GFP Adenovirus

  • Product Name

    GFP Adenovirus
  • Unit

    1.0 ml
  • Description

    The 000541A GFP adenovirus is part of abm’s Adenoviral Expression System and can be used directly to transiently over-express eGFP in a wide range of host cells. The enhanced GFP displays strong fluorescence under the control of the CMV promoter, and can be used as a expression control for other recombinant adenoviruses. 

  • Vector Map

    pAdeno (click blue link to view)
  • Titer

    106 pfu/mL
  • Storage Condition

    DMEM with 10% glycerol

  • Disclaimer

    DeBosch, BJ et al. "Glucose transporter 8 (GLUT8) mediates fructose-induced de novo lipogenesis and macrosteatosis." J Biol Chem 289(16):10989-10998 (2014). DOI: 10.1074/jbc.M113.527002. PubMed: 24519932. Application: Transduction.
    Soliman, H et al. "Diabetes-induced increased oxidative stress in cardiomyocytes is sustained by a positive feedback loop involving Rho kinase and PKCβ2" Am. J. Physiol. Heart Circ. Physiol. 303:H989 - H1000 (2012). DOI: 10.1152/ajpheart.00416.2012. PubMed: 22865386. Application: Tranduction.
    Gyuyoup, K et al. "Transient Intermittent Hypoxia Exposure Disrupts Neonatal Bone Strength" Front Pediatr. 4.15:N/A (2016). DOI: 10.3389/fped.2016.00015. Application: Expression and WB.
    Laconetti, C et al. "Down-regulation of miR-23b induces phenotypic switching of vascular smooth muscle cells in vitro and in vivo" Cardiovascular Research :522-533 (2015). DOI: 10.1093/cvr/cvv141. Application: Transduction.
    Zhu, Q et al. "USP7 deubiquitinase promotes ubiquitin-dependent DNA damage signaling by stabilizing RNF168" Cell Cycle 14.9:1413-1425 (2016). DOI: 10.1080/15384101.2015.1007785. Application: Expression and WB.
    Hai, B et al. "Rescue effects and underlying mechanisms of intragland Shh gene delivery on irradiation-induced hyposalivation" Hum. Gene Ther. 27.5:390-399 (2016). DOI: 10.1089/hum.2016.005. Application: Infection.
    Vernia, S et al. "An alternative splicing program promotes adipose tissue thermogenesis" Elife 5:N/A (2016). DOI: 10.7554/eLife.17672. Application: Infection.
    Hwang, S., He, Y., Xiang, X., Seo, W., Kim, S., Ma, J., … Gao, B. "Interleukin‐22 ameliorates neutrophil‐driven nonalcoholic steatohepatitis through multiple targets" Hepatology. : (2019). DOI: 10.1002/hep.31031 .
    Li, E., Wang, L., Wang, D., Chi, J., Lin, Z., Smith, G. I., Klein, S., Cohen, P., & Rosen, E. D. (2023). Control of lipolysis by a population of oxytocinergic sympathetic neurons. Nature, 625(7993), 175–180. https://doi.org/10.1038/s41586-023-06830-x
    Nguyen, H. P., Villivalam, S. D., Jung, B. C., You, D., Lin, F., Yi, D., Pi, A., Ma, K., Jung, S., Park, S.-H., Jang, C., Sul, H. S., & Kang, S. (2022). AIFM2 is required for high-intensity aerobic exercise by promoting glucose utilization. https://doi.org/10.2337/figshare.20173055.v1
    Fang, X.-Q., Kim, Y.-S., Lee, Y.-M., Lee, M., Lim, W.-J., Yim, W.-J., Han, M.-W., & Lim, J.-H. (2022). Polygonum cuspidatum Extract (Pc-Ex) Containing Emodin Suppresses Lung Cancer-Induced Cachexia by Suppressing TCF4/TWIST1 Complex-Induced PTHrP Expression. Nutrients, 14(7), 1508. https://doi.org/10.3390/nu14071508
    Naren Qimuge, He, Z., Qin, J., Sun, Y., Wang, X., Yu, T., Dong, W., Yang, G., & Pang, W. (2019). Overexpression of DNMT3A promotes proliferation and inhibits differentiation of porcine intramuscular preadipocytes by methylating p21 and PPARg promoters. 696, 54–62. https://doi.org/10.1016/j.gene.2019.02.029
    Ishikawa, T., Takemoto, M., Akimoto, Y., Takada-Watanabe, A., Yan, K., Sakamoto, K., Maezawa, Y., Suguro, M., He, L., Tryggvason, K., Betsholtz, C., & Yokote, K. (2021). A novel podocyte protein, R3h domain containing-like, inhibits TGF-β-induced p38 MAPK and regulates the structure of podocytes and glomerular basement membrane. Journal of Molecular Medicine, 99(6), 859–876. https://doi.org/10.1007/s00109-021-02050-w
    ABM community
    Verified customer
    Asked on Feb 28 2025
    Answer
    abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
    ABM Scientific Support
    Answered on Feb 28 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.

    MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The concentration must be optimized for each cell type.  Typical selection amounts are around 0.1 - 0.5µg per ml.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
    ABM Scientific Support
    Answered on Mar 24 2025
    ABM community
    Verified customer
    Asked on Mar 24 2025
    Answer
    MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
    ABM Scientific Support
    Answered on Mar 24 2025
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Current vector selected:
GFP Adenovirus
Cat. No.
000541A
Controls and Related Products
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3rd Generation Packaging System Mix
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AAViralEntry™ Transduction Enhancer (100X)
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ViralEntry™ Transduction Enhancer (100X)
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Empty
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Current vector selected:
Cat. No.
Controls and Related Products
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
2nd Generation Packaging System Mix
LV003
200µl
$293.00
3rd Generation Packaging System Mix
LV053
200µl
$355.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
ViralEntry™ Transduction Enhancer (100X)
G515
1.0 ml
$190.00
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