Human Cardiomyocytes
| Cat. No. | T5004 |
| Name | Human Cardiomyocytes |
| Description |
Human Cardiomyocytes are derived from human embryonic stem cells (hESCs) through a robust and reproducible differentiation process. These highly functional cells exhibit characteristic cardiomyocyte morphology, spontaneous contractile activity, and expression of key cardiac markers, including cTnI and α-actinin. Designed for in vitro cardiac research, they are ideal for drug screening, cardiotoxicity testing, disease modeling, cardiac physiology studies, and tissue engineering. Following thaw, cells attach and recover within 24–72 hours and typically begin beating spontaneously within 3–5 days, maintaining stable, synchronized contractions thereafter. With excellent scalability, consistency, and a human-relevant phenotype, these hESC-derived cardiomyocytes provide a reliable alternative to primary cardiomyocytes and animal models. Critical: Cardiomyocyte contractility is highly dependent on cell density. A seeding density of 2.1 × 10⁵ cells/cm² is recommended to promote sufficient cell-cell coupling and the development of a synchronized, spontaneously beating monolayer. Lower seeding densities may not support detectable beating. Explore the performance data and validation images here. |
| Organism | Human (H. sapiens) |
| Tissue | Heart |
| Storage Condition | Vapor phase of liquid nitrogen, or below -130°C. |
| Shipping Conditions | Ship with dry ice. |
| Product Format | Frozen |
| Intended Use | This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. |
| BioSafety | II |
| Certificate of Analysis | For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com. |
| Growth Conditions |
For optimal post-thaw recovery and culture, coat culture vessels with 1% 3DCelMatrix™ (TM076) using cold DMEM/F-12 Medium (TM510). Incubate coated vessels at 37°C for 2 hours prior to use. Immediately before plating, aspirate the coating solution and add pre-warmed Human Cardiomyocyte Maintenance Medium Kit (TM212) supplemented 10 µM ROCK Inhibitor Y-27632 (TM131) with 1% Penicillin/Streptomycin Solution (G255). Culture cells at 37°C, 5% CO₂. Perform a gentle medium change (without ROCK Inhibitor) 24 hours post-thaw to remove debris and dead cells, taking care not to disturb weakly attached cells. Replace medium every other day with fresh Human Cardiomyocyte Maintenance Medium Kit. Cells attach and recover within 24–72 hours post-thaw and begin spontaneous beating within 3–5 days, with stable, synchronous contractions maintained thereafter.
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| Unpacking and Storage Instructions |
1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use. To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability. |
| Thawing Protocol |
1. Pre-warm Human Cardiomyocyte Maintenance Medium Kit (TM212) supplemented with 1% Penicillin/Streptomycin Solution (G255) to 37°C. 2. Prepare 3DCelMatrix™-coated culture vessels as described under Growth Conditions. Immediately before thawing cells, aspirate the coating solution and add pre-warmed maintenance medium to each well. For a 6-well plate, add approximately 2-3 ml medium per well. Keep plates at 37°C until plating. 3. Quickly transfer the frozen vial to a 37°C water bath. Gently swirl until only a small ice crystal remains, approximately 1-2 minutes. Keep the vial cap above the water level to minimize contamination risk. 4. Spray and wipe the vial exterior with 70% ethanol, then transfer the vial into a biological safety cabinet. From this point onward, perform all steps using aseptic technique. 5. Transfer the cell suspension dropwise into a sterile 15 ml conical tube containing 9-10 ml of pre-warmed maintenance medium while gently swirling the tube. 6. Centrifuge at 200 x g for 5 minutes at room temperature. 7. Carefully aspirate the supernatant without disturbing the cell pellet. 8. Resuspend the cell pellet in 1 ml of pre-warmed maintenance medium. Gently pipette 2-3 times to obtain small, uniform clusters. Avoid large aggregates, and do not fully dissociate the cells into single cells. 9. Plate cells at 2×10⁶ viable cells per well in a standard 6-well plate. Gently distribute cells by swirling the plate in a cross pattern. Do not swirl circularly. 10. Incubate at 37°C, 5% CO₂. At 24 hours post-plating, perform a gentle medium change with fresh cardiomyocyte maintenance medium. Replace medium every other day thereafter. |
| Subculture Protocol |
Cells are terminally differentiated and are not intended for routine passaging. For optimal performance, use cells for experimental applications as soon as possible after initiating culture. During post-thaw recovery, avoid complete dissociation into single cells. |
| Seeding Density (cells/cm2) | 2.1 × 10⁵ |
| Expression |
cTnI and α-actinin |
| Warranty | abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”. |
| Disclaimer |
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 3. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 4. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period." |
| Application | Research Use Only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T5004 |