;\n\n return html;\n }\n\n function renderPaginate(currentPage, totalPage) {\n let html = \u0027\u0027;\n currentPage = Math.min(Math.max(1, currentPage), totalPage);\n\n if (totalPage !== 1) {\n html = \u0027\u003Cnav aria-label=\u0022Page navigation\u0022\u003E\u003Cul class=\u0022pager\u0022\u003E\u0027 +\n \u0027\u003Cli class=\u0022previous\u0027 + (currentPage === 1 ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage - 1) + \u0027\u0022\u003E\u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026larr;\u003C\/span\u003E Previous\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003Cli class=\u0022next\u0027 + (currentPage === totalPage ? \u0027 disabled\u0027 : \u0027\u0027) + \u0027\u0022\u003E\u003Ca href=\u0022javascript:void(0);\u0022 data-page=\u0022\u0027 + (currentPage + 1) + \u0027\u0022\u003ENext \u003Cspan aria-hidden=\u0022true\u0022\u003E\u0026rarr;\u003C\/span\u003E\u003C\/a\u003E\u003C\/li\u003E\u0027 +\n \u0027\u003C\/ul\u003E\u003C\/nav\u003E\u0027;\n }\n\n document.getElementById(\u0027paginate-container\u0027).innerHTML = html;\n\n eventListener(\u0027#paginate-container a\u0027, \u0027click\u0027, handlePaginateClick);\n }\n\n function handlePaginateClick() {\n if (this.parentNode.classList.contains(\u0027disabled\u0027)) {\n return;\n }\n const state = history.state || {};\n state.page = this.dataset.page;\n jump(state, \u0027result-box\u0027);\n }\n\n function disableFilterSection() {\n const el = document.querySelector(\u0027.search-box\u0027);\n el.style.position = \u0027relative\u0027;\n addShadow(el, \u0027shadow-search-box\u0027, true);\n\n const elResult = document.querySelector(\u0027.result-box\u0027);\n elResult.style.position = \u0027relative\u0027;\n addShadow(elResult, \u0027shadow-result-box\u0027);\n\n document.querySelector(\u0027.search-clear button\u0027).disabled = false;\n }\n\n function addShadow(elem, id, addLoading = false) {\n const el = document.getElementById(id);\n if (el) {\n el.style.display = \u0027block\u0027;\n el.style.opacity = 1;\n return;\n }\n\n elem.insertAdjacentHTML(\u0027beforeEnd\u0027, genearteShadow(id, addLoading));\n }\n\n function removeShadow(id) {\n const el = document.getElementById(id);\n if (el) {\n el.style.opacity = 0;\n setTimeout(function () {\n el.style.display = \u0027none\u0027;\n }, 500);\n }\n }\n\n function genearteShadow(id, addLoading = false) {\n let html = `\u003Cdiv id=\u0022${id}\u0022 style=\u0022position: absolute; left: 0; top: 0; right: 0; bottom: 0; background-color: rgba(255, 255, 255, .9); z-index: 100; transition: .5s;\u0022\u003E`;\n if (addLoading) {\n html += \u0027\u003Cdiv class=\u0022loading\u0022\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003Cdiv class=\u0022loading-item\u0022\u003E\u003C\/div\u003E\u003C\/div\u003E\u0027\n }\n html += `\u003C\/div\u003E`;\n\n return html;\n }\n\n function enableFilterSection() {\n removeShadow(\u0027shadow-search-box\u0027);\n removeShadow(\u0027shadow-result-box\u0027);\n document.querySelector(\u0027.search-clear button\u0027).disabled = !document.querySelector(\u0027.search-sub-filter-item.active\u0027);\n }\n\n search();\n\n document.querySelectorAll(\u0027input[name=\u0022search-filter_id\u0022]\u0027).forEach(el =\u003E {\n el.addEventListener(\u0027change\u0027, function () {\n if (el.classList.contains(\u0027prevent\u0027)) {\n return false;\n }\n\n document.querySelector(\u0027input[name=\u0022search-filter_id\u0022][value=\u0022\u0027 + DEFAULT_FILTER_ID + \u0027\u0022]\u0027).checked = true;\n\n switch (el.value + \u0027\u0027) {\n case \u002714\u0027:\n location.href = \u0027\/Immortalized-Cell-Lines.html\u0027;\n break;\n case \u002715\u0027:\n location.href = \u0027\/Tumor-Cell-Lines.html\u0027;\n break;\n case \u002716\u0027:\n location.href = \u0027\/Primary-Cells.html\u0027;\n break;\n default:\n jump({ filter_id: el.value });\n }\n });\n });\n\n document.querySelector(\u0027.search-clear button\u0027).addEventListener(\u0027click\u0027, () =\u003E {\n jump({\n filter_id: getDefaultFilterId()\n });\n });\n\n window.onpopstate = function () {\n \/\/ \u76f4\u63a5\u8fd4\u56de\u4e0a\u4e00\u7ea7\u9875\u9762\n history.back();\n return false;\n if (!history.state) {\n history.back();\n }\n search();\n }\n\u003C\/script\u003E\n\u003C\/p\u003E","meta_title":"Primary Cell Library","meta_keywords":"Primary Cells, human primary cells, animal primary cells, human cell lines, animal cell lines","meta_description":"abm\u2019s comprehensive cell collection provides primary and immortalized cell lines for your research needs. Continuously dividing immortalized cells arise from defined mutational events that allow the primary cells to evade normal cellular senescence. The resulting immortalized primary cells are highly useful for cell biology research as they are significantly easier to culture and maintain compared to their primary counterparts.","deleted_at":null,"enable":"Y","parent_list":"18","table_name":null,"image":null,"independentPage":0,"top_type":1,"sort_order":204,"in_footer":1,"fid":18,"created_at":null,"updated_at":"2025-02-24 00:17:52"},"info":{"id":363,"cat_no_base":"T5997","parent_id":34806,"image":"\/t\/5\/t5997-enhanced-primary-human-lsec-fig-3.jpg","price-original":null,"description":"\u003Cp\u003EEnhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells allowing for a reliable \u003Cem\u003Ein vitro\u003C\/em\u003E\u0026nbsp;research model. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for toxicity studies. The cells offer a unique ability to be kept in longer term culture when compared to standard primary liver sinusoidal endothelial cells which does not proliferate well,\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E.\u003Cbr \/\u003E\u003Cbr \/\u003EThe cells are ready-to-use after thawing. \u003Cstrong\u003Eabm \u003C\/strong\u003Erecommends passaging cells 1-2 times; beyond this limit cells may exhibit phenotypic changes and express senescence genes.\u003C\/p\u003E","shipment_type":null,"internal_leadtime":"1","lysate_type":null,"small_image":"\/t\/5\/t5997-enhanced-primary-human-lsec-fig-3.jpg","growth_factor_size":null,"gene_name_no_use":null,"mammalian_selection_marker":null,"mammalian_selection_text":null,"storage_condition":"Vapor phase of liquid nitrogen, or below -130\u00b0C.","str_profiles":null,"species_description":null,"cost":null,"note":"\u003Cp\u003EEnhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells which allows for a reliable \u003Cem\u003Ein vitro\u003C\/em\u003E system. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for toxicity studies. The cells offer a unique ability to be kept in longer term culture when compared to standard primary liver sinusoidal endothelial cells which does not proliferate well \u003Cem\u003Ein vitro\u003C\/em\u003E.\u003Cbr\/\u003E\u003Cbr\/\u003EThe cells are offered ready-to-use after thawing. Recommend to only passage the cells 1-2 more times, as more passages may see phenotypical changes and expression of senescence genes.\u003Cbr\/\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cbr\/\u003E\u003C\/p\u003E\u003Cp\u003ETo Thaw:\u003C\/p\u003E","family":null,"overexpressed_gene":null,"diameter":null,"appearance":null,"pluripotency":null,"recommended_medium_volume":null,"medium":"thawing media is upcyte (MHE001), growth media is upcyte (MLS003)\r\n*G422 coated*","alias":null,"tier_price":null,"operator_no_use":"angela_t","addtime":null,"cell_line":null,"price_type":null,"gallery":null,"cell_growth_area":null,"cell_morphology":"Endothelial-like","expression_system_type":null,"gene_symbol":null,"insert_size":null,"msrp":null,"msrp_display_actual_price_type":"0","cell_morphology_specification":null,"tax_class_id":2,"inventory_location":null,"vector":null,"internal_note":"\u003Cp\u003Eupcyte technologies (CLS002)\u003C\/p\u003E\n\u003Cp\u003EThawing media: MHE001\u003C\/p\u003E\n\u003Cp\u003EGrowth Media: MLS003 (min order quantity = 2x 500 ml - Purchase price: 1148.57 - need to get fresh quote per inquiry)\u003C\/p\u003E","component":null,"quantity_and_stock_status":null,"vector_url":null,"application":"\u003Cp\u003EResearch Use Only.\u003C\/p\u003E","minimal_price":null,"seeding_density":null,"seeding_density_cm":"10,000","seeding_density_ml":null,"source":null,"source_catno":"\u003Cp\u003ECLS002 ($2400 list price, 20% distributor discount to $1920)\u003C\/p\u003E","osmolarity":null,"weight":null,"royalty_rates":null,"population_doubling_time":null,"source_price":null,"weight_type":null,"ph":null,"licensor_commission_rate":null,"supplier":"Upcyte","donor_gender":null,"appearance_general":null,"sterility":null,"molecular_weight":null,"endotoxin_level":null,"donor_age":null,"news_from_date":null,"news_to_date":null,"expression_system_general":null,"donor_disease":null,"purity":null,"country_of_manufacture":null,"donor_ethnicity":null,"image_label":null,"biological_activity_text":null,"cart2quote_quotable":"2","biosafety":"II","product_quote":0,"small_image_label":null,"immortalization_method":null,"bioactivity_data":null,"formulation":null,"thumbnail_label":null,"mammalian_selection":null,"isolation_method":null,"function":null,"reconstitution":null,"growth_properties":"\u003Cp\u003EAdherent, endothelial-like\u003C\/p\u003E","expression_profile":null,"storage":null,"propagation_method":"\u003Cdiv style=\u0022color: red; font-weight: bold;\u0022\u003E\u003Cspan style=\u0022display: inline;\u0022\u003EUse of PriCoat\u003C\/span\u003E\u003Cstrong\u003E\u003Csup\u003E\u003Cspan style=\u0022font-size: 11pt; line-height: 115%; font-family: Calibri,sans-serif;\u0022\u003ETM\u003C\/span\u003E\u003C\/sup\u003E\u003C\/strong\u003E T25 Flasks (\u003Ca href=\u0022\/pricoat-coated-flask.html\u0022 target=\u0022_blank\u0022 rel=\u0022noopener noreferrer\u0022\u003EG299\u003C\/a\u003E) or Applied Cell Extracellular Matrix (\u003Ca href=\u0022\/applied-cell-extracellular-matrix-g422.html#g422\u0022 target=\u0022_blank\u0022 rel=\u0022noopener noreferrer\u0022\u003EG422\u003C\/a\u003E) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.\u003C\/div\u003E\n\u003Cp\u003EUse the ready-to-use Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111) and the Human Liver Sinusoidal Endothelial Cell Media Kit (TM112) which comes with the Human Liver Sinusoidal Endothelial Cell Basal Medium, and Human Liver Sinusoidal Endothelial Cell Supplement Mix available from\u0026nbsp;\u003Cstrong\u003Eabm\u003C\/strong\u003E.\u003Cbr \/\u003ECarbon dioxide (CO\u003Csub\u003E2\u003C\/sub\u003E): 5%, Temperature: 37.0\u0026deg;C.\u003C\/p\u003E\n\u003Cp\u003ETo make complete Human Liver Sinusoidal Endothelial Cell Growth Medium add the entire content of Human Liver Sinusoidal Endothelial Cell Supplement Mix into Human Liver Sinusoidal Endothelial Cell Basal Medium and mix properly in BioSafety Cabinet. Addition of the Human Liver Sinusoidal Endothelial Cell Supplement Mix may make medium appear more opaque.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003ETo Thaw:\u003C\/strong\u003E\u003Cbr \/\u003E1. Pre-warm Human Liver Sinusoidal Endothelial Cell Thawing Medium and fully supplemented Human Liver Sinusoidal Endothelial Cell Growth Medium to room temperature.\u003Cbr \/\u003E2. Carefully remove cryovial from storage tank.\u003Cbr \/\u003E3. Thaw cells in 37\u0026deg;C water bath until only a small chunk of ice is left. \u003Cem\u003EDo not shake the vial, or take it out of the water during thawing, as this will damage the cells.\u003C\/em\u003E\u003Cbr \/\u003E4. Spray the vial and the tube containing 50 ml of thawing medium with 70% ethanol and transfer to a Biosafety cabinet.\u003Cbr \/\u003E5. Transfer the now completely thawed cell suspension from the cryovial into 10 ml Human Liver Sinusoidal Endothelial Cell Thawing Medium in a new 50 ml conical tube by gently pipetting the cells into the medium using a 2 ml pipette.\u003Cbr \/\u003E6. Use a 1 ml pipette to transfer 1 ml of the thawing medium with the cells back to the cryovial and pipette the contents back into the 50 ml tube. Repeat this process twice to completely remove the cells from the cryovial.\u003Cbr \/\u003E7. Pellet the cells by centrifuging at 620\u0026times;g for 5 min at RT. \u003Cstrong\u003EImportant note:\u003C\/strong\u003E Higher g-forces will significantly reduce cell recovery.\u003Cbr \/\u003E8. Aspirate the supernatant without disturbing the pellet. Leave approximately 200 \u0026mu;l medium on top of the cells.\u003Cbr \/\u003E9. Add 800 \u0026mu;l of the fully supplemented Human Liver Sinusoidal Endothelial Cell Growth Medium and gently loosen and re-suspend the cells by pipetting them up and down 1-2 times.\u0026nbsp;\u003Cem\u003EDo not vortex or shake the cells as this will compromise cell survival.\u003C\/em\u003E\u003Cbr \/\u003E11. Determine viable cell number by cell count.\u003Cbr \/\u003E12. Dilute the Enhanced Primary Human Liver Sinusoidal Endothelial Cells in pre-warmed, fully supplemented Human Liver Sinusoidal Endothelial Cell Growth Medium and seed at ~10,000 cells\/cm\u003Csup\u003E2\u003C\/sup\u003E\u0026nbsp;in ECM-coated cell culture flasks (\u003Ca href=\u0022\/applied-cell-extracellular-matrix-g422.html#g422\u0022\u003EG422\u003C\/a\u003E) or appropriate cell culture dishes.\u003Cbr \/\u003E13. Incubate the cells at 95% humidity, 37\u0026deg;C and 5% CO\u003Csub\u003E2\u003C\/sub\u003E.\u003Cbr \/\u003E14. Next day, add fresh new Human Liver Sinusoidal Endothelial Cell Growth Medium. Conduct complete media change every other day with fresh Human Liver Sinusoidal Endothelial Cell Growth Medium for optimal growth.\u003C\/p\u003E","usage":null,"freeze_thaw_recovery":null,"str_profile":null,"str_markers":"","subculture_protocol":"\u003Cp\u003E\u003Cstrong style=\u0022text-wrap: wrap;\u0022\u003ECells are sensitive to trypsin; TrypLE Express is recommended for subculture procedures.\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\n\u003Cp\u003ECells can be passaged for \u003Cstrong\u003Eone\u003C\/strong\u003E subculture once they become 70-80% confluent. Do not expand cells furthers.\u0026nbsp;\u0026nbsp;\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EVolumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size.\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E1. Aspirate the culture media, wash the adherent monolayer with 1X PBS, then and add 2-3ml of pre-warmed TrypLE Express to the culture vessel.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37\u0026deg;C, for several minutes to facilitate detachment.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E3. Neutralize TrypLE Express by adding an equal volume of the complete growth media into the culture vessel.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 280xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Seed cells at 1\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E0,000 cells\/cm\u003C\/span\u003E\u003Csup style=\u0022text-wrap: wrap;\u0022\u003E2\u0026nbsp;\u003C\/sup\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003Ein the appropriate G422 coated culture vessel.\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E6. Incubate the cells at the recommended conditions.\u003C\/p\u003E\n\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E7. Allow the cells to expand over the next 2-3 days then perform experimental assays as desired.\u0026nbsp;\u003C\/p\u003E","preservation_protocol":"We do not recommend freezing down the Enhanced Primary Human Liver Sinusoidal Endothelial Cells.","passage_number":null,"qc":"\u003Cp\u003EEach lot has been tested for:\u003C\/p\u003E\n\u003Col class=\u0022 list-paddingleft-2\u0022\u003E\n\u003Cli\u003E\n\u003Cp\u003E\u0026gt;90% plateability\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003EPost-thaw viability\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003ECMV, HIV, HBV, HCV, and Mycoplasma absence\u003C\/p\u003E\n\u003C\/li\u003E\n\u003Cli\u003E\n\u003Cp\u003ELiver sinusoidal endothelial cell markers via immunofluorescence staining: MMR+ and CD31+\u003C\/p\u003E\n\u003C\/li\u003E\n\u003C\/ol\u003E","shipping_conditions":"\u003Cp\u003EShip with dry ice.\u003C\/p\u003E","disclaimer_general":"\u003Cp\u003E1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at \u003Ca href=\u0022mailto:licensing@abmgood.com\u0022\u003Elicensing@abmgood.com\u003C\/a\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. All test parameters provided in the CoA are conducted using \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s standardized culture system and procedures. The stated values may vary under the end-user\u0027s culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 \u0026mu;g, Cat.# C207, $450.00) or cell lysate (100 \u0026mu;g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.\u003C\/p\u003E\n\u003Cp\u003E3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination\u0027s temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).\u003C\/p\u003E\n\u003Cp\u003E4. All of \u003Cstrong\u003Eabm\u003C\/strong\u003E\u0027s cell biology products are for research use ONLY and NOT for therapeutic\/diagnostic applications. \u003Cstrong\u003Eabm\u003C\/strong\u003E is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic\/diagnostic application(s). Please contact a technical service representative for more information.\u003C\/p\u003E\n\u003Cp\u003E5. \u003Cstrong\u003Eabm\u003C\/strong\u003E makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. \u003Cstrong\u003Eabm\u003C\/strong\u003E does not warrant that such information has been shown to be accurate.\u003C\/p\u003E\n\u003Cp\u003E6. \u003Cstrong\u003Eabm\u003C\/strong\u003E warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable \u003Cstrong\u003Eabm\u003C\/strong\u003E Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \u0022Warranty Period.\u0022\u003C\/p\u003E","caution":null,"transfection_reagents_type":null,"licensed_by":null,"licensed_from":null,"licensing_institution":null,"guarantee":null,"depositor":null,"licensor_name":null,"licensor_contact_information":null,"contract_termination_date":null,"links_purchased_separately":null,"samples_title":null,"links_title":null,"links_exist":null,"internal_supplier":"Upcyte ($2400 list price, 20% distributor discount to $1920), special MTA required","internal_product_note":null,"donor_history":null,"split_ratio":null,"expression":"\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EMMR+, CD31+\u003C\/span\u003E\u003C\/p\u003E","growth_conditions":"\u003Cp style=\u0022text-wrap: wrap;\u0022\u003E\u003Cspan style=\u0022color: rgb(255, 0, 0);\u0022\u003E\u003Cstrong\u003EPriCoat\u2122 ECM T25 Flasks (\u003Ca href=\u0022https:\/\/www.abmgood.com\/pricoat-ECM-t25-flasks.html\u0022 target=\u0022_self\u0022\u003EG999\u003C\/a\u003E) or Applied Cell Extracellular Matrix (\u003Ca href=\u0022https:\/\/www.abmgood.com\/applied-cell-extracellular-matrix-g422.html\u0022 target=\u0022_self\u0022\u003EG422\u003C\/a\u003E) are\u0026nbsp;\u003C\/strong\u003E\u003C\/span\u003E\u003Cspan style=\u0022text-decoration-line: underline; color: rgb(255, 0, 0);\u0022\u003E\u003Cstrong\u003Erequired\u003C\/strong\u003E\u003C\/span\u003E\u003Cspan style=\u0022color: rgb(255, 0, 0);\u0022\u003E\u003Cstrong\u003E\u0026nbsp;for cell adhesion to the culture vessels.\u0026nbsp;\u003C\/strong\u003E\u003C\/span\u003EHuman Liver Sinusoidal Endothelial Cell Thawing Medium (\u003Ca href=\u0022https:\/\/www.abmgood.com\/human-liver-sinusoidal-endothelial-cell-thawing-medium-tm111.html\u0022 target=\u0022_self\u0022\u003ETM111\u003C\/a\u003E) + Human Liver Sinusoidal Endothelial Cell Media Kit (\u003Ca href=\u0022https:\/\/www.abmgood.com\/human-liver-sinusoidal-endothelial-cell-media-kit-tm112.html\u0022 target=\u0022_self\u0022\u003ETM112\u003C\/a\u003E), 37.0\u00b0C, 5% CO\u2082.\u003C\/p\u003E\u003Cp style=\u0022text-wrap: wrap;\u0022\u003ENote: TM111 is required for optimal recovery of cells post-thaw. Cells are grown using TM112.\u0026nbsp;\u003C\/p\u003E","recommend":"","references":null,"cryopreservation":"\u003Cp\u003ECryopreservation Medium (TM024), or complete growth media with 10% DMSO.\u003C\/p\u003E","unpacking_storage_instructions":"\u003Cp\u003E1. Visually examine the packaging containers for signs of leakage or breakage.\u003C\/p\u003E\n\u003Cp\u003E2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130\u0026deg;C, preferably in liquid nitrogen vapor phase storage, until ready for use.\u003C\/p\u003E\n\u003Cp\u003ETo ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130\u0026deg;C or in liquid nitrogen vapor phase. Do not store at -70\u0026deg;C, as it will result in loss of viability.\u003C\/p\u003E","promotions":null,"sync_images":"","thawing_protocol":"\u003Cp\u003EPre-warm Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111) and fully supplemented Human Liver Sinusoidal Endothelial Cell Growth Medium (TM112) to room temperature.\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E1.\u0026nbsp;\u003C\/span\u003EThaw cells quickly in a 37\u0026deg;C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. \u003Cem\u003EDo not shake vial\u003C\/em\u003E.\u003C\/p\u003E\n\u003Cp\u003E2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.\u003C\/p\u003E\n\u003Cp\u003E3. Transfer the cell suspension into a 50ml sterile conical tube containing 20ml of pre-warmed, \u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EHuman Liver Sinusoidal Endothelial Cell Thawing Medium (TM111)\u003C\/span\u003E. Centrifuge cells at \u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E280\u0026times;g for \u003C\/span\u003E5 minutes.\u0026nbsp;\u003C\/p\u003E\n\u003Cp\u003E4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 1 ml of pre-warmed, complete \u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EHuman Liver Sinusoidal Endothelial Cell Growth Medium (TM112)\u003C\/span\u003E and seed at\u0026nbsp;\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E10,000 cells\/cm\u003C\/span\u003E\u003Csup style=\u0022text-wrap: wrap;\u0022\u003E2\u0026nbsp;\u003C\/sup\u003Ein the appropriate G422 coated culture vessel.\u003C\/p\u003E\n\u003Cp\u003E5. Incubate the cells at the recommended conditions.\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E6. After 24 hours, add fresh Human Liver Sinusoidal Endothelial Cell Growth Medium (TM112).\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003EChange media\u003Cspan style=\u0022text-wrap: wrap;\u0022\u003E\u0026nbsp;\u003C\/span\u003Eevery other day with fresh Human Liver Sinusoidal Endothelial Cell Growth Medium (TM112) until cells become 70-80% confluent.\u0026nbsp;\u003C\/span\u003E\u003C\/p\u003E","type_online":null,"parental_cell_line":null,"mutation_and_validations":null,"knockout_method":null,"clone_no":null,"created_at":"2022-09-07 08:13:18","updated_at":"2025-03-03 21:07:26","concentration":null},"seo":{"id":67516,"catalog_id":34806,"catalog_type":"App\\Models\\CatalogBaseCells","url_key":"enhanced-primary-human-liver-sinusoidal-endothelial-cells.html","meta_title":null,"meta_keywords":"Enhanced Primary Human Liver Sinusoidal Endothelial Cells","meta_description":"Enhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells which allows for a reliable \u003Ci\u003Ein vitro\u003C\/i\u003E system. These cells are ready-to-use for applications","created_at":"2022-09-23 06:53:13","updated_at":"2024-05-13 19:44:57"},"media":[{"id":370586,"parent_id":34806,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/t\/5\/t5997-enhanced-primary-human-lsec-fig-1.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":1,"status":0,"entity_id_m2":18048428,"sku_in_m2":"T5997","value_id_m2":49500472,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":370587,"parent_id":34806,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/t\/5\/t5997-enhanced-primary-human-lsec-fig-2.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":2,"status":0,"entity_id_m2":18048428,"sku_in_m2":"T5997","value_id_m2":49500473,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":370588,"parent_id":34806,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/t\/5\/t5997-enhanced-primary-human-lsec-fig-3.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":3,"status":0,"entity_id_m2":18048428,"sku_in_m2":"T5997","value_id_m2":49500474,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"},{"id":370589,"parent_id":34806,"parent_type":"App\\Models\\CatalogBaseCells","file_path":"\/c\/e\/cell-culture-overview.jpg","title":null,"text":null,"file_type":"image","alt":null,"url":null,"position":4,"status":0,"entity_id_m2":18048428,"sku_in_m2":"T5997","value_id_m2":49500475,"attribute_id":90,"created_at":"2022-07-19 04:36:22","updated_at":"2022-07-19 05:37:56"}],"gene":null}